Evaluation of original strawberry forms for resistance to gray mold in the conditions of the Middle Urals
https://doi.org/10.30766/2072-9081.2025.26.1.90-97
Abstract
Gray mold is one of the main harmful diseases of strawberries. Breeding for resistance to this disease still begins with identifying resistant phenotypes and using them in crosses. Since strawberries are a polygenic, heterozygous species, then in order to increase the effectiveness of breeding work, it is necessary to conduct a breeding assessment of the original forms for resistance to gray mold. The studies involved cultivars and forms of breeding of the Sverdlovsk breeding station of horticulture – ‘Duet’, ‘Forsazh’, ‘Gejzer’ 1-14-10, 2-43-10, 3-44-10, 2-45-10, 3-45-10, 2-54-11, introduced cultivars ‘Ol'viya’, ‘Solovushka’, ‘Belrubi’, ‘Cardinal’ and their hybrid offspring – 600 seedlings of 10 families. The maximum development of gray mold on the original forms of strawberries was observed in the years of epiphytoties – 2017, 2018, 2024. According to the results of the surveys, only two forms 2-45-10 and 2-54-11 were resistant to this disease. The hybrid offspring was assessed in 2024 against a natural phytopathogenic background. According to the results of breeding assessment for the yield of disease-resistant seedlings (29.0–34.1 %), families were identified in the origin of both parental forms of which cultivars with dense berries participated – ‘Marmolada’ and ‘Totem’: 1-40-10 × 3-44-10; 2-43-10 × 2-54-11; ‘Forsazh’ × 2-43-10 (29.0–34.1 %). The use of resistant forms 2-45-10 and 2-54-11 in crossings (except for the family 2-43-10 × 2-54-11) gave a lower yield of seedlings without signs of the disease (6.3–16.7 %). During the research, 10 selected strawberry seedlings were selected, combining in their genotype, in addition to resistance to gray mold, a set of economically valuable traits. These seedlings represent new source material for further selection work.
Keywords
About the Author
Elena Yu. NevostruevaRussian Federation
Elena Yu. Nevostrueva, PhD in Agricultural Science, senior researcher, Ural Federal Agrarian Scientific Research Centre
112, bldg. A, Belinskogo Street, Yekaterinburg, 620142
References
1. Shamanskaya L. D. Pests and diseases of Siberian gardens. Barnaul: Novyy format, 2018. pp. 133–134.
2. Petrasch S., Knapp S. J., van Kan J. A. L., Blanco-Ulate B. Grey mould of strawberry, a devastating disease caused by the ubiquitous necrotrophic fungal pathogen Botrytis cinerea. Molecular Plant Pathology. 2019;20(6):877–892. DOI: https://doi.org/10.1111/mpp.12794
3. Guo J., Wang S., XiaoYang Y., Dong R., Li Y., Mei X., Shen Y. Polyamines regulate strawberry fruit ripening by ABA, IAA, and ethylene. Plant Physiology. 2018;177(1):339–351. DOI: https://doi.org/10.1104/pp.18.00245
4. Sánchez-Sevilla J. F., Vallarino J. G., Osorio S., Bombarely A., Posé D., Merchante C., et al. Gene expression atlas of fruit ripening and transcriptome assembly from RNA-Seq data in octoploid strawberry (Fragaria × ananassa). Scientific Reports. 2017;7:13737. DOI: https://doi.org/10.1038/s41598-017-14239-6
5. Xiao G., Zhang Q., Zeng X., Chen X., Liu S., Han Y. Deciphering the Molecular Signatures Associated With Resistance to Botrytis cinerea in Strawberry Flower by Comparative and Dynamic Transcriptome Analysis. Frontiers in Plant Science. 2022;13:888939. DOI: https://doi.org/10.3389/fpls.2022.888939
6. Leroch M., Plesken C., Weber R. W. S., Kauff F., Scalliet G., Hahn M. Gray mold populations in German strawberry fields are resistant to multiple fungicides and dominated by a novel clade closely related to Botrytis cinerea. Applied and Environmental Microbiology. 2013;79(1):159–167. DOI: https://doi.org/10.1128/AEM.02655-12
7. Vanti G. L., Leshem Y., Masaphy S. Resistance response enhancement and reduction of Botrytis cinerea infection in strawberry fruit by Morchella conica mycelial extract. Postharvest Biology and Technology. 2021;175:111470. DOI: https://doi.org/10.1016/j.postharvbio.2021.111470
8. Maksimov I. V., Singkh B. P., Cherepanova E. A., Burkhanova G. F., Khayrullin R. M. Prospects and applications of lipopeptide-producing bacteria for plant protection (review). Prikladnaya biokhimiya i mikrobiologiya. 2020;56(1):19–34. (In Russ.). DOI: https://doi.org/10.31857/S0555109920010134
9. Kholod N. A., Maslienko L. V. Biological control of strawberry gray rot under the conditions of increase in abiotic and anthropogenic factors. Nauchnye trudy Severo-Kavkazskogo federal'nogo nauchnogo tsentra sadovodstva, vinogradarstva, vinodeliya. 2018;14:179–183. (In Russ.). DOI: https://doi.org/10.30679/2587-9847-2018-14-179-183
10. Petrasch S., Mesquida-Pesci S. D., Pincot D. D. A., Feldmann M. J., López C. M., Famula R.,et al. Genomic prediction of strawberry resistance to postharvest fruit decay caused by the fungal pathogen Botrytis cinerea. G3: Genes. Genomes. Genetics. 2022;12(1):jkab378. DOI: https://doi.org/10.1093/g3journal/jkab378
11. Khrabrov I. E., Antonova O. Yu., Shapovalov M. I., Semenova L. G. Strawberry resistance to the major fungal phytopathogens: R-genes and their DNA markers. Biotekhnologiya i selektsiya rasteniy = Plant Biotechnology and Breeding. 2019;2(3):30-40. (In Russ.). DOI: https://doi.org/10.30901/2658-6266-2019-3-03
12. Lyzhin A. S., Luk’yanchuk I. V. Molecular screening of resistance alleles 08 TO-F, RCA2 and RPF1 in strawberry hybrid progeny for identify forms with complex resistance to fungal pathogens. Pis'ma v Vavilovskiy zhurnal genetiki i selektsii = Letters to the Vavilov Journal of Genetics and Breeding. 2024;10(2):105–110. (In Russ.). DOI: https://doi.org/10.18699/letvjgb-2024-10-12
13. Hébert C., Charles M. T., Gauthier L., Willemot C., Khanizadeh S., Cousineau J. Strawberry Proanthocyanidins: biochemical markers for Botritis cinerea resistance and shelf-life predictability. Acta Horticulturae. 2002;567:143. DOI: https://doi.org/10.17660/ActaHortic.2002.567.143
14. Zubov A. A. Theoretical foundations of strawberry breeding. Michurinsk, 2004. pp. 45–121.
15. Kichina V. V. Genetics and breeding of berry crops. Moscow: Kolos, 1984. pp. 223–250.
Review
For citations:
Nevostrueva E.Yu. Evaluation of original strawberry forms for resistance to gray mold in the conditions of the Middle Urals. Agricultural Science Euro-North-East. 2025;26(1):90-97. (In Russ.) https://doi.org/10.30766/2072-9081.2025.26.1.90-97