agronaukaАграрная наука Евро-Северо-ВостокаAgricultural Science Euro-North-East2072-90812500-1396FARC North-East10.30766/2072-9081.2025.26.4.906-916agronauka-2160Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИ: ВЕТЕРИНАРНАЯ МЕДИЦИНАОRIGINAL SCIENTIFIC ARTICLES: VETERINARY MEDICINEОднократная иммунизация аттенуированным штаммом «Волгоград/14с» вируса АЧС не защищает поросят от заражения гомологичным штаммом «Ставрополь 01/08»Single immunization with the attenuated strain “Volgograd/14c” of the ASF virus does not protect piglets from infection with the homologous strain “Stavropol 01/08”https://orcid.org/0000-0002-8324-3256ВласовМ. Е.VlasovM. E.

Власов Михаил Евгеньевич, кандидат вет. наук, заведующий сектором,

ул. Академика Бакулова, стр.1, пос. Вольгинский, Петушинский р-н., Владимирская обл., 601125

Mikhail E. Vlasov, PhD in Veterinary Science, Head of the Sector,

Academician Bakulov St., Volginsky, Petushinsky District, Vladimir Region, 601125

info@ficvim.ruhttps://orcid.org/0000-0002-6793-0195КудряшовД. А.KudryashovD. A.

Кудряшов Дмитрий Андреевич, начальник группы,

ул. Академика Бакулова, стр.1, пос. Вольгинский, Петушинский р-н., Владимирская обл., 601125

Dmitriy A. Kudryashov, Head of the Group, 

Academician Bakulov St., Volginsky, Petushinsky District, Vladimir Region, 601125

info@ficvim.ruhttps://orcid.org/0000-0002-5947-9402СиндряковаИ. П.SindryakovaI. P.

Синдрякова Ирина Петровна, кандидат биол. наук, заведующий лабораторией,

ул. Академика Бакулова, стр.1, пос. Вольгинский, Петушинский р-н., Владимирская обл., 601125

Irina P. Sindryakova, PhD in Biology, Head of the Laboratory,

Academician Bakulov St., Volginsky, Petushinsky District, Vladimir Region, 601125

info@ficvim.ruhttps://orcid.org/0000-0001-9730-3179МоргуновС. Ю.MorgunovS. Yu.

Моргунов Сергей Юрьевич, кандидат биол. наук, начальник группы,

ул. Академика Бакулова, стр.1, пос. Вольгинский, Петушинский р-н., Владимирская обл., 601125

Sergey Yu. Morgunov, PhD in Biology, Head of the Group, 

Academician Bakulov St., Volginsky, Petushinsky District, Vladimir Region, 601125

info@ficvim.ruhttps://orcid.org/0000-0002-5821-8980ТитовИ. А.TitovI. A.

Титов Илья Андреевич, кандидат биол. наук, заведующий лабораторией,

ул. Академика Бакулова, стр.1, пос. Вольгинский, Петушинский р-н., Владимирская обл., 601125

Iliy A. Titov, PhD in Biology, Head of the Laboratory,

Academician Bakulov St., Volginsky, Petushinsky District, Vladimir Region, 601125

info@ficvim.ruФГБНУ «Федеральный исследовательский центр вирусологии и микробиологии»РоссияFederal Research Center for Virology and MicrobiologyRussian Federation202506092025264906916Copyright © Власов М.Е., Кудряшов Д.А., Синдрякова И.П., Моргунов С.Ю., Титов И.А., 20252025Власов М.Е., Кудряшов Д.А., Синдрякова И.П., Моргунов С.Ю., Титов И.А.Vlasov M.E., Kudryashov D.A., Sindryakova I.P., Morgunov S.Y., Titov I.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.agronauka-sv.ru/jour/article/view/2160

После заноса вируса АЧС в 2007 году в Грузию болезнь распространилась по многим странам Европы и Азии. С момента заноса АЧС регистрировали в 42 странах Евразийского континента. Для искоренения болезни используют жесткие противоэпизоотические мероприятия, включающие в себя политику стемпинг аут, запрет на ввоз и вывоз всех видов животных, запрет на торговлю и другие мероприятия. С целью профилактики заболевания были разработаны и экспериментально проверены кандидатные инактивированные, живые ослабленные, субъединичные, векторные и ДНК-вакцины, испытания которых во многих случаях не увенчались успехом. Цель работы  – изучить иммунобиологические и протективные свойства аттенуированного штамма вируса АЧС «Волгоград/14с» против заражения гомологичным вирулентным штаммом «Ставрополь 01/08» in vivo. Однократное внутримышечное введение аттенуированного штамма  «Волгоград/14с» в дозе 103,0 ГАЕ50/см3 шести подсвинкам вызывало 1–4-дневную гипертермию (40,0–40,8 oC) без развития других клинических признаков. Виремию в крови инокулированных подсвинков выявляли с 3–7-го дня в титрах 2,0-3,0 lg ГАЕ50/см3 с максимальным накоплением 3,75 lg ГАЕ50/см3 на 10-й день, снижением на 14–21-й день до 1,75–2,75 lg ГАЕ50/см3 и утрате инфекционности к 28-му дню. С 10-го дня в сыворотке крови привитых животных обнаруживали вирусоспецифические антитела к вирусу АЧС. Контрольное заражение гомологичным вирулентным штаммом «Ставрополь 01/08» вызывало развитие клинических признаков АЧС и рост виремии в крови до 6,0–7,5 lg ГАЕ50/см3 у всех поросят опытной и контрольной групп. Болезнь протекала сверхостро, остро, подостро и хронически с гибелью всех контрольных и 5 из 6 подопытных подсвинков с характерными для АЧС клиническими признаками и патологоанатомическими изменениями.

After the introduction of the ASF virus to Georgia in 2007, the disease spread to many countries in Europe and Asia. In present time, ASF has been registered in 42 countries of the Eurasian continent. Strict anti-epizootic measures are used to eradicate the disease, including stamping out policies, bans on import and export of all types of animals, trade bans, etc. In order to prevent the disease, different vaccine candidates have been developed, such as inactivated, live attenuated, subunit, vector and DNA vaccines. Unfortunately, in many cases, trials of these vaccine candidates have been unsuccessful. The aim of the work was to study the immunobiological and protective properties of the attenuated ASF virus strain “Volgograd/14c” against infection with the homologous virulent strain “Stavropol 01/08” in vivo. A single intramuscular inoculation of the attenuated strain “Volgograd/14s” at a dose of 103,0HAU50см3 to six pigs caused 1–4 day hyperthermia (40.0-40.8oC) without the development of other clinical signs. Viremia in the blood of inoculated pigs was detected from day 3–7 in titers of 2.0-3.0 lg HAU50см3, with a maximum accumulation of 3.75 lg HAU50см3 on day 10, a decrease on days 14–21 to 1.75–2.75 lg HAU50см3 and loss of infectivity by day 28. Virus-specific antibodies to the ASF virus were detected in the serum of vaccinated animals from day 10. Challenge with the homologous virulent strain “Stavropol 01/08” caused the development of clinical signs and the growth of viremia in the blood to 6.0–7.5 lg HAU50см3 in all pigs of the experimental and control groups. The course of the disease was hyperacute, acute, subacute and chronic with the death of all control animals and 5 of 6 experimental pigs with clinical signs and pathological changes characteristic to ASF.

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The authors declare that there are no conflicts of interest present.