Screening of the strawberry collection for the presence of resistance loci Rca2 and 08 To-f
https://doi.org/10.30766/2072-9081.2025.26.3.546-554
Abstract
Garden strawberry (Fragaria × ananassa Duch.) is one of the most common cultivated berry crops. Fungal diseases cause enormous damage to industrial plantings. The main task of modern breeding is to create strawberry cultivars with genetic determinants of resistance to various pathogens. The aim of the research was to identify the resistance loci of Russian and foreign strawberry cultivars to anthracnose (Rca2) and powdery mildew (08 To-f) using DNA markers. The objects of the study were 32 strawberry cultivars from the Udmurt Federal Research Center of the Ural Branch of the Russian Academy of Sciences collection, including 21 samples of Russian strawberry cultivars and 11 of foreign breeding. The dominant diagnostic DNA markers STS-Rca2_240 and IB535110 were used to identify the resistance genes of the strawberry. PCR monitoring was performed using the EMFv020 marker. As a result of molecular genetic screening, the anthracnose resistance gene Rca2 was detected in one Russian strawberry cultivar (‘Krasnaya pchelka’, Russia) and in one foreign cultivar (‘Malling Pandora’, Great Britain). QTL 08 To-f was detected in four cultivars of the collection: one Russian strawberry cultivar (‘Duet’) and three foreign cultivars (‘Malwina’, Germany; ‘Marmion’, Scotland; ‘Malling Pandora’). In total, among the analyzed strawberry genotypes, marker STS-Rca2_240 was detected in 6.3 % of the studied forms, and the marker IB535110 in 12.5 %. The complex source of Rca2 and QTL 08 To-f resistance alleles was only the ‘Malling Pandora’ cultivar. The identified promising genetic sources will be used in the breeding program for producing of fungal pathogens resistant garden strawberry cultivars.
Keywords
Fragaria × ananassa,
anthracnose,
powdery mildew,
resistance,
DNA markers,
polymerase chain reaction
Supporting agencies: the research was carried out under the support of the Ministry of Science of the Russian Federation within the state assignment of the Udmurt Federal Research Center of the Ural Branch of the Russian Academy of Sciences (theme No. FUUE-2024-0012).
About the Authors
A. V. Khudyakova
Udmurt Federal Research Center of the Ural Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
Anna V. Khudyakova, researcher
st. T. Baramzinoj, 34, Izhevsk, Udmurt Republic, 426067
M. G. Markova
Udmurt Federal Research Center of the Ural Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
Marina G. Markova, researcher
st. T. Baramzinoj, 34, Izhevsk, Udmurt Republic, 426067
References
1. Lyzhin A. S., Luk’yanchuk I. V. Genetic diversity in wild species and cultivars of strawberry for the FanAAMT gene controlling fruit flavor volatiles. Trudy po prikladnoy botanike, genetike i selektsii = Proceedings on applied botany, genetics and breeding. 2021;182(2):72–80. (In Russ.). DOI: https://doi.org/10.30901/2227-8834-2021-2-72-80
2. Whitaker V. M., Knapp S. J., Hardigan M. A., Edger P. P., Slovin J. P., Bassil N. V. et al. A roadmap for research in octoploid strawberry. Horticulture Research. 2020;7:33. DOI: https://doi.org/10.1038/s41438-020-0252-1
3. Marchenko L. A. Research methods and ways in strawberry breeding problems solution (Analytical review). Vestnik KrasGAU = The Bulletin of KrasGAU. 2021;(9):59–68. (In Russ.). DOI: https://doi.org/10.36718/1819-4036-2021-9-59-68
4. Nevostrueva E. Yu., Andreeva G. V., Pavlova O. A. Improving the assortment of strawberries for the VolgaVyatka region. Sovremennoe sadovodstvo = Cotemporary horticulture. 2021;(1):28–35. (In Russ.). DOI: https://doi.org/10.52415/23126701_2021_0104
5. Kulikov I. M., Evdokimenko S. N., Tumaeva T. A., Kelina A. V., Sazonov F. F., Andronova N. V., Podgaetskiy M. A. Scientific support of small fruit growing in Russia and prospects for its development. Vavilovskiy zhurnal genetiki i selektsii = Vavilov Journal of Genetics and Breeding. 2021;25(4):414–419. (In Russ.). DOI: https://doi.org/10.18699/VJ21.046
6. Deyneko E. V. Modification of the plant genome by genetic engineering methods: directions and ways of development. Fiziologiya rasteniy = Russian Journal of Plant Physiology. 2024;71(5):487–501. (In Russ.). DOI: https://doi.org/10.31857/s0015330324050017
7. Chekushkina T. N., Barsukova E. N. Biotechnological methods in the selection of Fragaria × Ananassa Duch. (review). Agrarnaya Rossiya = Agrarian Russia. 2022;(12):12–18. (In Russ.). DOI: https://doi.org/10.30906/1999-5636-2022-12-12-18
8. Rakhmangulov R. S., Barabanov I. V., Erastenkova M. V., Ivanov A. A., Kovalenko T. V., Mezhina K. M. et al. The new directions in genetics, breeding and biotechnology of ornamental and berry crops in the N.I. Vavilov Institute of Plant Genetic Resources (VIR). Biotekhnologiya i selektsiya rasteniy = Plant Biotechnology and Breeding. 2022;5(4):65–78. (In Russ.). DOI: https://doi.org/10.30901/2658-6266-2022-4-o3
9. Salinas N., Fan Z., Peres N., Lee S., Whitaker V. M. FaRCa1 confers moderate resistance to the root necrosis form of strawberry anthracnose caused by Colletotrichum acutatum. HortScience. 2020;55(5):693–698. DOI: https://doi.org/10.21273/HORTSCI14807-20
10. Tsvetkova Yu. V., Kuznetsova A. A. Identification of strawberry anthracnose and methods for diagnosing causative agents of the disease. Mikologiya i fitopatologiya = Mycology and Phytopathology. 2022;56(2):140–152. (In Russ.). DOI: https://doi.org/10.31857/S0026364822020118
11. Khrabrov I. E., Antonova O. Yu., Shapovalov M. I., Semenova L. G. Molecular screening of the VIR strawberry varieties collection for the presence of a marker for the anthracnose black rot resistance gene Rca2. Biotekhnologiya i selektsiya rasteniy = Plant Biotechnology and Breeding. 2021;4(4):15–24. (In Russ.). DOI: https://doi.org/10.30901/2658-6266-2021-4-o3
12. Lyzhin A. S., Lukyanchuk I. V. Allelic diversity of pathogen resistance loci in russian strawberry varieties and prospects for improving the assortment. Tavricheskiy vestnik agrarnoy nauki = Taurida herald of the agrarian sciences. 2024;(4(40)):147–157. (In Russ.). DOI: https://doi.org/10.5281/zenodo.14184676
13. Khrabrov I. E., Antonova O. Yu., Shapovalov M. I., Semenova L. G. Strawberry resistance to the major fungal phytopathogens: R-genes and their DNA markers. Biotekhnologiya i selektsiya rasteniy = Plant Biotechnology and Breeding. 2019;2(3):30–40. (In Russ.). DOI: https://doi.org/10.30901/2658-6266-2019-3-o3
14. Lyzhin A. S., Lukyanchuk I. V. Molecular screening of strawberry accessions from the “I.V. Michurin FSC” genetic collection by 08 To-f powdery mildew resistance locus. Tavricheskiy vestnik agrarnoy nauki = Taurida herald of the agrarian sciences. 2024;(1(37)):103–111. (In Russ.). DOI: https://doi.org/10.5281/zenodo.10926231
15. Lyzhin A. S., Lukyanchuk I. V. Molecular screening of resistance alleles 08 To-f, Rca2 and Rpf1 in strawberry hybrid progeny for identify forms with complex resistance to fungal pathogens. Pis'ma v Vavilovskiy zhurnal genetiki i selektsii = Letters to the Vavilov Journal of Genetics and Breeding. 2024;10(2):105–110. (In Russ.). DOI: https://doi.org/10.18699/letvjgb-2024-10-12
16. Koishihara H., Enoki H., Muramatsu M., Nishimura S., Susumu Y. U. I., Honjo M. Marker associated with powdery mildew resistance in plant of genus Fragaria and use there of U.S.: Patent No. 10,724,093. Washington, DC: U.S. Patent and Trademark Office. 2020.
17. Hadonou A. M., Sargent D. J., Wilson F., James C. M., Simpson D. W. Development of microsatellite markers in Fragaria, their use in genetic diversity analysis, and their potential for genetic linkage mapping. Genome. 2004;47(3):429–438. DOI: https://doi.org/10.1139/g03-142
18. Lerceteau-Kohler E., Guerin G., Denoyes-Rothan B. Identification of SCAR markers linked to Rca2 anthracnose resistance gene and their assessment in strawberry germplasm. Theoretical and Applied Genetics. 2005;111:862–870. DOI: https://doi.org/10.1007/s00122-005-0008-1
19. Lyzhin A. S., Lukyanchuk I. V. Еhe use of DNA markers in strawberry breeding for pathogen resistance. Sovremennoe sadovodstvo = Cotemporary horticulture. 2023;(4):12–22. (In Russ.). DOI: https://doi.org/10.52415/23126701_2023_0402
20. Lyzhin A. S., Lukyanchuk I. V. Study of a genetic collection of strawberry (Fragaria L.) for resistance to powdery mildew. Vavilovskiy zhurnal genetiki i selektsii = Vavilov Journal of Genetics and Breedin. 2024;28(2):166–174. (In Russ.). DOI: https://doi.org/10.18699/vjgb-24-19
21. Nevostrueva E. Yu., Bagmet L. V. Nomenclatural standards of strawberry cultivars bred by Sverdlovsk Horticultural Breeding Station. Agrarnaya nauka Evro-Severo-Vostoka = Agricultural Science Euro-North-East. 2024;25(5):846–854. (In Russ.). DOI: https://doi.org/10.30766/2072-9081.2024.25.5.846-854
22. Keldibekova M., Bezlepkina E., Zubkova M., Dolzhikova M. DNA-screening of strawberry cultivars and hybrids (Fragaria ananassa Duch.) for resistance to fungal diseases. Pakistan Journal of Botany. 2024;56(2):29. DOI: https://doi.org/10.30848/PJB2024-2(29)
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For citations:
Khudyakova A.V., Markova M.G. Screening of the strawberry collection for the presence of resistance loci Rca2 and 08 To-f. Agricultural Science Euro-North-East. 2025;26(3):546-554. (In Russ.) https://doi.org/10.30766/2072-9081.2025.26.3.546-554
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