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<article article-type="review-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">agronauka</journal-id><journal-title-group><journal-title xml:lang="ru">Аграрная наука Евро-Северо-Востока</journal-title><trans-title-group xml:lang="en"><trans-title>Agricultural Science Euro-North-East</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2072-9081</issn><issn pub-type="epub">2500-1396</issn><publisher><publisher-name>FARC North-East</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.30766/2072-9081.2024.25.3.319-329</article-id><article-id custom-type="elpub" pub-id-type="custom">agronauka-1661</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ОБЗОРНЫЕ СТАТЬИ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>REVIEW</subject></subj-group></article-categories><title-group><article-title>Приемы повышения эффективности микроклонального размножения картофеля (обзор)</article-title><trans-title-group xml:lang="en"><trans-title>Techniques for increasing the efficiency of microclonal propagation of potatoes (review)</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-2435-2089</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Смирнова</surname><given-names>Ю. Д.</given-names></name><name name-style="western" xml:lang="en"><surname>Smirnova</surname><given-names>Yu. D.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Смирнова Юлия Дмитриевна, кандидат биол. наук,  заместитель директора филиала по науке, Всероссийский научно-исследовательский институт  мелиорированных земель – филиал</p><p>д.27, п. Эммаусс, Калининский р-н, Тверская обл.,  170530</p></bio><bio xml:lang="en"><p>Yulia D. Smirnova, PhD in Biological Science, Deputy Director of the Branch for Science, the Department of  Reclamation Agriculture, All-Russian Research Institute of Reclaimed Lands – Branch</p><p>27, Emmauss village, Kalininsky district, Tver region, 170530</p></bio><email xlink:type="simple">2016vniimz-noo@list.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-2754-0053</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Подолян</surname><given-names>Е. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Podolian</surname><given-names>E. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Подолян Елена Александровна, кандидат с.-х. наук,  младший научный сотрудник, Всероссийский  научно-исследовательский институт мелиорированных земель – филиал</p><p>д.27, п. Эммаусс, Калининский р-н, Тверская обл., 170530</p></bio><bio xml:lang="en"><p>Elena A. Podolian, PhD in Agricultural Science, junior researcher, the Department of Reclamation Agriculture,  All-Russian Research Institute of Reclaimed Lands –  Branch</p><p>27, Emmauss village, Kalininsky district, Tver region, 170530</p></bio><email xlink:type="simple">2016vniimz-noo@list.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">ФГБНУ Федеральный исследовательский центр «Почвенный институт им. В. В. Докучаева»<country>Россия</country></aff><aff xml:lang="en">Federal Research Center V. V. Dokuchaev Soil Institute<country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2024</year></pub-date><pub-date pub-type="epub"><day>26</day><month>06</month><year>2024</year></pub-date><volume>25</volume><issue>3</issue><fpage>319</fpage><lpage>329</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Смирнова Ю.Д., Подолян Е.А., 2024</copyright-statement><copyright-year>2024</copyright-year><copyright-holder xml:lang="ru">Смирнова Ю.Д., Подолян Е.А.</copyright-holder><copyright-holder xml:lang="en">Smirnova Y.D., Podolian E.A.</copyright-holder><license license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.agronauka-sv.ru/jour/article/view/1661">https://www.agronauka-sv.ru/jour/article/view/1661</self-uri><abstract><p>Картофель (Solanum tuberosum L.) является важной сельскохозяйственной культурой, обеспечивающей продовольственную безопасность многих стран. Одним из главных факторов, способствующих получению высоких урожаев картофеля является использование качественного семенного материала. Применение метода культуры тканей для размножения растений картофеля является наиболее значимым. В работе проанализированы источники отечественной и зарубежной литературы, представляющие результаты исследований, связанных с изучением и совершенствованием приемов размножения in vitro и выращивания in vivo для получения исходного материала картофеля. Публикации выбраны из открытых источников за последние 10 лет. Рассмотрено влияние различных регуляторов роста (цитокины, ауксины, гибберелины, бензихол, этихол, гуминовые кислоты), минерального состава питательной среды Мурасиге-Скуга (MS), физических факторов на биомассу, длину побегов и корней, количество узлов и укоренение эксплантов в условиях in vitro. При этом оптимальные параметры могут варьировать в зависимости от сорта картофеля. Отдельно проанализированы факторы (изменение состава среды, физических показателей), оказывающие влияние на увеличение образования числа микроклубней в культуре in vitro. Данный метод получения оздоровленного материала является наиболее перспективным. Среди приёмов повышения адаптации пробирочных растений in vivo рассмотрены предварительное замачивание перед высаживанием в грунт пробирочных растений картофеля и прикорневая обработка гуминовыми препаратами, фитоиммуномодуляторами, а также различные варианты укрытия растений ex vitro. Показано, что размножение пробирочных растений на установках аэропонного и гидропонного типа позволяет минимизировать воздействие внешних факторов, в том числе попадание инфекционных заболеваний.</p></abstract><trans-abstract xml:lang="en"><p>Potato (Solanum tuberosum L.) is an important crop that ensures food security in many countries. One of the main factors ensuring high potato yields is the use of high-quality seed material. The application of tissue culture method for propagation of potato plants is the most significant. The work analyzes sources of domestic and foreign literature, presenting the results of research related to the study and improvement of in vitro propagation and in vivo cultivation techniques for obtaining potato source material. Publications were selected from open sources over the past 10 years. The influence of various growth regulators (cytokines, auxins, gibberellins, benzichol, etihol, humic acids), the mineral composition of the Murashige-Skoog (MS) nutrient medium, and physical factors on biomass, the length of shoots and roots, the number of nodes and rooting of explants under in vitro conditions is considered. At the same time, the optimal parameters may vary depending on the potato variety. Factors (changes in the composition of the environment, physical indicators) that influence the increase in the formation of the number of microtubers in in vitro culture are considered separately. This method of obtaining healthy material is the most promising. Among the methods for increasing the adaptation of test tube plants in vivo, preliminary soaking before planting test tube potato plants in the soil and root treatment with humic preparations, phytoimmunomodulators, as well as various options for covering plants ex vitro are considered. It has been shown that the propagation of test tube plants in aeroponic and hydroponic installations allows minimizing the impact of external factors, including the introduction of infectious diseases.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>семеноводство картофеля</kwd><kwd>культура in vitro</kwd><kwd>адаптация in vivo</kwd><kwd>Solanum tuberosum L.</kwd></kwd-group><kwd-group xml:lang="en"><kwd>potato seed production</kwd><kwd>in vitro culture</kwd><kwd>in vivo adaptation</kwd><kwd>Solanum tuberosum L.</kwd></kwd-group><funding-group xml:lang="ru"><funding-statement>работа выполнена при поддержке Минобрнауки РФ в рамках Государственного задания ФГБНУ Федеральный исследовательский центр «Почвенный институт имени В. В. Докучаева» (тема № FGUR-2022-0007).</funding-statement></funding-group><funding-group xml:lang="en"><funding-statement>the research was carried out under the support of the Ministry of Science and Higher Education of the Russian Federation within the state assignment of Federal Research Center V. V. Dokuchaev Soil Science Institute (theme No. FGUR-2022-0007).</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Гавриленко Т. А., Ермишин А. П. Межвидовая гибридизация картофеля: теоретические и прикладные аспекты. Вавиловский журнал генетики и селекции. 2017;21(1):16–29. DOI: http://doi.org/10.18699/VJ17.220EDN: XYEBBV</mixed-citation><mixed-citation xml:lang="en">Gavrilenko T. A., Ermishin A. P. Interspecific hybridization of potato: theoretical and applied aspects. 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