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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">agronauka</journal-id><journal-title-group><journal-title xml:lang="ru">Аграрная наука Евро-Северо-Востока</journal-title><trans-title-group xml:lang="en"><trans-title>Agricultural Science Euro-North-East</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2072-9081</issn><issn pub-type="epub">2500-1396</issn><publisher><publisher-name>FARC North-East</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.30766/2072-9081.2023.24.6.1007-1020</article-id><article-id custom-type="elpub" pub-id-type="custom">agronauka-1486</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ОРИГИНАЛЬНЫЕ СТАТЬИ: ХРАНЕНИЕ И ПЕРЕРАБОТКА СЕЛЬСКОХОЗЯЙСТВЕННОЙ ПРОДУКЦИИ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>ОRIGINAL SCIENTIFIC ARTICLES: STORAGE AND PROCESSING OF AGRICULTURAL PRODUCTION</subject></subj-group></article-categories><title-group><article-title>Комплексная биоконверсия вторичных продуктов переработки гороховой муки в кормовые дрожжи</article-title><trans-title-group xml:lang="en"><trans-title>Complex bioconversion of secondary products of processing pea flour into fodder yeast</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-7288-8569</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Колпакова</surname><given-names>В. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Kolpakova</surname><given-names>V. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Колпакова Валентина Васильевна, доктор техн. наук, профессор, главный научный сотрудник</p><p>ул. Некрасова, 11, Красково, Люберецкий район, Московская область, 140051</p></bio><bio xml:lang="en"><p>Valentina V. Kolpakova, DSc in Engineering, professor, chief researcher</p><p>11, Nekrasov Street, Kraskovo, Lyubertsy, Moscow region, 140051</p></bio><email xlink:type="simple">val-kolpakova@rambler.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-6315-7211</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Уланова</surname><given-names>Р. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Ulanova</surname><given-names>R. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Уланова Рузалия Владимировна, кандидат биол. наук, старший научный сотрудник, научный сотрудник</p><p>Ленинский пр-т, 33, г. Москва, 119071</p></bio><bio xml:lang="en"><p>Ruzaliya V. Ulanova, PhD in Biology, senior researcher, researcher</p><p>Leninskiy avenue, 33, Moscow, 119071</p></bio><email xlink:type="simple">info@fbras.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-2171-0522</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Куликов</surname><given-names>Д. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Kulikov</surname><given-names>D. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Куликов Денис Сергеевич, научный сотрудник</p><p>ул. Некрасова, 11, Красково, Люберецкий район, Московская область, 140051</p></bio><bio xml:lang="en"><p>Denis S. Kulikov, researcher</p><p>11, Nekrasov Street, Kraskovo, Lyubertsy, Moscow region, 140051</p></bio><email xlink:type="simple">vniik@arrisp.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-8393-9256</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Гулакова</surname><given-names>В. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Gulakova</surname><given-names>V. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Гулакова Валентина Андреева, научный сотрудник</p><p>ул. Некрасова, 11, Красково, Люберецкий район, Московская область, 140051</p></bio><bio xml:lang="en"><p>Valentina A. Gulakova, researcher</p><p>11, Nekrasov Street, Kraskovo, Lyubertsy, Moscow region, 140051</p></bio><email xlink:type="simple">vniik@arrisp.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Всероссийский научно-исследовательский институт крахмала и переработки крах малсодержащего сырья – филиал ФГБНУ «Федеральный исследовательский центр кар тофеля имени А. Г. Лорха»</institution><country>Россия</country></aff><aff xml:lang="en"><institution>All-Russian Research Institute of Starch and Starch-containing Raw Materials Processing – Branch of Russian Potato Research Centre</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>Институт микробиологии им. С. Н. Виноградского, ФГУ «Федеральный исследова тельский центр «Фундаментальные основы биотехнологии» Российской академии наук»</institution><country>Россия</country></aff><aff xml:lang="en"><institution>S. N. Vinogradsky Institute of Microbiology, Federal Research Centre «Fundamentals of Biotechnology» of the Russian Academy of Sciences</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2023</year></pub-date><pub-date pub-type="epub"><day>16</day><month>12</month><year>2023</year></pub-date><volume>24</volume><issue>6</issue><fpage>1007</fpage><lpage>1020</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Колпакова В.В., Уланова Р.В., Куликов Д.С., Гулакова В.А., 2023</copyright-statement><copyright-year>2023</copyright-year><copyright-holder xml:lang="ru">Колпакова В.В., Уланова Р.В., Куликов Д.С., Гулакова В.А.</copyright-holder><copyright-holder xml:lang="en">Kolpakova V.V., Ulanova R.V., Kulikov D.S., Gulakova V.A.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.agronauka-sv.ru/jour/article/view/1486">https://www.agronauka-sv.ru/jour/article/view/1486</self-uri><abstract><p>Цель исследований – разработка комплексной биоконверсии вторичных продуктов переработки (ВПП) гороховой муки: жидкой сыворотки и нерастворимого крахмалобелкового остатка (НКБО), образующихся при выделении пищевого белкового концентрата (БК), с использованием различных ферментных препаратов. Исследования проведены в 2021-2023 гг. Биоконверсия сыворотки и НКБО в кормовые дрожжи (КД) выполнена с ассоциацией культур S. cerevisiae 121 и G. candidum 977 (1:1). Создана математическая модель зависимости роста биомассы на сыворотке от технологических факторов, определены оптимальные параметры: рН, температура, количество посевного материала. В процессе синтеза биомассы из сыворотки микроорганизмы усваивали глюкозу, ксилозу, галактозу, фруктозу. Установлены параметры гидролиза НКБО и условия его совместной биоконверсии с сывороткой с теми же микроорганизмами: количество остатка, добавляемого к массе сыворотки, рН, температура, продолжительность процесса, давление. С гидролизованным при рН 1,8 НКБО количество белка в биомассе повышалось в 2,2 раза, восстанавливающих сахаров – в 6,1 раза по сравнению с исходной сывороткой. При этом усваивались глюкоза и мальтоза. Сухие КД содержали 51,09-61,68 % белка, 2-8 % липидов, золы 5-8 % на сухое вещество. Аминокислотный скор белков равнялся 90-247 %, соотношение насыщенных (23,5 %) и ненасыщенных жирных кислот (71,67 %) – 1:3, омега-6 жирные кислоты – 19,73 %, транс-изомеры – 5,0 %. Дрожжи богаты натрием, калием, кальцием, магнием, цинком. Массовая доля нуклеиновых кислот и тяжелых металлов в КД находилась в пределах нормы, перевариваемость in vitro составила 85,73-89,74 %. Данные указывали на целесообразность утилизации ВПП гороховой муки комплексной биоконверсией в КД высокого качества.</p></abstract><trans-abstract xml:lang="en"><p>The purpose of the research is to develop a comprehensive bioconversion of secondary processing products (SPP) of pea flour: liquid whey and insoluble starch-protein residue (ISPR), formed during the isolation of food protein concentrate (PC) using various enzyme preparations. The research was carried out in 2021-2023. The bioconversion of whey and ISPR into fodder yeast (FY) was performed with the association of cultures of S. cerevisiae 121 and G. candidum 977 (1:1). There was created a mathematical model of the dependence of biomass growth on whey on technological factors, and the optimal parameters were determined: pH, temperature, amount of inoculum. In the process of biomass synthesis microorganisms absorbed glucose, xylose, galactose, fructose from whey. The parameters of ISPR hydrolysis and the conditions for its joint bioconversion with whey with the same microorganisms were established: the amount of residue added to the mass of whey, pH, temperature, process duration, pressure. With insoluble starch-protein residue hydrolyzed at pH 1.8, the amount of protein in the biomass increased by 2.2 times, reducing sugars – by 6.1 times, compared with the original whey. At the same time, glucose and maltose were absorbed. Dry FYs contained 51.09-61.68 % protein, 2-8 % lipids, and ash content 5-8 % per dry matter. The amino acid score of proteins was 90-247 %, the ratio of saturated (23.5 %) and unsaturated fatty acids (71.67 %) – 1:3, omega-6 fatty acids – 19.73 %, trans-isomers – 5.0 %. Yeast is rich in sodium, potassium, calcium, magnesium, zinc. The mass fraction of nucleic acids and heavy metals in FYs were within the normal range, in vitro digestibility was 85.73-89.74 %. The data indicated the expediency of utilization of pea flour secondary processing products into high-quality FY by comprehensive bioconversion.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>вторичные продукты переработки</kwd><kwd>сыворотка</kwd><kwd>нерастворимый крахмалобелковый остаток</kwd></kwd-group><kwd-group xml:lang="en"><kwd>secondary processing products</kwd><kwd>whey</kwd><kwd>insoluble starch-protein residue</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Работа выполнена при поддержке Минобрнауки России в рамках Государственного задания ФГБНУ «Федеральный исследовательский центр картофеля имени А. Г. Лорха» (тема № FGGM-2022-0006). Авторы благодарят рецензентов за их вклад в экспертную оценку этой работы.</funding-statement><funding-statement xml:lang="en">The research was carried out under the support of the Ministry of Science and Higher Education of the Russian Federation within the state assignment of the Russian Potato Research Centre (theme No. FGGM-2022-0006). The authors thank the reviewers for their contribution to the peer review of this work.</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Xu J. C., Zhang M. L., He T., Luo H. J., Peng K. M., Huang X. F., Liu J. Application of de-lignified cellulose to enhance intracellular and extracellular lipid production from oleaginous yeast using acetic acid. Bioresource Technology. 2019;293:122032. DOI: https://doi.org/10.1016/j.biortech.2019.122032</mixed-citation><mixed-citation xml:lang="en">Xu J. C., Zhang M. 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