Dynamics of the development of live weight in cows of the Kholmogory breed according to the polymorphism of the leptin gene

To improve the efficiency of dairy production, it is necessary to pay attention to the quality of rearing young cattle the growth and development of which depend not only on paratypical, but also on genetic factors. The research goal of the work was to identify the relationship between the leptin gene type (site A80V) and changes in live weight indicators in cows of the Kholmogory breed at different age periods. The studied livestock included 330 cows of the Kholmogory breed from the Arkhangelsk region. The whole blood was used as biomaterial. The results were visualized by electrophoresis in 2 % agarose gel with a molecular weight marker Step50 (Biolabmix LLC, Russia). The results of the study showed the predominance of carriers of the A allele, therefore, the AA genotype was the most common. Animals with the AV genotype had the highest weight gain over 6, 10, 12 and 18 months. The age of the first insemination, the first calving and the first fruitful insemination did not differ significantly. However, significant differences in live weight were found at the first insemination between groups with AV and VV genotypes (+16.94 kg), as well as between animals with AA and VV genotypes (+15.49 kg). The average weight gain per month was also higher in animals with the AV genotype (+18.03±0.31 kg). It has been established that the most preferred genotype of the leptin gene of the A80V site in the sample of cows of the Kholmogorsky breed is the AV genotype. The further study of the leptin gene and its effects will help to gain a more complete understanding of its role in shaping the growth of living tissue in animals.

1. Chasovshchikova M. A., Kozlov K. A. Productive longevity of the black-and-white breed of cows in relation to age and live weight at the first insemination. Agroprodovol'stvennaya politika Rossii = Agri-Food Policy in Russia. 2021;(4):37–40. (In Russ.). URL: https://www.elibrary.ru/item.asp?id=47861957

2. Wangchuk K., Wangdi J., Mindu M. Comparison and reliability of techniques to estimate live cattle body weight. Journal of Applied Animal Research. 2017;46(1):349–352 DOI: https://doi.org/10.1080/09712119.2017.1302876

3. Kovalyuk N. V., Gyrnets E. A. Polymorphism of lep gene allele as a genetic marker of functional longevity of ayrshire cattle. Universum: khimiya i biologiya. 2016;(6(24)):3. (In Russ.). URL: http://7universum.com/ru/nature/archive/item/3257

4. Komendant T. M., Epishko O. A., Cheburanova E. S. Optimization of the method of detecting polymorphisms of the leptin gene (LEP), affecting the productive longevity of cattle. Agriculture – problems and prospects: collection of scientific papers. Grodno: Grodnenskiy GAU, 2017. Vol. 37. pp. 99–105. URL: https://www.elibrary.ru/item.asp?edn=yozazo

5. Lemyakin A. D., Tyazhchenko A. N., Sabetova K. D., Chaitskiy A. A., Shchegolev P. O., Korolev A. A. Reproductive ability of cows of domestic dairy breeds with different allelic variants of the leptin gene. Agrarnaya nauka Evro-Severo-Vostoka = Agricultural Science Euro-North-East. 2022;23(6):884–895. (In Russ.). DOI: https://doi.org/10.30766/2072-9081.2022.23.6.884-895

6. Zaytseva O. S., Bytov M. V., Volskaya S. V., Martynov N. A., Osipova Yu. A., Yusupova Ch. R. Assessment of allelic diversity of the leptin gene and associations of its single nucleotide polymorphismswith biochemical markers of predisposition to ketosis in cows. Problemy biologii produktivnykh zhivotnykh = Problems of Productive Animal Biology. 2024;(2):45–55. (In Russ.). DOI: https://doi.org/10.25687/1996-6733.prodanimbiol.2024.2.45-55

7. Kovalyuk N. V., Satsuk V. F., Machulskaya E. V., Shakhnazarova Yu. Yu. Possible causes and consequences of the distribution of separate allelic variants of the LEP gene in the groups of ayrshire and holstein cattle. Genetika = Russian Journal of Genetics. 2018;54(12):1442–1447. (In Russ.). DOI: https://doi.org/10.1134/S0016675818120068

8. Shchegolev P. O., Sabetova K. D., Chaitskiy A. A., Sorokina A. Relationship of leptin gene polymorphism (LEP) with economically useful traits of cattle. Agrarnyy vestnik Nechernozem'ya = Agrarian Bulletin of the NonChernozem region. 2021;(1(1)):25–32. (In Russ.). DOI: https://doi.org/10.52025/2712-8679_2021_01_25

9. Gaynutdinova E. R., Safina N. Yu., Shakirov Sh. K., Varlamova M. I. Influence of leptin (LEP) gene polymorphism on dairy and meat productivity of holstein heifers. Uchenye zapiski Kazanskoy gosudarstvennoy akademii veterinarnoy meditsiny im. N. E. Baumana = Scientific Notes Kazan Bauman State Academy of Veterinary Medicine. 2021;245(1):24–28. (In Russ.). DOI: https://doi.org/10.31588/2413-4201-1883-245-1-24-28

10. Kovalyuk N. V., Satsuk V. F., Machulskaya E. V., Morkovkina N. A., Shakhnazarova Yu. Yu. Use of polymorphism at loci of LEP in breeding of black-motley cattle. Molochnoe i myasnoe skotovodstvo = Journal of Dairy and Beef Cattle Farming. 2017;(3):14–16. (In Russ.). URL: https://www.elibrary.ru/item.asp?id=29389861

11. Kosyan D. B., Rusakova E. A. Relationship of lep/a80v gene polymorphism with growth characteristics of cattle. Byulleten' Orenburgskogo nauchnogo tsentra UrO RAN. 2019;(4):26. (In Russ.). URL: https://www.elibrary.ru/item.asp?id=42503559

12. Dubovskova M. P., Gerasimov N. P. Genetic structure and association of growth hormone (L127V) and leptin (A80V) gene polymorphism with productivity in the north caucasian population of the hereford breed. Zhivotnovodstvo i kormoproizvodstvo = Animal Husbandry and Fodder Production. 2020;103(3):91–101. (In Russ.). DOI: https://doi.org/10.33284/2658-3135-103-3-91

13. Kovalyuk N. V., Satsuk V. F., Volchenko A. E., Machulskaya E. V. LEP gene allelic polymorphism in a subpopulation of ayrshire cattle. Genetika = Russian Journal of Genetics. 2015;51(2):266–270. (In Russ.). DOI https://doi.org/10.7868/S0016675815020101

14. Mayorov V. A., Kozlovskaya A. Yu., Leontev A. A., Afanasev K. A. The duration of economic use of cows due to the age of the first calving. Izvestiya Velikolukskoy gosudarstvennoy sel'skokhozyaystvennoy akademii. 2015;(4):8–11. (In Russ.). URL: https://www.elibrary.ru/vmhdbf

15. Novotolskaya O. P., Kozlovskaya A. Yu., Leontev A. A., Skoptsova T. I., Kozlovskiy V. Yu. Reproductive ability indicators of ayrshire cows of various origin. Agrarnyy vestnik Urala = Agrarian Bulletin of the Urals. 2014;(1(119)):47–50. (In Russ.). URL: https://www.elibrary.ru/item.asp?id=21681381

16. Petrukhina L. L., Belozertseva S. L. Influence of the age at the first insemination and live weight on milk productivity of cows. Sibirskiy vestnik sel'skokhozyaystvennoy nauki = Siberian Herald of Agricultural Science. 2020;50(2):57–63. (In Russ.). DOI: https://doi.org/10.26898/0370-8799-2020-2-7

17. Mamatova N. D. The influence of the first insemination age on productive life-span of cows. Vestnik Altayskogo gosudarstvennogo agrarnogo universiteta = Bulletin of Altai State Agricultural University. 2018;(3(161)):110–114. (In Russ.). URL: http://vestnik.asau.ru/index.php/vestnik/article/view/1389.