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Optimization of methods for assessing the resistance of winter rye to snow mold in natural and artificial infection backgrounds

https://doi.org/10.30766/2072-9081.2025.26.3.564-576

Abstract

Snow mold is an economically important and progressive disease of winter rye caused by several species of low temperature fungi (ascomycetes and basidiomycetes) and fungal-like organisms (oomycetes), with yield losses of up to 30–50 %. The aim of this work is to improve methods for field evaluation of winter rye resistance to snow mold, both under artificial infestation and natural conditions. The research was carried out in 2019–2024 in the Republic of Tatarstan. Since 2021, the most aggressive strains of pink (Microdochium nivale), grey (Typhula incarnata) and speckled (T. ishikariensis) snow mold pathogens collected in the Volga Federal District have been used for artificial infection. The experiment involved 60 winter rye samples, including cultivars bred by Tatar Research Institute of Agriculture, FRC Kazan Scientific Center of RAS. For a more accurate assessment of the degree of damage and identification of resistant genotypes, a detailed 9-point scale was developed, with 1 − no damage and 9 − complete damage. Under natural conditions, the average damage point ranged from 2.41 to 5.15, while under artificial infestation it was significantly higher, from 4.48 to 8.40. Most winter rye cultivars showed a moderate to moderately susceptible type of reaction on a natural background in four out of 5 years of observation. The frequency of susceptible samples on the artificial infection background reached 96 % in 2024. The use of artificial infection backgrounds allows snow mold damage to be assessed independently of weather conditions and speeds up the breeding process, providing an objective assessment of genotype resistance. As a result of research, the methodology for establishing autonomous infection backgrounds has been optimized. It involves the selection of a site with a long snowmelt period and flat relief, the use of inoculum containing virulent strains of pathogens characteristic of the region and a special method of introducing the pathogen.

About the Authors

M. L. Ponomareva
Tatar Scientific Research Institute of Agriculture − Subdivision of the Federal State Budgetary Institution of Science "Kazan Scientific Center of the Russian Academy of Sciences"
Russian Federation

Mira L. Ponomareva, DSc in Biological Science, professor, chief researcher 

Orenburg tract, 48, Kazan, Tatarstan Republic, 420059



S. N. Ponomarev
Tatar Scientific Research Institute of Agriculture − Subdivision of the Federal State Budgetary Institution of Science "Kazan Scientific Center of the Russian Academy of Sciences"
Russian Federation

Sergey N. Ponomarev, DSc in Agricultural Science, chief researcher 

Orenburg tract, 48, Kazan, Tatarstan Republic, 420059



G. S. Mannapova
Tatar Scientific Research Institute of Agriculture − Subdivision of the Federal State Budgetary Institution of Science "Kazan Scientific Center of the Russian Academy of Sciences"
Russian Federation

Gulnaz S. Mannapova, PhD in Agricultural Science, leading researcher 

Orenburg tract, 48, Kazan, Tatarstan Republic, 420059



S. Yu. Pavlova
Tatar Scientific Research Institute of Agriculture − Subdivision of the Federal State Budgetary Institution of Science "Kazan Scientific Center of the Russian Academy of Sciences"
Russian Federation

Svetlana Yu. Pavlova, postgraduate student, junior researcher 

Orenburg tract, 48, Kazan, Tatarstan Republic, 420059



I. O. Ivanova
Tatar Scientific Research Institute of Agriculture − Subdivision of the Federal State Budgetary Institution of Science "Kazan Scientific Center of the Russian Academy of Sciences"
Russian Federation

Irina O. Ivanova, postgraduate student, junior researcher 

Orenburg tract, 48, Kazan, Tatarstan Republic, 420059



References

1. Ponomareva M. L., Gorskov V. Y., Ponomarev S. N., Korzun V., Miedaner Th. Snow mold of winter cereals: A complex disease and a challenge for resistance breeding. Theoretical and Applied Genetics. 2021;134(2):419–433. DOI: https://doi.org/10.1007/s00122-020-03725-7

2. Hoshino T., Xiao N., Tkachenko O. B. Cold adaptation in the phytopathogenic fungi causing snow molds. Mycoscience. 2009;50(1):26–38. DOI: https://doi.org/10.1007/S10267-008-0452-2

3. Beloshapkina O. O., Savos'kina O. A., Chebanenko S. I., Tarakanov R. I., Dzhalilov F. S.-U. Development of snow mold of winter wheat in the conditions of the central Non-chernozem region with regard to tillage technologies and weather conditions of different years. Izvestiya Timiryazevskoy sel'skokhozyaystvennoy akademii = Izvestiya of Timiryazev Agricultural Academy. 2023;(4):47–57. (In Russ.). DOI: https://doi.org/10.26897/0021-342X-2023-4-47-57

4. Smith J. D., Jackson N., Woolhouse A. R. Fungal Diseases of Amenity Turfgrass. London: E&F.N. Spon Ltd., 1989. 401 p.

5. Sakhabutdinov I. T., Chastukhina I. B., Ryazanov E. A., Ponomarev S. N., Gogoleva O. A., Balkin A. S., et al. Variability of microbiomes in winter rye, wheat, and triticale affected by snow mold: predicting promising microorganisms for the disease control. Environmental Microbiome. 2025;20(1):3. DOI: https://doi.org/10.1186/s40793-025-00665-x

6. Tkachenko O. B. Snow molds: history of study, pathogens, their biological features. Moscow: RAS, 2017. 71 p.

7. Hockemeyer K., Gonzalez Vazquez A., Koch P. L. Persistence of fungicides applied to control snow mold of turfgrass as influenced by snow cover and antitranspirants. Plant Disease. 2024;108(7):2034–2045. DOI: https://doi.org/10.1094/PDIS-05-23-0948-RE

8. Gagkaeva T. Y., Orina A. S., Gavrilova O. P., Gorina N. N. Evidence of Microdochium fungi associated with cereal grains in Russia. Microorganisms. 2020;8(3):340. DOI: https://doi.org/10.3390/microorganisms8030340

9. Gannibal F. B., Gagkaeva T. Yu., Gomzhina M. M., Poluektova E. V., Gultyaeva E. I. Micromycetes associated with wheat and their significance as pathogens in Russia. Vestnik zashchity rasteniy = Plant Protection News. 2022;105(4):164–180. (In Russ.). DOI: https://doi.org/10.31993/2308-6459-2023-106-4-16116

10. Tredway L. P., Tomaso-Peterson M., Kerns J. P., Clarke B. B. Compendium of turfgrass diseases. USA: APS Press, 2023. 4th ed.

11. Urban E. P., Gordey S. I., Artyukh D. Yu., Gordey I. S. Directions, methods and results of rye (Secale cereale L.) breeding in Belarus. Izvestiya Natsional'noy akademii nauk Belarusi. Seriya agrarnykh nauk = Proceedings of the National Academy of Sciences of Belarus. Agrarian series. 2022;60(2):160–170. (In Belarus). DOI: https://doi.org/10.29235/1817-7204-2022-60-2-160-170

12. Shchekleina L. M., Sheshegova T. K., Utkina E. I. Immunological structure and yield of winter rye varieties bred by the Federal Agricultural Research Center of the North-East named N. V. Rudnitsky. Agrarnaya nauka Evro-SeveroVostoka = Agricultural Science Euro-North-East. 2024;25(2):172–180. (In Russ.). DOI: https://doi.org/10.30766/2072-9081.2024.25.2.172-180

13. Miedaner T., Laidig F. Hybrid breeding in rye (Secale cereale L.). In Al‐Khayri J. M., Jain S. M., Johnson D. V. (Eds.), Advances in plant breeding strategies: Сereals. Springer, Cham, 2019. Pp. 343–372. DOI: https://doi.org/10.1007/978-3-030-23108-8_9

14. Volkova G. V., Yakhnik Ya. V., Zhukovskiy A. G. Sensitivity of the north caucasian and belarusian populations of Microdochium nivale (Fr.) Samuels & Hallet to fungicides. Sibirskiy vestnik sel'skokhozyaystvennoy nauki = Siberian Herald of Agricultural Science. 2022;52(6):42–50. (In Russ.). DOI: https://doi.org/10.26898/0370-8799-2022-6-5

15. Pronczuk M., Madej L. J. Evaluation of Microdochium nivale infection on rye genotypes using different methods. Vortraege fuer Pflanzenzuechtung (Germany). 1996;35:190–192.

16. Kasynkina O. M., Orlova N. S., Kanevskaya I. Yu. Evaluation of winter varieties of triticale on disease resistance. Agrarnyy nauchnyy zhurnal = The Agrarian Scientific Journal. 2017;(8):7–10. (In Russ.). URL: https://elibrary.ru/item.asp?id=29907362

17. Kruse E. B., Carle S. W., Wen N., Skinner D. Z., Murray T. D., Garland-Campbell K. A., Carter A. H. Genomic regions associated with tolerance to freezing stress and snow mold in winter wheat. G3: Genes, Genomes, Genetics. 2017;7(3):775–780. DOI: https://doi.org/10.1534/g3.116.037622

18. Miedaner T., Höxter H., Geiger H. H. Development of a resistance test for winter rye to snow mold (Microdochium nivale) under controlled environment conditions in regard to field inoculations. Canadian Journal of Botany. 1993;71(1):136–144. DOI: https://doi.org/10.1139/b93-015

19. Aamlid T. S., Landschoot P. J., Huff D. R. Tolerance to simulated ice encasement and Microdochium nivale in USA selections of greens-type Poa annua. Acta Agriculturae Scandinavica Section B–Soil and Plant Science. 2009;59(2):170–178. DOI: https://doi.org/10.1080/09064710802093854

20. Ponomareva M., Gorshkov V., Ponomarev S., Mannapova G., Askhadullin D., Askhadullin D., et al. Resistance to snow mold as a target trait for rye breeding. Plants. 2022;11(19):2516. DOI: https://doi.org/10.3390/plants11192516


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For citations:


Ponomareva M.L., Ponomarev S.N., Mannapova G.S., Pavlova S.Yu., Ivanova I.O. Optimization of methods for assessing the resistance of winter rye to snow mold in natural and artificial infection backgrounds. Agricultural Science Euro-North-East. 2025;26(3):564-576. (In Russ.) https://doi.org/10.30766/2072-9081.2025.26.3.564-576

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ISSN 2072-9081 (Print)
ISSN 2500-1396 (Online)