Effect of salicylic acid on productive traits of regenerated barley lines under soil stress
https://doi.org/10.30766/2072-9081.2025.26.5.1007-1014
Abstract
Cell selection is actively used to create genotypes of grain crops resistant to edaphic stressors. It is necessary to evaluate the genotypes selected in vitro under in vivo conditions to confirm the genetic stability of the target trait. To increase the yield of regenerated genotypes, it is possible to use growth regulators with adaptogenic and protective effects. In the conditions of a vegetation experiment, a comparative analysis of the productive traits of ‘Boyarin’ barley (Hordeum vulgare L.) cultivar and its regenerated R1-generation lines was carried out on soil backgrounds with drought (рНКCl – 6.5; artificially created water deficit), aluminum toxicity (рНКCl – 4.8; Al – 13 mg/kg) and cadmium (рНКCl – 4.7; Cd – 6.4 mg/kg), also without stress (control). The efficiency of barley treatment with salicylic acid (SA) (2 mM/dm3) as a stress protector under unfavorable soil conditions was assessed. The contribution of factors to the formation of barley yield was determined: soil background – 30.4 %, genotypic features – 8.1 %, treatment with salicylic acid – 0.7 %. Without SA treatment, the highest yield (130 g/m2) under stressful (drought) conditions was shown by regenerants obtained on selective media in vitro with water deficiency – RA-PEG. The stressprotective effect of SA was revealed against the background of cadmium in the regenerants RA-Cd and RA-PEG, the yield excess was more than twice over the original cultivar, amounting to 59 and 47 g/m2, and in the absence of stress factors – in the regenerants RA-Al and RA-PEG by 29 and 24 g/m2 , respectively. The regenerated line RA-PEG is recommended for use in breeding programs to create drought-resistant genotypes.
Keywords
Hordeum vulgare L.,
original cultivar,
regenerant,
soil background,
aluminum acidity,
cadmium toxicity,
drought,
stress protector,
productivity
Supporting agencies: The research was carried out under the support of the Ministry of Science and Higher Education of the Russian within the state assignment of the Federal Agricultural Research Center of the North-East named N. V. Rudnitsky (theme No. FNWE-2025-0008, 2022-0007).
About the Authors
O. N. Shupletsova
Federal Agricultural Research Center of the North -East named N. V. Rudnitsky
Russian Federation
Russian Federation
Olga N. Shupletsova, DSc in Biological Science, associate professor, leading researcher, the Laboratory of Biotechnological Methods of Agricultural Plant Breeding,
Lenin Str., 166a, Kirov, 610007
I. N. Shchennikova
Federal Agricultural Research Center of the North -East named N. V. Rudnitsky
Russian Federation
Russian Federation
Irina N. Shchennikova, DSc in Agriculture, corresponding member of the RAS, Head of the Laboratory of Breeding and Primary Seed Production of Barley,
Lenin Str., 166a, Kirov, 610007
S. N. Shevchenko
Samara Scientific Research Agriculture Institute named after N. M. Tulaykov – Branch of the Samara Federal Research Scientific Center of Russian Academy of Sciences
Russian Federation
Russian Federation
Sergey N. Shevchenko, DSc in Agriculture, academician of the RAS, Head of the Breeding Department,
41, Karl Marx St., village Bezenchuk, Samara region, 446254
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For citations:
Shupletsova O.N., Shchennikova I.N., Shevchenko S.N. Effect of salicylic acid on productive traits of regenerated barley lines under soil stress. Agricultural Science Euro-North-East. 2025;26(5):1007-1014. (In Russ.) https://doi.org/10.30766/2072-9081.2025.26.5.1007-1014
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