Varietal identification of soybeans by statistical parameters of light absorption
https://doi.org/10.30766/2072-9081.2025.26.6.1422-1430
Abstract
The statistical parameters of the effective light absorption spectra of soybean seeds of various cultivars and ripeness groups were studied. The purpose of the research was to identify the varietal features and to develop an algorithm for varietal identification of soybeans according to the statistical parameters of absorption spectra during photoluminescence excitation. Spectral absorption characteristics were obtained using a CM2203 diffraction spectrofluorimeter in the wavelength range of λ = 230–600 nm. The statistical parameters of the spectra were calculated – mathematical expectation, variance, skewness and kurtosis. Effective light absorption during photoluminescence excitation occurs in the range from 300 nm to 550 nm with the main maxima at 420nm, 390 and 362 nm. Radiation absorption is caused by the presence of phenolic acids, carotenoids, riboflavin, as well as terpenoids, sporopollenin, lipofuscin, lignin, or flavonoids. The mathematical expectation and variance are determined with a relatively small relative error – no more than 1.2 and 7.6 %, respectively, and the errors in determining skewness and kurtosis can reach 16.1–22 %. By the magnitude of the asymmetry, the ‘Barguzin’ cultivar can be uniquely identified. The remaining studied cultivars can be identified with varying probability by all four statistical parameters. The ‘Vilana’ and ‘Vilana beta’ cultivars differ significantly in terms of variance. An algorithm for varietal identification based on the probabilistic identity of soybeans to known cultivars has been developed and a database of statistical parameters of soybean cultivars has been obtained. To increase accuracy, a combination of statistical and previously obtained integral absorption parameters is possible. In the future it is planned to increase the number of identified cultivars, clarify the ranges and develop an optoelectronic soybean varietal identification system.
Keywords
Supporting agencies: the research was carried out under the support of the Ministry of Science and Higher Education of the Russian Federation within the state assignment of the Federal Scientific Agroengineering Center VIM (theme No. FGUN-2025-0007).
About the Author
M. V. Belyakov
Federal Scientific Agroengineering Center VIM
Russian Federation
Russian Federation
Mikhail V. Belyakov, DSc in Engineering, chief researcher
1st Institutsky proezd, 5, Moscow, 109428
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Review
For citations:
Belyakov M.V. Varietal identification of soybeans by statistical parameters of light absorption. Agricultural Science Euro-North-East. 2025;26(6):1422-1430. (In Russ.) https://doi.org/10.30766/2072-9081.2025.26.6.1422-1430
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