agronaukaАграрная наука Евро-Северо-ВостокаAgricultural Science Euro-North-East2072-90812500-1396FARC North-East10.30766/2072-9081.2025.26.6.1375-1401agronauka-2294Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИ: ЗООТЕХНИЯОRIGINAL SCIENTIFIC ARTICLES: ZOOTECHNYИдентификация генов-кандидатов, связанных с экстерьером крупного рогатого скота голштинизированной черно-пестрой породы на основе GWAS-анализаIdentification of candidate genes associated with conformation in Holsteinized Black and White cattle based on GWAS analysishttps://orcid.org/0000-0003-0487-4576НедашковскийИ. С.NedashkovskyI. S.

Недашковский Игорь Сергеевич, кандидат биол. наук, старший научный сотрудник, зав. отделом национального каталога Национального центра генетических ресурсов с.-х. животных

пос. Дубровицы, д. 60, г.о. Подольск, Московская область, 142132

Igor S. Nedashkovsky, PhD in Biological Science, senior researcher, Head of the National Catalog Department of the National Center for Genetic Resources of Farm Animals

Dubrovitsy village, 60, Podolsk City District, Moscow Region, 142132

nedashkovsky_is@mail.ruhttps://orcid.org/0000-0003-4877-0883КонтэА. Ф.KonteA. F.

Контэ Александр Федорович, кандидат с.-х. наук, старший научный сотрудник отдела популяционной генетики и генетических основ разведения животных

пос. Дубровицы, д. 60, г.о. Подольск, Московская область, 142132

Aleksandr F. Konte, PhD in Agricultural Science, senior researcher, the Department of Population Genetics and Genetic Basis of Animal Breeding

Dubrovitsy village, 60, Podolsk City District, Moscow Region, 142132

vijinfo@yandex.ruhttps://orcid.org/0000-0002-1799-6014СермягинА. А.SermyaginA. A.

Сермягин Александр Александрович, кандидат с.-х. наук, директор 

д. 55а, Московское шоссе, г. Пушкин, г. Санкт-Петербург, 196601

Aleksandr A. Sermyagin, PhD in Agricultural Science, director

Moskovskoe Shosse, 55a, Pushkin, St. Petersburg, 196601

spbvniigen@mail.ruhttps://orcid.org/0000-0002-3977-8552КольцовД. Н.KoltsovD. N.

Кольцов Дмитрий Николаевич, доктор биол. наук, директор, обособленное подразделение Смоленский НИИСХ 

д. 21, ул. Нахимова, г. Смоленск, 214025

Dmitry N. Koltsov, DCs in Biological Science, director, the separate division Smolensk Research Institute of Agriculture

st. Nakhimova 21 Smolensk, 214025

info.sml@fnclk.ruhttps://orcid.org/0000-0002-2080-0182ВолковаВ. В.VolkovaV. V.

Волкова Валерия Владимировна, кандидат биол. наук, старший научный сотрудник лаборатории ДНК-технологий в животноводстве

пос. Дубровицы, д. 60, г.о. Подольск, Московская область, 142132

Valeria V. Volkova, PhD in Biological Science, senior researcher, the Laboratory of DNA Technologies in Animal Husbandry

Dubrovitsy village, 60, Podolsk City District, Moscow Region, 142132

vijinfo@yandex.ruФГБНУ «Федеральный исследовательский центр животноводства – ВИЖ имени академика Л. К. Эрнста»РоссияFederal Research Center for Animal Husbandry named after Academy Member L. K. ErnstRussian FederationВсероссийский научно-исследовательский институт генетики и разведения сельскохозяйственных животных – филиал ФГБНУ «Федеральный исследовательский центр животноводства – ВИЖ имени академика Л. К. Эрнста»РоссияRussian Research Institute of Farm Animal Genetics and Breeding – Branch of the L. K. Ernst Federal Research Center for Animal HusbandryRussian FederationФГБНУ Федеральный научный центр лубяных культурРоссияFederal Research Center for Bast Fiber CropsRussian Federation20250901202626613751401Copyright © Недашковский И.С., Контэ А.Ф., Сермягин А.А., Кольцов Д.Н., Волкова В.В., 20252025Недашковский И.С., Контэ А.Ф., Сермягин А.А., Кольцов Д.Н., Волкова В.В.Nedashkovsky I.S., Konte A.F., Sermyagin A.A., Koltsov D.N., Volkova V.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.agronauka-sv.ru/jour/article/view/2294

Проведено исследование, направленное на поиск полногеномных ассоциаций с фенотипическими признаками экстерьера у голштинизированной черно-пестрой породы крупного рогатого скота (356 быков для 42247 дочерей). В работе использована методика Союза «Мосплем», которая включает 4 признака по системе «А» и 17 признаков по системе «Б». Генотипирование проводили на основе биочипа Illumina BovineSNP50 (54609 SNPs). Поиск геновкандидатов, локализованных в области идентифицированных SNP осуществляли в базе данных NCBI по сборке генома Bos_taurus_UMD_3.1.1. Для поиска QTL и регионов под давлением отбора использовали базу данных CattleQTLdb. По системе «А» в области данных, достоверно значимых SNP идентифицировано 26 геновкандидатов, ассоциированных с изучаемыми признаками экстерьера. По системе «Б» выявлено 22 SNP, ассоциированных с признаками экстерьера. На хромосомах 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 14, 15, 16, 17, 19, 20, 21, 22, 25, 26, 28 локализованы гены: ADCY5, FRYL, GOLGB1, ILDR1, ITGB5, MAPK10, SEC22A, STXBP5L, ZP2, ALDH1A2, ERGIC1, KCND2, PAPPA2, PPFIA2, PRDM16, RABGAP1L, SECISBP2, SMYD3, ANO3, DPP10, HPSE2, MAML3, PRKCE, SLCO3A1, ANTXR1, KCTD2, NCOA1, NRG3, TIGAR, VAV3, XYLT1, PRR5L, BFSP1, CDH4, HECW1, MAPKAP1, SPOCK1, SRP68, SYNJ2, DAB1, EPS8, PCDH15, PTPRR, TRPM7, TTYH2, UBR1, USP32, ANGPT1, ITCH, OSBPL10, RAI1, RGS22, SLA2, ZFHX4, BCL9, GFRA2, SLC25A12, CNNM2, IGFBP7, KALRN, MACROD2, PCSK5, UCK2, CNKSR3, CUL3, CYP27A1, RHPN1, TSNARE1, EBF1, PTCH1. Выявленные гены сопряжены с локусами количественных признаков, ассоциированных с различными показателями, которые согласуются с ранее проведенными исследованиями других авторов. Достоверность значимых ассоциаций по точечным мутациям находилась в пределах p<1,80E-08–0,0001474. Такой инструмент генетического исследования как GWAS-анализ позволяет эффективнее планировать получение особей, которые бы отвечали экономическим потребностям молочного скотоводства.

There was conducted a study aimed at identifying genome-wide associations with phenotypic exterior traits in Holsteinized Black-and-White cattle (n=356 heads for 42,247 daughters). During the research there has been used the methodology of the Mosplem Union which includes 4 traits according to system A and 17 traits according to system B. Genotyping was performed on the basis of the Illumina BovineSNP50 (54609 SNPs). The search for candidate genes localized in the region of identified SNPs was performed in the NCBI database for the Bos_taurus_UMD_3.1.1 genome assembly. The CattleQTLdb database was used to search for QTL and areas under selection pressure. According to system "A" in the data region of significantly significant SNPs, 26 candidate genes associated with the studied exterior traits were identified. According to system "B", 22 SNPs associated with exterior traits were identified. The following genes are localized on chromosomes 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 14, 15, 16, 17, 19, 20, 21, 22, 25, 26, 28: ADCY5, FRYL, GOLGB1, ILDR1, ITGB5, MAPK10, SEC22A, STXBP5L, ZP2, ALDH1A2, ERGIC1, KCND2, PAPPA2, PPFIA2, PRDM16, RABGAP1L, SECISBP2, SMYD3, ANO3, DPP10, HPSE2, MAML3, PRKCE, SLCO3A1, ANTXR1, KCTD2, NCOA1, NRG3, TIGAR, VAV3, XYLT1, PRR5L, BFSP1, CDH4, HECW1, MAPKAP1, SPOCK1, SRP68, SYNJ2, DAB1, EPS8, PCDH15, PTPRR, TRPM7, TTYH2, UBR1, USP32, ANGPT1, ITCH, OSBPL10, RAI1, RGS22, SLA2, ZFHX4, BCL9, GFRA2, SLC25A12, CNNM2, IGFBP7, KALRN, MACROD2, PCSK5, UCK2, CNKSR3, CUL3, CYP27A1, RHPN1, TSNARE1, EBF1, PTCH1. The identified genes are associated with quantitative trait loci associated with various indicators that are consistent with those previously annotated by other researchers. The reliability of significant associations for point mutations was within the range of p<1.80E-08 – 0.0001474. Such a tool of genetic research as GWAS analysis allows more efficient planning of obtaining individuals that would meet the economic needs of dairy cattle breeding.

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The authors declare that there are no conflicts of interest present.