agronaukaАграрная наука Евро-Северо-ВостокаAgricultural Science Euro-North-East2072-90812500-1396FARC North-East10.30766/2072-9081.2018.65.4.98-102agronauka-244Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИ: ЗООТЕХНИЯОRIGINAL SCIENTIFIC ARTICLES: ZOOTECHNYПолиморфизм SNP rs80867243, rs81379421 и rs81236069 у свиней материнских породSNP polymorphism rs80867243, rs81379421 and rs81236069 in pigs of maternal breedsГетманцеваЛ. В.GetmantsevaL. V.ilonaluba@mail.ruКолосоваМ. А.KolosovaM. A.m.leonovaa@mail.ruБакоевС. Ю.BakoevS. Yu.siroj1@yandex.ruФорнараМ. С.FornaraM. S.margaretfornara@gmail.comБардуковН. В.BardukovN. V.bardukv-nikolajj@mail.ruКарпушкинаТ. В.KarpushkinaT. B.tati.kriz@gmail.comФГБНУ ФНЦ Всероссийский институт животноводства им. Л.К. ЭрнстаРоссияFederal Science Center for Animal Husbandry named after Academy Member L.K. ErnstRussian FederationФГБОУ ВО «Донской государственный аграрный университет»РоссияFederal State Budgetary Educational Institution of Higher Education DON STATE AGRARIAN UNIVERSITYRussian Federation20182808201865498102Copyright © Гетманцева Л.В., Колосова М.А., Бакоев С.Ю., Форнара М.С., Бардуков Н.В., Карпушкина Т.В., 20182018Гетманцева Л.В., Колосова М.А., Бакоев С.Ю., Форнара М.С., Бардуков Н.В., Карпушкина Т.В.Getmantseva L.V., Kolosova M.A., Bakoev S.Y., Fornara M.S., Bardukov N.V., Karpushkina T.B.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.agronauka-sv.ru/jour/article/view/244

Полногеномные ассоциативные исследования (GWAS) находят все большее применение при изучении генетической архитектуры репродуктивных признаков свиней. По результатам этих исследований установлено 47 SNPs, связанных с многоплодием свиноматок европейских пород (крупная белая, йоркшир, ландрас), которые представлены в базе данных PigQTLdb. Цель данной работы - разработать методику тестирования SNPs rs80867243 (SSC5), rs81379421 (SSC3), rs81236069 (SSC10) методом ПЦР-ПДРФ и оценить их полиморфизм у свиней материнских пород, разводимых в условиях племенных хозяйств РФ. Исследования проводили на свиньях породы крупная белая (n = 60 гол.) и ландрас (n = 63 гол.). Позиции SNP определяли по последней сборке генома свиней (Sus scrofa 11.1) в базе данных Ensembl. Олигонуклеотидные праймеры и эндонуклеазы рестрикции для идентификации SNP методом ПЦР-ПДРФ подбирали с помощью программ Primer-BLAST и NEBcutter V2.0 соответственно. По результатам молекулярно-генетических исследований установлено, что свиньи породы ландрас полиморфны по всем исследуемым SNPs. Частоты аллелей A и G по SNPs rs80867243, rs81379421 и rs81236069 составили 0,81 и 0,19; 0,43 и 0,57; 0,21 и 0,79 соответственно. У свиней крупной белой породы установлено наличие полиморфизма по SNPs rs81379421 и rs81236069, частоты аллелей A и G составили 0,07 и 0,93; 0,30 и 0,70 соответственно. В работе представлены методики тестирования SNPs rs80867243, rs81379421 и rs81236069, которые могут быть использованы в дальнейших исследованиях на поголовье свиней, разводимых в племенных хозяйствах РФ, а также при изучении ассоциативных связей SNPs с воспроизводительными качествами.

Genome-Wide Association Studies (GWAS) are increasingly used in the investigation of the genetic architecture of the reproductive traits of pigs. Based on the results of these studies, 47 SNPs associated with the Total number born alive (NBA) of European breeds (Large White, Yorkshire, Landrace) were identified, which are presented in the PigQTLdb. The aim of this work is to develop a procedure for testing SNPs rs80867243 (SSC5), rs81379421 (SSC3), rs81236069 (SSC10) by PCR-RFLP and to evaluate their polymorphism in maternal pigs bred in the conditions of the Russian breeding industry. The research was carried out in pigs of Large White breed (n = 60) and Landrace (n = 63). SNP positions were determined from the last assembly of the pig genome (Sus scrofa 11.1) in the Ensembl database. The primers and restriction endonucleases for identification of SNP by PCR-RFLP were selected using Primer-BLAST and NEBcutter V2.0 programs, respectively. According to the results of molecular genetic studies, Landrace pigs are polymorphic in all investigated SNPs. The allele frequencies A and G for SNPs rs80867243, rs81379421 and rs81236069 were 0.81 and 0.19; 0.43 and 0.57; 0.21 and 0.79, respectively. In Large White pigs polymorphism was established for SNPs rs81379421 and rs81236069, the frequencies of alleles A and G were 0.07 and 0.93; 0.30 and 0.70, respectively. The methods of testing SNPs rs80867243, rs81379421 and rs81236069, which can be used in further studies on the pigs bred in breeding farms of the Russian Federation as well as in the study of associative connections of SNPs with reproductive traits are presented in the article.

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The authors declare that there are no conflicts of interest present.