Features of lysine inheritance in grain sorghum hybrids of the second generation
https://doi.org/10.30766/2072-9081.2020.21.3.273-282
Abstract
Sorghum is one of the most important grain crops in the world. Sorghum grain is characterized by a low percentage of essential amino acids in protein, primarily lysine, which significantly reduces its feed value. There are two known mutant genes with a large lysine percentage, namely the spontaneous mutant hl gene, which was originally identified in Ethiopian lines, and the P721 gene, which is induced by ethylmethanesulfonate. The purpose of the current study was to identify the patterns of inheritance of the lysine percentage in grain sorghum hybrids of the second generation which were obtained by hybridization according to two 4x4 diallelic schemes (I - SPZS-11, Sb-126/4, 144 f/8, Zernogradskoe 204; II - ZSK-4, Belozernoe 100, Otbor 100, 34045). The hybridological analysis has established that in lysine percentage in sorghum protein there are genetic differences of 1-3 genes between the parental samples included in the hybridization. The greatest differences (3 genes) were identified between sorghum grain samples 34045 and 144 f/8. In most hybrid combinations there have been identified monogenic and digenic differences between the samples involved in hybridization. The estimation of dominance degree has identified the value of the gene manifestation, that controls lysine percentage in sorghum protein. There has been determined the dominance of both large and small values of the trait. The frequency distribution curves of the studied hybrids were within the variability of the parental forms, however, there were positive transgressions in some combinations. In the combinations Sb-126/4 × SPZS-11, Zernogradskoe 204 × SPZS-11 there have been identified the forms with a large lysine percentage in grain (more than 3.5%) for further breeding process.
Keywords
Supporting agencies: The research was carried out within the state assignment of the Agricultural Research Center “Donskoy” (theme No. 0706-2019-0001).
About the Authors
A. V. Alabushev
Agricultural Research Center “Donskoy”
Russian Federation
Russian Federation
Andrey V. Alabushev, academician of RAS, Doctor of Agricultural Sciences, professor, director
Nauchny Gorodok, 3, Zernograd, Zernogradsky district, Rostov region, 347740
V. V. Kovtunov
Agricultural Research Center “Donskoy”
Russian Federation
Russian Federation
Vladimir V. Kovtunov, PhD in Agricultural science, leading researcher, the Laboratory of Grain Sorghum Breeding and Seed Production
Nauchny Gorodok, 3, Zernograd, Zernogradsky district, Rostov region, 347740
P. I. Kostylev
Agricultural Research Center “Donskoy”
Russian Federation
Russian Federation
Pavel I. Kostylev, Doctor of Agricultural Science, professor, chief researcher, the Laboratory of Rice Breeding and Seed Production
Nauchny Gorodok, 3, Zernograd, Zernogradsky district, Rostov region, 347740
N. A. Kovtunova
Agricultural Research Center “Donskoy”
Russian Federation
Russian Federation
Nataliya A. Kovtunova, PhD in Agricultural science, leading researcher, the Laboratory of Forage Sorghum Breeding and Seed Production
Nauchny Gorodok, 3, Zernograd, Zernogradsky district, Rostov region, 347740
N. S. Kravchenko
Agricultural Research Center “Donskoy”
Russian Federation
Russian Federation
Nina S. Kravchenko, PhD in Biological science, senior researcher, the Laboratory of Biochemical Estimation of reeding Material and Seed Quality
Nauchny Gorodok, 3, Zernograd, Zernogradsky district, Rostov region, 347740
References
1. Tesso T., Ejeta G., Chandrashekar A., Huang C., Tandjung A., Lewamy M., Axtell J. D., Hamaker B. R. A Novel Modified Endosperm Texture in a Mutant High-Protein Digestibility/High-Lysine Grain Sorghum (Sorghum bicolor (L.) Moench). Cereal Chem., 2006;83(2):194-201. DOI: https://doi.org/10.1094/CC-83-0194
2. Taylor J. R. N., Schober T. J., Bean S. R. Novel food and non-food uses for sorghum and millets. J Cer Sci., 2006;(44(3)):252-271. DOI: https://doi.org/10.1016/j.jcs.2006.06.009
3. Vasil'chenko S. A., Metlina G. V., Kovtunov V. V. Vliyanie meteousloviy na produktivnost' sorgo zernovogo v yuzhnoy zone Rostovskoy oblasti. [The effect of weather conditions on grain sorghum productivity in the southern zone of the rostov region]. Politematicheskiy setevoy elektronnyy nauchnyy zhurnal Kubanskogo gosudarstvennogo agrarnogo universiteta = Polythematic online scientific journal of Kuban State Agrarian University. 2016;(120):744-754. (In Russ.). URL: https://www.elibrary.ru/item.asp?id=26469981
4. Romanyukin A. E., Shishova E. A., Kovtunova N. A., Ermolina G. M. Priznakovaya i geneticheskaya kollektsiya skorospelykh form sakharnogo sorgo. [Trait and genetic collection of the early maturing forms of sweet sorghum]. Agrarnyy vestnik Urala = Agrarian Bulletin of the Urals. 2016;7(149):46-50. (In Russ.). URL: https://www.elibrary.ru/item.asp?id=27191817
5. Mofokeng A. M., Shimelis H., Laing M. Breeding strategies to improve sorghum quality. AJCS. 2017;11(02):142-148. DOI: https://doi.org/10.21475/ajcs.17.11.02.p127
6. Kovtunov V. V. Posevnaya ploshchad' i urozhaynost' sorgo zernovogo. [Sown area and productivity of grain sorghum]. Zernovoe khozyaystvo Rossii = Grain Economy of Russia. 2018;(3):47-49. DOI: https://doi.org/10.31367/2079-8725-2018-57-3-47-49
7. Shepel' N. A. Selektsiya i semenovodstvo gibridnogo sorgo. [Breeding and seed production of hybrid sorghum]. Rostov-on-Don: Izdatel'stvo Rostovskogo universiteta, 1985. 256 p.
8. Shepel' N. A. Sorgo. [Sorghum]. Volgograd: Komitet po pechati, 1994. 448 p.
9. Ferreira R. R., Meinhardt L. W., Azevedo R. A. Lysine and threonine biosynthesis in sorghum seeds: Characterization of aspartate kinase and homoserine dehydrogenase isoenzymes. Ann Appl Biol. 2006;149(1):77-86. https://doi.org/10.1111/j.1744-7348.2006.00074.x
10. Wu Y., Yuan L., Guo X., Holding D. R., Messing J. Mutation in the seed storage protein kafirin creates a highvalue food trait in sorghum. Nat Commun. 2013;(4):2217. DOI: https://doi.org/10.1038/ncomms3217
11. Lushpina O. A., Sukhenko N. N., Kovtunov V. V. Kollektsionnyy material sorgo zernovogo dlya selektsii na produktivnost'. [The collection material of grain sorghum for the selection on productivity]. Zernovoe khozyaystvo Rossii = Grain Economy of Russia. 2016;(3):13-16. (In Russ.). URL: https://www.zhros.ru/jour/article/view/163
12. Singh R., Axtell J. D. High lysine mutant gene (hl) that improves protein quality and biological value of grain sorghum. Crop Sci. 1973;13(5):535-539. URL: https://acsess.onlinelibrary.wiley.com/doi/epdf/10.2135/cropsci1973.0011183X001300050012x
13. Fano D. Breeding of sorghum for high lysine in the seed. International Journal of Agriculture Sciences. 2017;9(43):4702-4707. URL: http://www.researchgate.net/publication/327384832
14. Fantaye B. M. Genetic improvement of lysine content in sorghum: A Review. J. Advan. Plant Sci., 2018;1(3):307. URL: https://www.researchgate.net/publication/329191838
15. Oria M. P., Hamaker B. R., Axtel J. D., Huang C. P. A highly digestible sorghum mutant cultivar exhibits a unique folded structure of endosperm protein bodies. Proceedings of the National Academy of Sciences, 2000;97(10):5065-5070. DOI: https://doi.org/10.1073/pnas.080076297
Review
For citations:
Alabushev A.V., Kovtunov V.V., Kostylev P.I., Kovtunova N.A., Kravchenko N.S. Features of lysine inheritance in grain sorghum hybrids of the second generation. Agricultural Science Euro-North-East. 2020;21(3):273-282. (In Russ.) https://doi.org/10.30766/2072-9081.2020.21.3.273-282
Views:
540
Email this article 