Current state and prospects of protoplast technology and potato somatic hybridization (review)
https://doi.org/10.30766/2072-9081.2023.24.1.7-19
Abstract
Wild Solanum species have often been used as sources of important agricultural traits, including resistance to various diseases, pests, and abiotic factors. However, their large-scale use in potato breeding is limited by complex barriers of sexual incompatibility with Solanum tuberosum. Fusion of protoplasts enzymatically isolated from somatic cells is one of the approaches to overcoming sexual incompatibility. The diverse nuclear and cytoplasmic traits exhibited by potato somatic hybrids provide new genetic material for breeding programs, which is confirmed by the creation of a large number of somatic hybrids of cultivated potatoes with wild Solanum species. The research in development of somatic potato hybrids by means of protoplast fusion has been carried out for more than 40 years already. In this review, the prospects for the use of this technology in modern potato breeding are considered. Genomic, transcriptomic, and proteomic studies provide further insight into the fundamental processes underlying the somatic hybrids formation, such as cell wall formation, chromosomal rearrangements in fusion products, regeneration, and also make a significant contribution to understanding the processes of genome stabilization. Improvement in the methods of molecular screening of both genome and cytoplasm also contributes to the expansion of the field of application of somatic hybrids in breeding. Finally, it has been shown that somatic hybridization promotes the introgression of important agricultural traits, primarily resistance to pathogens.
Supporting agencies: the research was carried out under the support of the Ministry of Science and Higher Education of the Russian Federation within the state assignment of the Russian Potato Research Centre (theme No. FGGM -2022-0002). The authors thank the reviewers for their contribution to the expert evaluation of this work.
About the Authors
O. B. Polivanova
Russian Potato Research Centre
Russian Federation
Russian Federation
Oksana B. Polivanova, researcher, the Laboratory of Cell and Genomic Technologies
st. Lorkh, 23 B, Kraskovo, Lyubertsy, Moscow region,140051
A. S. Egorova
Russian Potato Research Centre
Russian Federation
Russian Federation
Anna S. Egorova, junior researcher, the Laboratory of Cell and Genomic Technologies
st. Lorkh, 23 B, Kraskovo, Lyubertsy, Moscow region,140051
A. B. Sivolapova
Russian Potato Research Centre
Russian Federation
Russian Federation
Anastasia B. Sivolapova, junior researcher, the Laboratory of Cell and Genomic Technologies
st. Lorkh, 23 B, Kraskovo, Lyubertsy, Moscow region,140051
S. V. Goryunova
Russian Potato Research Centre
Russian Federation
Russian Federation
Svetlana V. Goryunova, leading researcher, the Laboratory of Cell and Genomic Technologies
st. Lorkh, 23 B, Kraskovo, Lyubertsy, Moscow region,140051
References
1. Nagata T., Takebe I. Cell wall regeneration and cell division in isolated tobacco mesophyll protoplasts. Planta. 1970;92:301-308. DOI: https://doi.org/10.1007/BF00385097
2. Jia N., Zhu Y., Xie F. An efficient protocol for model legume root protoplast isolation and transformation. Frontiers in Plant Science. 2018;9:670. DOI: https://doi.org/10.3389/fpls.2018.00670
3. Cocking E. C. A method for the isolation of plant protoplasts and vacuoles. Nature. 1960;187:962-963. DOI: https://doi.org/10.1038/187962a0
4. Roest S., Gilissen L. J. W. Regeneration from protoplast a supplementary literature review. Acta Botanica Neerlandica. 1993;42(1):1-23. DOI: https://doi.org/10.1111/j.1438-8677.1993.tb00674.x
5. Laurila J., Laakso I., Larkka J., Gavrilenko T., Rokka V.-M., Pehu E. The proportions of glycoalkaloid aglycones are dependent on the genome constitutions of interspecific hybrids between two Solanum species (S. brevidens and S. tuberosum). Plant Science. 2001;161(4):677-683. DOI: https://doi.org/10.1016/s0168-9452(01)00453-8
6. Orczyk W., Przetakiewicz J., Nadolska-Orczyk A. Somatic hybrids of Solanum tuberosum – application to genetics and breeding. Plant Cell, Tissue and Organ Culture. 2003;74:1-13. DOI: https://doi.org/10.1023/A:1023396405655
7. Trabelsi S., Gargouri-Bouzid R., Vedel F., Nato A., Lakhoua L., Drira N. Somatic hybrids between potato Solanum tuberosum and wild species Solanum vernei exhibit a recombination in the plastome. Plant Cell, Tissue and Organ Culture. 2005;83:1-11. DOI: https://doi.org/10.1007/s11240-005-3667-3
8. Thieme R., Rakosy-Tican E., Gavrilenko T., Antonova O., Schubert J., Nachtigall M., Heimbach U., Thieme T. Novel somatic hybrids (Solanum tuberosum L. + Solanum tarnii) and their fertile BC1 progenies express extreme resistance to potato virus Y and late blight. Theoretical and Applied Genetics. 2008;116:691-700. DOI: https://doi.org/10.1007/s00122-007-0702-2
9. Gavrilenko T. A., Yermishin A. P. Interspecific hybridization of potato: theoretical and applied aspects. Vavilovskiĭ zhurnal genetiki i selektsii = Vavilov Journal of Genetics and Breeding. 2017;21(1):16-29. (In Russ.). DOI: https://doi.org/10.18699/VJ17.220
10. Perl A., Aviv D., Galun E. Protoplast-fusion-derived Solanum hybrids: application and phylogenetic limitations. Theoretical and Applied Genetics. 1990;79(5):632-640. DOI: https://doi.org/10.1007/BF00226876
11. Laferriere L., Helgeson J., Allen C. Fertile Solanum tuberosum+S. commersonii somatic hybrids as sources of resistance to bacterial wilt caused by Ralstonia solanacearum. Theoretical and Applied Genetics. 1999;98:1272-1278. DOI: https://doi.org/10.1007/s001220051193
12. Rokka V-M., Xu Y-S., Kankila J., Kuusela A., Pulli S, Pehu E. Identification of somatic hybrids of dihaploid Solanum tuberosum lines and S. brevidens by species specific RAPD patterns and assessment of disease resistance of the hybrids. Euphytica. 1994;80:207-217. DOI: https://doi.org/10.1007/BF00039652
13. Waara S., Glimelius К. The potential of somatic hybridization in crop breeding. Euphytica. 1995;85:217-233. DOI: https://doi.org/10.1007/BF00023951
14. Rokka V-M., Tauriainen A., Pietilä L., Pehu E. Interspecific somatic hybrids between wild potato Solanum acaule Bitt. and anther-derived dihaploid potato (Solanum tuberosum L.). Plant Cell Reports. 1998;18:82-88. DOI: https://doi.org/10.1007/s002990050536
15. Tiwari J. K., Devi S., Ali N., Luthra S. K., Kumar V., Bhardwaj V., Singh R. K., Rawat S., Chakrabarti S. K. Progress in somatic hybridization research in potato during the past 40 years. Plant Cell, Tissue and Organ Culture. 2018;132:225-238. DOI: https://doi.org/10.1007/s11240-017-1327-z
16. Iovene M., Savarese M., Cardi T., Frusciante L., Scott N., Simon P. W., Carputo D. Nuclear and cytoplasmic genome composition of Solanum bulbocastanum (+) S. tuberosum somatic hybrids. Genome. 2007;50(5):443-450. DOI: https://doi.org/10.1139/g07-024
17. Greplová M., Polzerová H., Vlastníková H. Electrofusion of pro- toplasts from Solanum tuberosum, S. bulbocastanum and S. pin- natisectum. Acta Physiologiae Plantarum. 2008;30:787. DOI: https://doi.org/10.1007/s11738-008-0183-1
18. Tiwari J. K., Poonam, Sarkar D., Pandey S. K., Gopal J., Kumar S. R. Molecular and morphological characterization of somatic hybrids between Solanum tuberosum L. and S. etuberosum Lindl. Plant Cell, Tissue and Organ Culture. 2010;103:175-187. DOI: https://doi.org/10.1007/s11240-010-9765-x
19. Sarkar D., Tiwari J. K., Sharma S. H., Poonam., Sharma S. A., Gopal J., Singh B. P., Luthra S. K., Pandey S. K., Pattanayak D. Production and characterization of somatic hybrids between Solanum tuberosum L. and S. pinnatisectum Dun. Plant Cell, Tissue and Organ Culture. 2011;107:427-440. DOI: https://doi.org/10.1007/s11240-011-9993-8
20. Rokka V-M., Laurila J., Tauriainen A., Laakso I., Larkka J., Metzler M., Pietiä L. Glycoalkaloid aglycone accumulations associated with infection by Clavibacter michiganensis ssp. sepedonicus in potato species Solanum acaule and Solanum tuberosum and their interspecific somatic hybrids. Plant Cell Reports. 2005;23:683-691. DOI: https://doi.org/10.1007/s00299-004-0868-x
21. Bidani A., Nouri-Ellouz O., Lakhoua L., Sihachakr D., Cheniclet C., Mahjoub A., Drira N., Gargouri-Bouzid R. Interspecific potato somatic hybrids between Solanum berthaultii and Solanum tuberosum L. showed recombinant plastome and improved tolerance to salinity. Plant Cell, Tissue and Organ Culture. 2007;91:179-189. DOI: https://doi.org/10.1007/s11240-007-9284-6
22. Nouri-Ellouz O., Triki M. A., Jbir-Koubaa R., Louhichi A., Charfeddine S., Drira N., Gargouri-Bouzid R. Somatic hybrids between potato and S. berthaultii show partial resistance to soil-borne fungi and potato virus Y. Journal of Phytopathology. 2016;164(7-8):485-496. DOI: https://doi.org/10.1111/JPH.12474
23. Ahn Y. K., Park T-H. Resistance to common scab developed by somatic hybrids between Solanum brevidens and Solanum tuberosum. Acta Agriculturae Scandinavica. 2013;63(7):595-603. DOI: https://doi.org/10.1080/09064710.2013.829867
24. Laurila J., Laasko I., Larkka J., Gavrilenko T., Rokka V-M., Pehu E. The proportions of glycoalkaloid aglycones are dependent on the genome constitutions of interspecific hybrids between two Solanum species (S. brevidens and S. tuberosum). Plant Science. 2001;161(4):677-683. DOI: https://doi.org/10.1016/S0168-9452(01)00453-8
25. Naess S. K., Bradeen J. M., Wielgus S. M., Haberlach G. T., McGrath J. M., Helgeson J. P. Resistance to late blight in Solanum bulbocastanum is mapped to chromosome 8. Theoretical and Applied Genetics. 2000;101:697-704. DOI: https://doi.org/10.1007/s001220051533
26. Rakosy-Tican E., Thieme R., Nachtigall M., Molnar I., Denes T-E. The recipient potato cultivar influences the genetic makeup of the somatic hybrids between five potato cultivars and one cloned accession of sexually incompatible species Solanum bulbocastanum Dun. Plant Cell, Tissue and Organ Culture. 2015;122:395-407. DOI: https://doi.org/10.1007/s11240-015-0777-4
27. Bołtowicz B., Szczerbakowa A., Wielgat B. RAPD analysis of the interspecific somatic hybrids Solanum bulbocastanum (+) S. tuberosum. Cellular & Molecular Biology Letters. 2005;10:51-162.
28. Szczerbakowa A., Tarwacka J., Oskiera M., Jakuczun H., Wielgat B. Somatic hybridization between the diploids of S. × michoacanum and S. tuberosum. Acta Physiologiae Plantarum. 2010;32:867-873. DOI: https://doi.org/10.1007/s11738-010-0472-3
29. Iovene M., Aversano R., Savarese S., Caruso I., Dimatteo A., Cardi T., Frusciante L., Carputo D. Interspecific somatic hybrids between Solanum bulbocastanum and S. tuberosum and their haploidization for potato breeding. Biologia Plantatum. 2012;56:1-8. DOI: https://doi.org/10.1007/s10535-012-0008-3
30. Sedlák P., Sedláková V., Vašek J., Zeka D., Čilová D., Melounová M., Orsák M., Domkářová J., Doležal P., Vejl P. Phenotypic, molecular and biochemical evaluation of somatic hybrids between Solanum tuberosum and S. bulbocastanum. Scientific Reports. 2022;12(1):4484. DOI: https://doi.org/10.1038/s41598-022-08424-5
31. Shi Y. Z., Chen Q., Li H. Y., Beasley D., Lynch D. R. Somatic hybridi- zation between Solanum tuberosum and S. cardiophyllum. Canadian Journal of Plant Science. 2006;86:539-545. DOI: https://doi.org/10.4141/P05-076
32. Thieme R., Rakosy-Tican E., Nachtigall M., Schubert J., Hammann T., Antonova O., Gavrilenko T., Heimbach U, Thieme T. Characterization of the multiple resistance traits of somatic hybrids between Solanum cardiophyllum Lindl. and two commercial potato cultivars. Plant Cell Reports. 2010;29:1187-1201. DOI: https://doi.org/10.1007/s00299-010-0905-x
33. Chandel P., Tiwari J. K., Ali N., Devi S., Sharma S. H., Sharma S. A., Luthra S. K., Singh B. P. Interspecific potato somatic hybrids between Solanum tuberosum and S. cardiophyllum, potential sources of late blight resistance breeding. Plant Cell, Tissue and Organ Culture. 2015;123:579-589. DOI: https://doi.org/10.1007/s11240-015-0862-8
34. Guo X., Xie C., Cai X., Song B., He L., Liu J. Meiotic behavior of pollen mother cells in relation to ploidy level of somatic hybrids between Solanum tuberosum and S. chacoense. Plant Cell Reports. 2010;29:1277-1285. DOI: https://doi.org/10.1007/s00299-010-0914-9
35. Chen L., Guo X., Xie C., He L., Cai X., Tian L., Song B., Liu J. Nuclear and cytoplasmic genome components of Solanum tuberosum + S. chacoense somatic hybrids and three SSR alleles related to bacterial wilt resistance. Theoretical and Applied Genetics. 2013;126:1861-1872. DOI: https://doi.org/10.1007/s00122-013-2098-5
36. Oberwalder B., Schilde-Rentschler L., Loffelhardt-Ruob B., Ninnemann H. Differences between hybrids of Solanum tuberosum L. and Solanum circaeifolium Bitt. obtained from symmetric and asymmetric fusion experiments. Potato Research. 2000;43:71-82. DOI: https://doi.org/10.1007/BF02358515
37. Kim-Lee H., Moon J. S., Hong Y. J., Kim M. S., Cho H. M. Bacterial wilt resistance in the progenies of the fusion hybrids between haploid of potato and Solanum commersonii. American Journal of Potato Research. 2005;82:129-137. DOI: https://doi.org/10.1007/BF02853650
38. Tiwari J. K., Devi S., Sundaresha S., Chandel P., Ali N., Singh B., Bhardwaj V., Singh B. P. Microarray analysis of gene expression pat- terns in the leaf during potato tuberization in the potato somatic hybrid Solanum tuberosum and Solanum etuberosum. Genome. 2015;58(6):305-313. DOI: https://doi.org/10.1139/gen-2014-0191
39. Gavrilenko T., Thieme R., Heimbach U., Thieme T. Fertile somatic hybrids of Solanum etuberosum + dihaploid Solanum tuberosum and their backcrossing progenies: relationships of genome dosage with tuber development and resistance to potato virus Y. Euphytica. 2003;131:323-332. DOI: https://doi.org/10.1023/A:1024041104170
40. Yu Y., Ye W., He L., Cai X., Liu T., Liu J. Introgression of bacterial wilt resistance from eggplant to potato via protoplast fusion and genome components of the hybrids. Plant Cell Reports. 2013;32:1687-1701. DOI: https://doi.org/10.1007/s00299-013-1480-8
41. Smyda P., Jakuczun H., Debski K., Śliwka J., Thieme R., Nachtigall M., Wasilewicz-Flis I., ZimnochGuzowska E. Development of somatic hybrids Solanum × michoacanum Bitter. (Rydb.) (+) S. tuberosum L. and autofused 4x S. × michoacanum plants as potential sources of late blight resistance for potato breeding. Plant Cell Reports. 2013;32:1231-1241. DOI: https://doi.org/10.1007/s00299-013-1422-5
42. Fock I., Collonier C., Purwito A., Luisetti J., Sonvannavong V., Vedel F., Servaes A., Ambroise A., Kodja H., Ducreux G., Sihachakr D. Resistance to bacterial wilt in somatic hybrids between Solanum tuberosum and S. phureja. Plant Science. 2000;160(1):165-176. DOI: https://doi.org/10.1016/s0168-9452(00)00375-7
43. Harding K., Millam S. Analysis of chromatin, nuclear DNA and organelle composition in somatic hybrids between Solanum tuberosum and Solanum sanctaerosae. Theoretical and Applied Genetics. 2000;101:939-947. DOI: https://doi.org/10.1007/s001220051565
44. Fock I., Collonier C., Purwito A., Luisetti J., Sonvannavong V., Vedel F., Servaes A., Ambroise A., Kodja H., Ducreux G., Sihachakr D. Use of S. stenotomum for introduction of resistance to bacterial wilt in somatic hybrids of potato. Plant Physiology and Biochemistry. 2001;39(10):899-908. DOI: https://doi.org/10.1016/S0981-9428(01)01307-9
45. Trabelsi S., Gargouri-Bouzid R., Vedel F., Nato A., Lakhoua L., Drira N. Somatic hybrids between potato Solanum tuberosum and wild species Solanum verneï exhibit a recombination in the plastome. Plant Cell, Tissue and Organ Culture. 2005;83:1-11. DOI: https://doi.org/10.1007/s11240-005-3667-3
46. Carrasco A., De Galarreta J. I. R., Rico A., Ritter E. Transfer of PLRV resistance from Solanum verrucosum Schlechdt to potato (S. tuberosum L.) by protoplast electrofusion. Potato Research. 2000;43:31-42. DOI: https://doi.org/10.1007/BF02358511
47. Tarwacka J., Polkowska-Kowalczyk L., Kolano B., Śliwka J., Wielgat B. Interspecific somatic hybrids Solanum villosum (+) S. tuberosum, resistant to Phytophthora infestans. Journal of Plant Physiology. 2013;170(17):1541-1548. DOI: https://doi.org/10.1016/j.jplph.2013.06.013
48. Murashige T., Skoog F. A Revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum. 1962;15(3):473-497. DOI: https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
49. Gamborg O. L., Miller R. A., Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research. 19628;50(1):151-158. DOI: https://doi.org/10.1016/0014-4827(68)90403-5
50. Kao K. N., Michayluk M. R. Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta. 1975;126:105-110. DOI: https://doi.org/10.1007/BF00380613
51. Binding H., Nehls R. Regeneration of isolated protoplasts to plants in Solanum dulcamara L. Zeitschrift für Pflanzenphysiologie. 1977;85(3):279-280. DOI: https://doi.org/10.1016/S0044-328X(77)80255-9
52. Binding H. Cell cluster formation by leaf protoplasts from axenic cultures of haploid Petunia hybrida L. Plant Science Letters. 1974;2(3):185-187. DOI: https://doi.org/10.1016/0304-4211(74)90018-2
53. Nagy J. I., Maliga P. Callus induction and plant regeneration from mesophyll protoplasts of Nicotiana sylvestris. Zeitschrift für Pflanzenphysiologie. 1976;78(5):453-455. DOI: https://doi.org/10.1016/s0044-328x(76)80093-1
54. Hunt G. J., Helgeson J. P. A medium and simplified procedure for growing single cells from Solanum species. Plant Science. 1989;60(2):251-257. DOI: https://doi.org/10.1016/0168-9452(89)90174-x
55. Senda M., Takeda J., Abe S., Nakamura T. Induction of cell fusion of plant protoplasts by electrical stimulation. Plant and Cell Physiology. 1979;20(7):1441-1443. DOI: https://doi.org/10.1093/OXFORDJOURNALS.PCP.A075944
56. Hammatt N., Lister A., Blackhall N. W., Gartland J., Ghose T. K., Gilmour D. M., Power J. B., Davey M. R., Cocking E. C. Selection of plant heterokaryons from diverse origins by flow cytometry. Protoplasma. 1990;154:34-44. DOI: https://doi.org/10.1007/BF01349533
57. Kulawiec M., Tagashira N., Plader W., Bartoszewski G., Kuć D., Sniezko R., Malepszy S. Chromosome number variation in somatic hybrids between transgenic tomato (Lycopersicon esculentum) and Solanum lycopersicoides. Journal of Applied Genetics. 2003;44(4):431-437.
58. Guri A., Sink K. C. Interspecific somatic hybrid plants between eggplant (Solanum melongena) and Solanum torvum. Theoretical and Applied Genetics. 1988;76:490-496. DOI: https://doi.org/10.1007/BF00260897
59. Sidorov V. A., Zubko M. K., Kuchko A. A., Komarnitsky I. K., Gleba Y. Y. Somatic hybridization in potato: use of gammairradiated protoplasts of Solanum pinnatisectum struction. Theoretical and Applied Genetics. 1987;74:364-368. DOI: https://doi.org/10.1007/BF00274719
60. Gleddie S., Keller W. A., Setteriield G. Production and characterization of somatic hybrids between Solanum melongena L. and Solanum sisymbriifolium Lam. Theoretical and Applied Genetics. 1986;71:613-621. DOI: https://doi.org/10.1007/BF00264265
61. Rakosy-Tican E., Aurori A. Green fluorescent protein (GFP) supports the selection based on callus vigorous growth in the somatic hybrids Solanum tuberosum L. + S. chacoense Bitt. Acta Physiologiae Plantarum. 2015;37:201. DOI: https://doi.org/10.1007/s11738-015-1946-0
62. Śliwka J., Jakuczun H., Chmielarz M., Hara-Skrzypiec A., Tomczyńska I., Kilian A., Zimnoch-Guzowska E. A resistance gene against potato late blight originating from Solanum × michoacanum maps to potato chromosome VII. Theoretical and Applied Genetics. 2012;124:397-406. DOI: https://doi.org/10.1007/s00122-011-1715-4
63. Śliwka J., Jakuczun H., Chmielarz M., Hara-Skrzypiec A., Tomczńska I., Kilian A, Zimnoch-Guzowska E. Late blight resistance gene from Solanum ruiz-ceballosii is located on potato chromosome X and linked to violet flower colour. BMC Genetics. 2012;13:11. DOI: https://doi.org/10.1186/1471-2156-13-11
64. Lössl A., Götz M., Braun A., Wenzel G. Molecular markers for cytoplasm in potato: male sterility and contribution of different plastid-mitochondrial configurations to starch production. Euphytica. 2000;116:221-230. DOI: https://doi.org/10.1023/A:1004039320227
65. Hosaka K., Sanetomo R. Development of rapid identification method for potato cytoplasm and its use for evaluating Japanese collections. Theoretical and Applied Genetics. 2012;125:1237-1251. DOI: https://doi.org/10.1007/s00122-012-1909-4
66. Kim S. R., Ahn Y. K., Kim T. G., Kang H. S., Song S. W., Kim B. C., Kang S. G. Breeding of a new cultivar ‘Jeseo’ with resistance to common scab. Korean Journal of Breeding Science. 2013;45(4):468-473. DOI: https://doi.org/10.9787/KJBS.2013.45.4.468
67. Novy R. G., Gillen A. M., Whitworth J. L. Characterization of the expression and inheritance of potato leafroll virus (PLRV) and potato virus Y (PVY) resistance in three generations of germplasm derived from Solanum etuberosum. Theoretical and Applied Genetics. 2007;114:1161-1172. DOI: https://doi.org/10.1007/s00122-007-0508-2
68. Cho K. S., Cheon K. S., Hong S. Y., Cho J. H., Im J. S., Mekapogu M., Yu Y. S., Park T. H. Complete chloroplast genome sequences of Solanum commersonii and its application to chloroplast genotype in somatic hybrids with Solanum tuberosum. Plant Cell Reports. 2016;35(10):2113-2123. DOI: https://doi.org/10.1007/s00299-016-2022-y
69. Smyda-Dajmund P., Sliwka J., Wasilewicz-Flis I., Jakuczun H., Zimnoch-Guzowska E. Genetic composition of interspecific potato somatic hybrids and autofused 4x plants evaluated by DArT and cytoplasmic DNA markers. Plant Cell Reports. 2016;35:1345-1358. DOI: https://doi.org/10.1007/s00299-016-1966-2
70. Singh R., Tiwari J. K., Rawat S., Sharma V., Singh B. P. Monitoring gene expression pattern in somatic hybrid of Solanum tuberosum and S. pinnatisectum for late blight resistance using microarray analysis. Plant Omics Journal. 2016;9(1):99-105. URL: https://www.pomics.com/tiwari_9_1_2016_99_105.pdf
71. Cho K.-S., Lee H.-O., Lee S.-C., Park H.-J., Cho J.-H., Park Y.-E., Choi J.-G., Yang T.-J. Mitochondrial genome recombination in somatic hybrids of Solanum commersonii and S. tuberosum. Scientific Reports. 2022;12:8659. DOI: https://doi.org/10.1038/s41598-022-12661-z
72. Sedlák P., Sedláková V., Vašek J., Zeka D., Čílová D., Melounová M., Orsák M., Domkářová J., Doležal P., Vejl P. Phenotypic, molecular and biochemical evaluation of somatic hybrids between Solanum tuberosum and S. bulbocastanum. Scientific Reports. 2022;12(1):4484. DOI: https://doi.org/10.1038/s41598-022-08424-5
73. Fossi M., Amundson K. R., Kuppu S., Britt A. B., Comai L. Regeneration of Solanum tuberosum plants from protoplasts induces widespread genome instability. Plant Physiology. 2019;180(1):78-86. DOI: https://doi.org/10.1104/pp.18.00906
74. Jiang M., Wu X., Song Y., Shen H., Cui H. Effects of OsMSH6 mutations on microsatellitest ability and homeologous recombination in rice. Frontiers in Plant Science. 2020;1:220. DOI: https://doi.org/10.3389/fpls.2020.00220
75. Dann A. L., Wilson C. R. Comparative assessment of genetic and epigenetic variation among regenerants of potato (Solanum tuberosum) derived from long-term nodal tissue-culture and cell selection. Plant Cell Reports. 2011;30(4):631-639. DOI: https://doi.org/10.1007/s00299-010-0983-9
Review
For citations:
Polivanova O.B., Egorova A.S., Sivolapova A.B., Goryunova S.V. Current state and prospects of protoplast technology and potato somatic hybridization (review). Agricultural Science Euro-North-East. 2023;24(1):7-19. (In Russ.) https://doi.org/10.30766/2072-9081.2023.24.1.7-19
Views:
1559
Email this article 