agronaukaАграрная наука Евро-Северо-ВостокаAgricultural Science Euro-North-East2072-90812500-1396FARC North-East10.30766/2072-9081.2024.25.2.236-250agronauka-1607Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИ: ЗООТЕХНИЯОRIGINAL SCIENTIFIC ARTICLES: ZOOTECHNYИдентификация генов-кандидатов, связанных с ростом и развитием овец из кроссбредной популяции, с использованием полногеномного поиска ассоциацийIdentification of candidate genes associated with growth and development of sheep from a crossbred population using genome-wide association studieshttps://orcid.org/0000-0002-5809-1262ДенисковаТ. Е.DeniskovaT. E.

Денискова Татьяна Евгеньевна, кандидат биол. наук, ведущий научный сотрудник группы генетики и геномики мелкого рогатого скота

пос. Дубровицы, д. 60, г. о. Подольск, Московская область, 142132

Tatiana E. Deniskova, PhD in Biological Science, leading researcher, Group for Genetic and Genomics of Small Ruminants

Dubrovitsy village, 60, Podolsk City District, Moscow Region, 142132

horarka@yandex.ruhttps://orcid.org/0000-0003-4830-6626КошкинаО. А.KoshkinaO. A.

Кошкина Ольга Андреевна, научный сотрудник группы генетики и геномики мелкого рогатого скота

пос. Дубровицы, д. 60, г. о. Подольск, Московская область, 142132

Olga A. Koshkina, researcher, Group for Genetic and Genomics of Small Ruminants

Dubrovitsy village, 60, Podolsk City District, Moscow Region, 142132

priemnaya-vij@mail.ruhttps://orcid.org/0000-0001-5130-677XПетровС. Н.PetrovS. N.

Петров Сергей Николаевич, кандидат биол. наук, старший научный лаборатория функциональной и эволюционной геномики животных

пос. Дубровицы, д. 60, г.о. Подольск, Московская область, 142132

Sergey N. Petrov, PhD in Biological Science, senior researcher, Laboratory of Functional and Evolutionary Genomics of Animals

Dubrovitsy village, 60, Podolsk City District, Moscow Region, 142132

priemnaya-vij@mail.ruhttps://orcid.org/0000-0002-1799-6014СермягинА. А.SermyaginA. A.

Сермягин Александр Александрович, кандидат с.-х. наук, ведущий научный сотрудник, зав. отделом популяционной генетики и генетических основ разведения животных

пос. Дубровицы, д. 60, г. о. Подольск, Московская область, 142132

Alexander A. Sermyagin, PhD in Agricultural Science, leading researcher, Head of Department of Population Genetics and Genetic Bases of Breeding

Dubrovitsy village, 60, Podolsk City District, Moscow Region, 142132

priemnaya-vij@mail.ruhttps://orcid.org/0000-0003-4017-6863ЗиновьеваН. А.ZinovievaN. A.

Зиновьева Наталия Анатольевна, доктор биол. наук, профессор, академик Российской академии наук, директор

пос. Дубровицы, д. 60, г. о. Подольск, Московская область, 142132

Natalia A. Zinovieva, DSc in Biological Science, professor, Academician of the Russian Academy of Sciences, Director

Dubrovitsy village, 60, Podolsk City District, Moscow Region, 142132

priemnaya-vij@mail.ruФГБНУ «Федеральный исследовательский центр животноводства – ВИЖ имени академика Л. К. Эрнста»РоссияFederal Research Center for Animal Husbandry named after Academy Member L. K. ErnstRussian Federation202424042024252236250Copyright © Денискова Т.Е., Кошкина О.А., Петров С.Н., Сермягин А.А., Зиновьева Н.А., 20242024Денискова Т.Е., Кошкина О.А., Петров С.Н., Сермягин А.А., Зиновьева Н.А.Deniskova T.E., Koshkina O.A., Petrov S.N., Sermyagin A.A., Zinovieva N.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.agronauka-sv.ru/jour/article/view/1607

В статье представлены результаты поиска полногеномных ассоциаций с фенотипическими показателями, характеризующими рост и развитие овец из кроссбредной популяции, полученной от скрещивания овец романовской породы и F1 гибридных баранов (романовская овца х катадин). База фенотипов включала десять промеров туловища (высота в холке, высота в крестце, высота спины, глубина груди, ширина груди за лопатками, ширина в маклоках, длина туловища, косая длина туловища, обхват груди, обхват пясти), зафиксированных в возрасте 6 дней, 3, 6 и 9 месяцев. Генотипирование овец проводили с помощью ДНК-чипов высокой плотности, содержащих около 600 000 SNP-маркеров. Полногеномные ассоциативные исследования (genome-wide association study, GWAS) выполнены путем регрессионного анализа в программе STATISTICA 10. Поиск генов-кандидатов, локализованных в области SNP, был осуществлен посредством Ensembl genome browser 110. Проведен анализ совпадений, идентифицированных SNP с известными локусами количественных признаков (QTL), включенными в базу данных Sheep Quantitative Trait Locus Database. Выявлены SNP, достоверно ассоциированные с изучаемыми фенотипическими показателями, располагающиеся внутри QTL, среди которых наиболее часто встречались категории «Масса туши», «Масса мышечной ткани в туше», «Живая масса» и «Масса костей». Установлено, что SNP, достоверно ассоциированные с экстерьерными показателями, были локализованы внутри или в непосредственной близости от 64 генов. Обнаружены потенциальные кандидаты, регулирующие рост мышечной (FOXO3, PRKAG3, MYOZ2 и ANKRD1) и хрящевой тканей (FGF12) и участвующие в критических для роста ягнят метаболических процессах (CLDN, ALB и MRC1). Наряду с известными у овец функциональными кандидатами (CAST и SCD5) выявлены гены, не описанные ранее у овец, но регулирующие процессы роста и развития у других видов сельскохозяйственных животных, в том числе гены RAB28, PRKAG3 и FOXO3. Идентифицированные SNP могут быть рекомендованы для включения в программы маркер-ориентированной селекции в овцеводстве.

The article presents the results of a search for genome-wide associations with phenotypic traits characterizing the growth and development of sheep from a crossbred population obtained from crossing Romanov sheep and F1 hybrid rams (Romanov sheep x Katahdin). The phenotype database included ten body measurements (withers height, sacral height, back height, chest depth, chest width, ischial tuberosity width, body length, oblique body length, chest girth, pastern girth) recorded at the age of 6 days, 3, 6 and 9 months. Genotyping of sheep was carried out using high-density DNA chips containing about 600,000 SNP markers. Genome-wide association studies (GWAS) were performed using regression analysis in the STATISTICA 10 program. The search for candidate genes localized in the SNP region was performed using Ensembl genome browser 110. There was carried out an analysis of the matches of the identified SNPs with known quantitative trait loci (QTLs) described in the Sheep Quantitative Trait Locus Database. There were found SNPs that were significantly associated with the studied phenotypic traits overlapped with the QTLs, among which the most common categories were “Body weight (slaughter)”, “Muscle weight in carcass”, “Body weight (live)” and “Bone weight in carcass”. There has been established that SNPs significantly associated with exterior traits were localized within or in the immediate vicinity of 64 genes. There were found potential candidates regulating the growth of muscle (FOXO3, PRKAG3, MYOZ2, and ANKRD1) and cartilage tissues FGF12) and involved in metabolic processes, which were critical for the growth of lambs (CLDN, ALB, and MRC1). Along with the known in sheep functional candidates (CAST and SCD5) , there were identified genes that were not previously described in sheep, but regulated growth and development processes in other livestock species including genes RAB28, PRKAG3 and  FOXO3. The identified SNPs can be recommended for inclusion in marker-guided selection programs in sheep breeding.

SNPGWASQTLэкстерьерные показателиресурсная популяцияДНК-чипыSNPGWASQTLexterior traitsresource populationDNA chipsработа выполнена при поддержке Российского научного фонда, проект № 21-66-00007. Для проведения исследований использовали оборудование ЦКП «Биоресурсы и биоинженерия сельскохозяйственных животных» ФГБНУ ФИЦ ВИЖ им. Л.К. Эрнста. Авторы благодарят рецензентов за их вклад в экспертную оценку этой работы.The study was supported by the Russian Science Foundation, Project No. 21-66-00007. During the research, the equipment of the Center for Biological Resources and Bioengineering of Agricultural Animals (L. K. Ernst Federal Research Center for Animal Husbandry). The authors thank the reviewers for their contribution to the expert evaluation of this work.ReferencesСамусенко Л. Д. Стратегические направления в развитии продукции овцеводства. Вестник сельского развития и социальной политики. 2021;1(29):6–8. 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The authors declare that there are no conflicts of interest present.