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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">agronauka</journal-id><journal-title-group><journal-title xml:lang="ru">Аграрная наука Евро-Северо-Востока</journal-title><trans-title-group xml:lang="en"><trans-title>Agricultural Science Euro-North-East</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2072-9081</issn><issn pub-type="epub">2500-1396</issn><publisher><publisher-name>FARC North-East</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.30766/2072-9081.2022.23.4.538-547</article-id><article-id custom-type="elpub" pub-id-type="custom">agronauka-1103</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ОРИГИНАЛЬНЫЕ СТАТЬИ: ЗЕМЛЕДЕЛИЕ, АГРОХИМИЯ, МЕЛИОРАЦИЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>ОRIGINAL SCIENTIFIC ARTICLES: AGRICULTURE, AGROCHEMISTRY, LAND IMPROVEMENT</subject></subj-group></article-categories><title-group><article-title>Ферментативная активность техногенных поверхностных образований Кузбасса</article-title><trans-title-group xml:lang="en"><trans-title>Enzymative activity of technogenic surface formations of Kuzbass</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-3988-8521</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Фролова</surname><given-names>А. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Frolova</surname><given-names>A. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>младший научный сотрудник лаборатории фиторемедиации техногенно нарушенных экосистем, </p><p>ул. Красная, д. 6, г. Кемерово, 650000</p><p>flyflyflyflyfly.af@mail.ru</p></bio><bio xml:lang="en"><p>junior researcher, the Laboratory of Phytoremediation of Technogenically Disturbed Ecosystems,</p><p>Krasnaya str., 6, Kemerovo, 650000</p></bio><email xlink:type="simple">rector@kemsu.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-5846-6557</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Переверзева</surname><given-names>М. К.</given-names></name><name name-style="western" xml:lang="en"><surname>Pereverzeva</surname><given-names>M. K.</given-names></name></name-alternatives><bio xml:lang="ru"><p>младший научный сотрудник лаборатории фиторемедиации техногенно нарушенных экосистем, </p><p>ул. Красная, д. 6, г. Кемерово, 650000</p></bio><bio xml:lang="en"><p>junior researcher, the Laboratory of Phytoremediation of Technogenically Disturbed Ecosystems, </p><p>Krasnaya str., 6, Kemerovo, 650000</p></bio><email xlink:type="simple">rector@kemsu.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-4988-8197</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Асякина</surname><given-names>Л. К.</given-names></name><name name-style="western" xml:lang="en"><surname>Asyakina</surname><given-names>L. K.</given-names></name></name-alternatives><bio xml:lang="ru"><p>кандидат технических наук, заведующий лаборатории фиторемедиации техногенно нарушенных экосистем, </p><p>ул. Красная, д. 6, г. Кемерово, 650000</p></bio><bio xml:lang="en"><p>PhD in Engineering, Head of the Laboratory of Phytoremediation of Technogenically Disturbed Ecosystems, </p><p>Krasnaya str., 6, Kemerovo, 650000</p></bio><email xlink:type="simple">rector@kemsu.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-2958-4172</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Голубцова</surname><given-names>Ю. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Golubtsova</surname><given-names>Yu. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>доктор технических наук, проректор по развитию имущественного комплекса,</p><p>ул. Красная, д. 6, г. Кемерово, 650000</p></bio><bio xml:lang="en"><p>DSc in Engineering, vice-rector for the development of the property complex,</p><p>Krasnaya str., 6, Kemerovo, 650000</p></bio><email xlink:type="simple">rector@kemsu.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-4045-8054</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Осинцева</surname><given-names>М. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Osintseva</surname><given-names>M. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>кандидат технических наук, начальник управления проектной деятельностью,</p><p>ул. Красная, д. 6, г. Кемерово, 650000</p></bio><bio xml:lang="en"><p>PhD in Engineering, Head of the Department of Project Activity,</p><p>Krasnaya str., 6, Kemerovo, 650000</p></bio><email xlink:type="simple">rector@kemsu.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>ФГБОУ ВО «Кемеровский государственный университет»</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Kemerovo State University</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2022</year></pub-date><pub-date pub-type="epub"><day>25</day><month>08</month><year>2022</year></pub-date><volume>23</volume><issue>4</issue><fpage>538</fpage><lpage>547</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Фролова А.С., Переверзева М.К., Асякина Л.К., Голубцова Ю.В., Осинцева М.А., 2022</copyright-statement><copyright-year>2022</copyright-year><copyright-holder xml:lang="ru">Фролова А.С., Переверзева М.К., Асякина Л.К., Голубцова Ю.В., Осинцева М.А.</copyright-holder><copyright-holder xml:lang="en">Frolova A.S., Pereverzeva M.K., Asyakina L.K., Golubtsova Y.V., Osintseva M.A.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.agronauka-sv.ru/jour/article/view/1103">https://www.agronauka-sv.ru/jour/article/view/1103</self-uri><abstract><p>Угольная промышленность является одной из ведущих отраслей в мировом энергобалансе. Главным регионом России, специализирующимся на угледобыче, является Кузбасс, где добывают около 60 % всего угля страны. Добыча на территории Кемеровской области осуществляется в основном открытым способом, в результате чего образуются нарушенные земли, площадь которых составляет порядка 178 тыс. га. Индикатором самовосстановления почв является ферментативная активность. Цель работы ‒ изучение ферментативной активности техногенных поверхностных образований Кузбасса для оценки их токсичности и дальнейшего подбора микроорганизмовдеструкторов, ризобактерий и растений-гипераккумуляторов, которые будут применяться на биологическом этапе рекультивации. Материалом для исследования послужили образцы техногенных поверхностных образований, отобранные на территории Барзасского и Моховского угольных отвалов. Ферментативная активность техногенно нарушенных образований отвалов составила: инвертаза – 2,24 и 2,12 мг сахарозы, расщепленной 1 г почвы за 1 час; нитритредуктаза – 0,57 и 0,07 мг восстановленного NO2- на 1 г почвы за 24 часа; аспарагиназа –71,22 и 60,63 мг NH3 на 1 г почвы за 24 часа соответственно. При изучении ферментативной активности сделали предположение – аборигенная микрофлора использует в качестве источников углерода низко- и высокомолекулярные углеводороды (алканы, полициклические ароматические углеводороды (ПАУ) и др.). Содержание подвижных форм тяжелых металлов (ТМ) в изучаемых образцах превышает предельно допустимые концентрации в 1,2-2,6 раза. В ходе статистического анализа выявлено, что валовые и подвижные формы цинка и меди являются инигибиторами нитритредуктазы, валовая и подвижная формы никеля – активаторами аспарагиназы в техногенных нарушенных образованиях исследуемых угольных отвалов, а также ингибиторами инвертазы в Моховском угольном отвале. </p></abstract><trans-abstract xml:lang="en"><p>The coal mining is one of the leading industries in the global energy balance. Kuzbass is the main region of Russia that specializes in coal mining. About 60 % of the country's coal is mined there. Coal mining is carried out mainly by the open-pit method. As a result, some 178 thousand hectares ha of disturbed land are formed. Enzymatic activity is an indicator of soil self-repair. The aim of the work was to study the enzymatic activity of technogenic surface formations of the Kuzbass to assess their toxicity and further selection of destructor microorganisms, rhizobacteria and hyperaccumulator plants, which will be further used at the biological stage of recultivation. As objects of research, the samples of technogenic surface formations taken on the territory of the Barzassky and Mokhovsky coal dumps. Enzymatic activity of technogenic surface formations of dumps was: invertase – 2,24 and 2,12 mg of sucrose split 1 g soil in 1 h; nitrite reductase – 0,57 and 0,07 mg reduced NO2- per 1 g soil in 24 h; asparaginase – 71,22 and 60,63 mg NH3 per 1 g soil in 24 h, respectively. When studying the enzymatic activity, it was assumed that the native microflora uses low- and high-molecular hydrocarbons (alkanes, polycyclic aromatic hydrocarbons (PAH), etc.) as carbon sources. The content of mobile forms of heavy metals (HM) in the studied samples exceeds the maximum allowable concentration by 1,2-2,6 times. In the course of statistical analysis, it was revealed that the gross and mobile forms of zinc and copper are nitrite reductase inhibitors, the gross and mobile form of nickel is an asparaginase activator, in technogenic disturbed formations of the studied coal dumps. Nickel is also an invertase inhibitor in the Mokhovsky coal dump. </p></trans-abstract><kwd-group xml:lang="ru"><kwd>угольные отвалы</kwd><kwd>загрязненная почва</kwd><kwd>тяжелые металлы</kwd><kwd>инвертаза</kwd><kwd>нитритредуктаза</kwd><kwd>аспарагиназа</kwd></kwd-group><kwd-group xml:lang="en"><kwd>coal dumps</kwd><kwd>polluted soil</kwd><kwd>heavy metals</kwd><kwd>invertase</kwd><kwd>nitrite reductase</kwd><kwd>asparaginase</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Работа выполнена при поддержке Минобрнауки РФ (тема FZSR-202100).</funding-statement><funding-statement xml:lang="en">The work was carried out with the support of the Ministry of Education and Science of the Russian Federation (theme FZSR-2021-00).</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Фасхутдинова Е. Р., Осинцева М. А., Неверова О. А. 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