agronaukaАграрная наука Евро-Северо-ВостокаAgricultural Science Euro-North-East2072-90812500-1396FARC North-East10.30766/2072-9081.2023.24.6.989-998agronauka-1484Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИ: СЕЛЬСКОХОЗЯЙСТВЕННАЯ МИКРОБИОЛОГИЯ И МИКОЛОГИЯОRIGINAL SCIENTIFIC ARTICLES: AGRICULTURAL MICROBIOLOGY AND MYCOLOGYБактериальное сообщество сельскохозяйственных почв, используемых для возделывания картофеля в Свердловской областиBacterial community of agricultural soils used for potato cultivation in Sverdlovsk regionhttps://orcid.org/0009-0000-5818-3813ШанинаЕ. П.ShaninaE. P.

Шанина Елена Петровна, доктор с.-х. наук, главный научный сотрудник

д. 21, ул. Главная, г. Екатеринбург, 620061

Elena P. Shanina, DSc in Agricultural Science, chief researcher

21, Glavnaya St., Yekaterinburg, 620061

uralniishoz@list.ruhttps://orcid.org/0000-0003-2616-2166ЛиходеевскийГ. А.LihodeevskiyG. A.

Лиходеевский Георгий Александрович, младший научный сотрудник

д. 21, ул. Главная, г. Екатеринбург, 620061

Georgiy A. Lihodeevskiy, junior researcher

21, Glavnaya St., Yekaterinburg, 620061

georglihodey@gmail.comФГБНУ «Уральский федеральный аграрный научно-исследовательский центр Уральского отделения Российской академии наук»РоссияUral Federal Agrarian Scientific Research Centre, Ural Branch of the Russian Academy of SciencesRussian Federation202316122023246989998Copyright © Шанина Е.П., Лиходеевский Г.А., 20232023Шанина Е.П., Лиходеевский Г.А.Shanina E.P., Lihodeevskiy G.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.agronauka-sv.ru/jour/article/view/1484

На урожайность картофеля и других сельскохозяйственных культур оказывает влияние множество факторов, один из главнейших – комплексное состояние почвы. В исследованиях почв чаще делают акцент на определение ее физико-химических свойств, но редко учитывают бактериальное сообщество и его разнообразие. В работе проведена оценка бактериальной микробиоты почв, на которых возделывается картофель. С помощью метабаркодирования и полнофрагментного секвенирования участка 16S рРНК, методом нанопорового секвенирования в 2022 г. был проведён первичный скрининг бактериального сообщества полей в трёх административных районах Свердловской области: город Екатеринбург, Белоярский и Сысертский районы. В результате до уровня вида была определена 2371 операционная таксономическая единица (ОТЕ). Более половины относительного бактериального обилия занимал филум Proteobacteria. Более трети всего бактериального сообщества приходилось на три порядка: Burkholderiales, Hyphomicrobiales и Acidobacteriales. Наиболее распространены в обрабатываемых сельскохозяйственных почвах Свердловской области рода бактерий: Bradyrhizobium, Massilia, Gaiella, Sphingomonas, Lysobacter и Gemmatimonas. Полученные результаты анализа альфа- и бета-разнообразия позволили заключить, что, несмотря на статистически значимую разницу в численности обнаруженных ОТЕ между некоторыми полями, различие в их разнообразии по объектам исследования в административных районах Свердловской области отсутствует.

The yield of potatoes and other crops is influenced by many factors, one of the most important is the complex condition of the soil. Soil research more often focuses on the determination of its physical and chemical properties, but rarely takes into account the bacterial community and its diversity. In this work, the bacterial microbiota of soils cultivated with potato was evaluated. Using metabarcoding and full-fragment sequencing of the 16S rRNA site, by nanopore sequencing, primary screening of the bacterial community of fields in three administrative districts of the Sverdlovsk region: the city of Yekaterinburg, Beloyarsky and Sysertsky districts was carried out in 2022. As a result, 2371 operational taxonomic units (OTUs) were identified to the species level. More than half of the relative bacterial abundance is occupied by the phylum Proteobacteria. Three orders represent more than one-third of the total bacterial community: Burkholderiales, Hyphomicrobiales, and Acidobacteriales. The most common bacterial genera in cultivated agricultural soils of the Sverdlovsk region are Bradyrhizobium, Massilia, Gaiella, Sphingomonas, Lysobacter and Gemmatimonas. The obtained results of alpha- and beta-diversity analysis allow us to conclude that, despite the statistically significant difference in the number of detected OTUs between some fields, there is no difference in their diversity by study objects in the administrative districts of the Sverdlovsk region.

Solanum tuberosum L.микробиотапочвабиоразнообразиесеквенированиеSolanum tuberosum L.microbiotasoilbiodiversitysequencingРабота выполнена при поддержке Минобрнауки России в рамках выполнения подпрограммы КПНИ «Селекция и семеноводство картофеля». Авторы благодарят рецензентов за их вклад в экспертную оценку данной работы.The work was carried out with the support of the Ministry of Science and Higher Education of the Russian Federation as part of the implementation of the CSRP subprogram "Breeding and seed production of potatoes". The authors thank the reviewers for their contribution to the expert evaluation of this work.ReferencesYoung I. M., Crawford J. W. Interactions and self-organization in the soil-microbe complex. Science. 2004;304(5677):1634-1637. DOI: https://www.science.org/doi/10.1126/science.1097394Young I. M., Crawford J. W. Interactions and self-organization in the soil-microbe complex. Science. 2004;304(5677):1634-1637. 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The authors declare that there are no conflicts of interest present.