Bacterial community of agricultural soils used for potato cultivation in Sverdlovsk region
https://doi.org/10.30766/2072-9081.2023.24.6.989-998
Abstract
The yield of potatoes and other crops is influenced by many factors, one of the most important is the complex condition of the soil. Soil research more often focuses on the determination of its physical and chemical properties, but rarely takes into account the bacterial community and its diversity. In this work, the bacterial microbiota of soils cultivated with potato was evaluated. Using metabarcoding and full-fragment sequencing of the 16S rRNA site, by nanopore sequencing, primary screening of the bacterial community of fields in three administrative districts of the Sverdlovsk region: the city of Yekaterinburg, Beloyarsky and Sysertsky districts was carried out in 2022. As a result, 2371 operational taxonomic units (OTUs) were identified to the species level. More than half of the relative bacterial abundance is occupied by the phylum Proteobacteria. Three orders represent more than one-third of the total bacterial community: Burkholderiales, Hyphomicrobiales, and Acidobacteriales. The most common bacterial genera in cultivated agricultural soils of the Sverdlovsk region are Bradyrhizobium, Massilia, Gaiella, Sphingomonas, Lysobacter and Gemmatimonas. The obtained results of alpha- and beta-diversity analysis allow us to conclude that, despite the statistically significant difference in the number of detected OTUs between some fields, there is no difference in their diversity by study objects in the administrative districts of the Sverdlovsk region.
Supporting agencies: The work was carried out with the support of the Ministry of Science and Higher Education of the Russian Federation as part of the implementation of the CSRP subprogram "Breeding and seed production of potatoes". The authors thank the reviewers for their contribution to the expert evaluation of this work.
About the Authors
E. P. Shanina
Ural Federal Agrarian Scientific Research Centre, Ural Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
Elena P. Shanina, DSc in Agricultural Science, chief researcher
21, Glavnaya St., Yekaterinburg, 620061
G. A. Lihodeevskiy
Ural Federal Agrarian Scientific Research Centre, Ural Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
Georgiy A. Lihodeevskiy, junior researcher
21, Glavnaya St., Yekaterinburg, 620061
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For citations:
Shanina E.P., Lihodeevskiy G.A. Bacterial community of agricultural soils used for potato cultivation in Sverdlovsk region. Agricultural Science Euro-North-East. 2023;24(6):989-998. (In Russ.) https://doi.org/10.30766/2072-9081.2023.24.6.989-998
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