Use of growth regulators and experimental LED phytoirradiator in clonal micropropagation of garden strawberry (Fragaria × ananassa, Duchesne ex Weston)

The article provides experimental data of 2017-2018 study on the effect of growth regulators and LED phytoirradiator on the proliferation and rooting of promising garden strawberry (Fragaria ananassa) varieties in vitro. Micro-shoots of Korona and Brighton strawberry varieties were taken as the object of the research. Strawberry micro-shoots were cultivated under fluorescent lamps in the control variant. A programmable combined blinking LED phytoirradiator was under study. The combined effect of cytokinin and gibberellic acid by adding them to the Murashige and Skoog nutrient medium, as well as the impact of Siliplant and EcoFus growth regulators on strawberry micropropagation has been studied. It was established that in the cultivation of Korona variety the combined use of Siliplant and EcoFus under illumination with LED phytoirradiator provided an increase in the reproduction factor. The coefficient was 5.0 pcs./explant that was 1.7 times higher than the control (3.0 pcs/explant), the LSD05 1.4 pcs/explant. The maximum reproduction factor of remontant strawberry Brighton variety was obtained in the variant with the use of Siliplant and LED phytoirradiator and amounted to 4.9 pcs./explant (4.2 pcs./explant in the control), the LSD05 was 1.5 pcs./ explant. Regardless of the lighting, the use of RibavExtra in all variants under study increased the rooting rate of the strawberry Korona micro-shoots from 92.8 to 99.1%, the LSD05 6.1%. The use of LED phytoirradiator in comparison with the luminescent one (94.3%) provided a significant increase in the rooting rate of the strawberry Korona micro-shoots to 98.1% regardless of the growth regulators used, the LSD05 3.5%. The combined use of LED phytoirradiator and Ribav-Extra growth regulator in concentrations of 1.0 and 1.5 mg/l resulted in rooting of strawberry Korona micro-shoots up to 100%. Regardless of the growth regulator used, the use of LED phytoirradiator in comparison with the luminescent one (88.9%) provided a significant increase in the rooting rate of the strawberry Brighton micro-shoots to 97.2%, the LSD05 4.6%. The rooting rate of the remontant strawberry Brighton microshoots was 100% in the variant with the use of Ribav-Extra in the concentration of 1.0 mg /l combined with LED phytoirradiator 20 days after transplanting for rooting.

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