agronaukaАграрная наука Евро-Северо-ВостокаAgricultural Science Euro-North-East2072-90812500-1396FARC North-East10.30766/2072-9081.2023.24.5.849-857agronauka-1454Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИ: ЗООТЕХНИЯОRIGINAL SCIENTIFIC ARTICLES: ZOOTECHNYЛокусы панели генотипирования секвенированием по технологии AgriSeq в породе манычский мериносLoci of the genotyping panel by sequencing using AgriSeq technology in the Manych Merino breedhttps://orcid.org/0000-0003-4536-1814КриворучкоА. Ю.KrivoruchkoA. Yu.

Криворучко Александр Юрьевич, доктор биол. наук, главный научный сотрудник; научный сотрудник

 д. 49, ул. Никонова, г. Михайловск, Ставропольский край, 356241;

д. 2, корп. 23, просп. Кулакова, г. Ставрополь, 355029

Alexander Yu. Krivoruchko, DSc in Biology, chief researcher

Centre, 49, Nikonova Street, Mikhailovsk, Stavropol Territory, 356241;

 2, bldg. 23, ave. Kulakova, Stavropol, 355029

 

rcvm@yandex.ruhttps://orcid.org/0000-0002-2190-301XЛиховидА. А.LikhovidA. A.

Лиховид Андрей Александрович, доктор географ. наук,  профессор

д. 2, корп. 23, просп. Кулакова, г. Ставрополь, 355029

Andrey A. Likhovid, DSc in Geographic, professor

 2, bldg, 23, ave, Kulakova, Stavropol, 355029

info@ncfu.ruhttps://orcid.org/0000-0003-3003-4175КаниболоцкаяА. А.KanibolotskayaA. A.

Каниболоцкая Анастасия Александровна, кандидат биол. наук, старший научный сотрудник

д. 49, ул. Никонова, г. Михайловск, Ставропольский край, 356241

Anastasia A. Kanibolotskaya, PhD in Biology, senior researcher

49, Nikonova Street, Mikhailovsk, Stavropol Territory, 356241

info@fnac.centerhttps://orcid.org/0000-0002-5306-795XСаприкинаТ. Ю.SaprikinaT. Yu.

Саприкина Татьяна Юрьевна, аспирант, младший научный сотрудник

49, ул. Никонова, г. Михайловск, Ставропольский край, 356241

 

Tatyana Yu. Saprikina, postgraduate student, junior researcher

49, Nikonova Street, Mikhailovsk, Stavropol Territory, 356241

info@fnac.centerhttps://orcid.org/0000-0003-4093-5807КухарукМ. Ю.KuharukM. Yu.

Кухарук Максим Юрьевич, кандидат биол. наук, и.о. заведующего кафедрой

д. 2, корп. 23, просп. Кулакова, г. Ставрополь, 355029

Maxim Yu. Kuharuk, PhD in Biology, acting head of the department

2, bldg. 23, ave. Kulakova, Stavropol, 355029

info@ncfu.ruhttps://orcid.org/0000-0003-2730-2482ЯцыкО. А.YatsykO. A.

Яцык Олеся Андреевна, кандидат биол. наук, старший научный сотрудник

д. 49, ул. Никонова, г. Михайловск, Ставропольский край, 356241

Olesya A. Yatsyk, PhD in Biology, senior researcher

49, Nikonova Street, Mikhailovsk, Stavropol Territory, 356241

info@fnac.centerФГБНУ «Северо-Кавказский федеральный научный аграрный центр»; ФГАОУ ВО «Северо-Кавказский федеральный университет»РоссияNorth Caucasus Federal Agrarian Research Centre; North Caucasus Federal UniversityRussian FederationФГАОУ ВО «Северо-Кавказский федеральный университет»РоссияNorth Caucasus Federal UniversityRussian FederationФГБНУ «Северо-Кавказский федеральный научный аграрный центр»РоссияNorth Caucasus Federal Agrarian Research CentreRussian Federation202331102023245849857Copyright © Криворучко А.Ю., Лиховид А.А., Каниболоцкая А.А., Саприкина Т.Ю., Кухарук М.Ю., Яцык О.А., 20232023Криворучко А.Ю., Лиховид А.А., Каниболоцкая А.А., Саприкина Т.Ю., Кухарук М.Ю., Яцык О.А.Krivoruchko A.Y., Likhovid A.A., Kanibolotskaya A.A., Saprikina T.Y., Kuharuk M.Y., Yatsyk O.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.agronauka-sv.ru/jour/article/view/1454

С использованием генотипирования баранов породы манычский меринос на базе Illumina BeadChip Ovine 600K были обнаружены локусы, пригодные для генотипирования секвенированием животных этой породы. Выявлены однонуклеотидные полиморфизмы (SNP) с высокой частотой встречаемости в диапазоне 0,2850-0,3149 гомозигот как диких, так и мутантных вариантов. Гетерозиготные варианты этих замен встречались с частотой 0,379±0,012. Количество соответствующих выбранным критериям полиморфизмов составило 521. Анализ расположения обнаруженных SNP в геноме овец показал их наличие по всей длине генотипируемой области ДНК. Наибольшее количество полиморфизмов находилось на 1, 2, 3, 17 и Х хромосомах. Меньше всего полиморфизмов выявлено на 18, 21, 24 и 25 хромосомах. Полученный набор замен позволит эффективно решать задачи подтверждения достоверности происхождения овец породы манычский меринос, точно идентифицировать животных в процессе селекционной работы, проводить учет инбридинга в популяции. Предложенный нами набор SNP рекомендуется как для использования в генотипировании секвенированием нового поколения, так и для кастомизации SNP-биочипов.

Using the genotyping of sheep of the Manych Merino breed on the basis of Illumina BeadChip Ovine 600K, loci suitable for genotyping by sequencing animals of this breed were found. Single nucleotide polymorphisms with a high frequency of occurrence in the range of 0.2850-0.3149 homozygotes of both wild and mutant variants were identified. Heterozygous variants of these substitutions occurred with a frequency of 0.379±0.012. The number of polymorphisms corresponding to the selected criteria was 521. Analysis of the location of the detected SNPs in the sheep genome showed their presence along the entire length of the genotyped DNA region. The largest number of polymorphisms were found on chromosomes 1, 2, 3, 17 and X. The least polymorphisms were detected on chromosomes 18, 21, 24 and 25. The resulting set of substitutions will effectively solve the problems of confirming the authenticity of the origin of sheep of the Manych Merino breed, accurately identify animals in the process of breeding work, and account for inbreeding in the population. The proposed set of SNPs is recommended both for use in genotyping by sequencing of a new generation, and for customization of SNP biochips.

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The authors declare that there are no conflicts of interest present.