Environmental test of improved in vitro potato varieties in the conditions of Central Yakutia

Ecological tests of improved in vitro plants - potato regenerants of promising and zoned in Central Yakutia varieties have been carried out on cryogenic flood plain fibrous saline soils. To conduct a field experiment, clones adapted to the soil (2019) and their tuberous offspring (2020) were planted at the end of May. It was revealed that the most resistant to open ground conditions were regenerants of the Krasavchik variety (survival rate - 28.0 %); the least adapted variety was Vasilek (1.0 %). It has been established that among improved zoned varieties the most tolerant to arid conditions of vegetation (hydrothermal coefficient 0.4) and more productive (average tuber weight 213.9 g) was the Adretta variety. High index of tuber safety (100.0±5.0 %) was observed in Adretta variety (37 pcs.) and promising varieties Riviera (18 pcs.), Triumf (40 pcs.) and Rodrigo variety (16 pcs., 94.1±4.7 %). Zoned variety Vasilek (12 pcs., 80.0±4.0 %) and promising variety Assol (77.3±3.9 %, 17 pcs.) had no statistical difference in these indexes. The safety of Krasavchik variety tubers was 69.1±4.0 % (241pcs.). Zoned variety Rozard (65 pcs., 55.6±2.8 %) and promising Ilona variety had the same tuber safety. Minimum level of tuber safety was observed in Velina variety (18 pcs., 33.3±1.7 %).

1. Smirnov N. A. Znachimost' kartofelevodstva v agrarnoy ekonomike i obespechenii prodovol'stvennoy nezavisimosti regiona. [The importance of potato in the agricultural economy and ensuring food independence of the region]. Azimut nauchnykh issledovaniy: ekonomika i upravlenie = Azimuth of scientific research: economics and administration. 2016;5(3(16)):183-188. (In Russ.). URL: https://www.elibrary.ru/item.asp?id=27198795

2. Artyukhova S. I., Kirgizova I. V. Biotekhnologiya ozdorovleniya sibirskogo kartofelya ot virusov. [Biotechnology for the recovery of Siberian potatoes from viruses]. Omsk: izd-vo OmGTU, 2015. 136 с. URL: https://www.elibrary.ru/item.asp?id=26197993

3. Korshunov A. V., Simakov E. A., Lysenko Yu. N., Anisimov B. V., Mityushkin A. V., Gaitov M. Yu. Aktual'nye problemy i prioritetnye napravleniya razvitiya kartofelevodstva. [Actual problems and priority directions of innovative development of potato breeding]. Dostizheniya nauki i tekhniki APK = Achievements of Science and Technology of AICis. 2018;32(3):12-20. (In Russ.). DOI: https://doi.org/10.24411/0235-2451-2018-10303

4. Barsukova E. N., Kim I. V., Chekushkina T. N. Ozdorovlenie i mikrorazmnozhenie in vitro sortov kartofelya dlya bezvirusnogo semenovodstva. [Improvement and in vitro micropropagation of potato varieties for disease-free seed growing]. Dal'nevostochnyy agrarnyy vestnik = Far Eastern Agrarian Herald. 2018;(4(48)):20-26. (In Russ.). DOI: https://doi.org/10.24411/1999-6837-2018-14076

5. 5 Uskov A. I. Vosproizvodstvo ozdorovlennogo iskhodnogo materiala dlya semenovodstva kartofelya: 3. Razmnozhenie iskhodnykh rasteniy. [Reproduction of basic material for seed potato production: 3. basic plants propagation]. Dostizheniya nauki i tekhniki APK = Achievements of Science and Technology of AICis. 2009;(12):17-20. (In Russ.). URL: https://www.elibrary.ru/item.asp?id=14873175

6. Butenko R. G. Kul'tura izolirovannykh tkaney i fiziologiya morfogeneza rasteniy. [Isolated tissue culture and physiology of plant morphogenesis]. Moscow: Nauka, 1964. 272 p.

7. Kalinin F. L., Sarnatskaya V. V, Polishchuk V. E. Metody kul'tury tkaney v fiziologii i biokhimii rasteniy. [Tissue culture methods in plant physiology and biochemistry]. Kiev: Naukova dumka, 1980. 488 p.

8. Antonova O. Yu., Apalikova O. V., Ukhatova Yu. V., Krylova E. A., Shuvalov O. Yu., Shuvalova A. R., Gavrilenko T. A. Ozdorovlenie mikrorasteniy trekh kul'turnykh vidov kartofelya (Solanum tuberosum L., S. phureja Juz. & Buk. i S. stenotomum Juz. & Buk.) ot virusov metodom kombinirovannoy termo-khimioterapii. [Eradication of viruses in microplants of three cultivated potato species (Solanum tuberosum L., S. phureja Juz. & Buk., S. stenotomum Juz. & Buk.) using combined thermochemotherapy method]. Sel'skokhozyaystvennaya biologiya = Agricultural Biology. 2017;52(1):95-104. (In Russ.). DOI: https://doi.org/10.15389/agrobiology.2017.1.95rus

9. Cassells A. C., Long R. D. The elimination of potato viruses X, Y, S and M in meristem and explant cultures of potato in the presence of Virazole. Potato Res. 1982;25:165-173. DOI: https://doi.org/10.1007/BF02359803

10. Faccioli G., Loebenstein G., Berger P. H., Brunt A. A., Lawson R. H. Control of potato viruses using meristem and stem-cutting cultures, thermotherapy and chemotherapy. In: Virus and virus-like diseases of potatoes and production of seedpotatoes. Springer Netherlands, 2001. pp. 365-390. DOI: https://doi.org/10.1007/978-94-007-0842-6_28

11. Kornova K., Michailova J. Optimizing the rooting process in propagation of kazanlak oil-bearing rose (Rosa damascena Mill.) in vitro. Propagation of Ornamental Plants. 2008;8(4):224-229. URL: http://www.journal-pop.org/2008_8_4_224-229.html

12. Thomas P., Prabhakara B. S., Pitchaimuthu M. Cleansing the long-term micropropagated triploid watermelon cultures from covert bacteria and field testing the plants for clonal fidelity and fertility during the 7-10 year period in vitro. Plant Cell, Tissue and Organ Culture. 2006;85:317-329. DOI: http://dx.doi.org/10.1007/s11240-006-9083-5

13. Tyukavin G. B., Shmykova N. A., Monakhova M. A. Cytological study of embryogenesis in cultured carrot anthers. Russian journal of Plant physiology. 1999;46(6):767-773.

14. Bayrambekov Sh. B., Galkin A. N., Gar'yanova E. D. Ispol'zovanie rassady dlya polucheniya sverkhrannego urozhaya kartofelya. [Usage of seedlings for receiving ultraearly harvests of potato]. Nauchnyy zhurnal KubGAU. 2016;121(07):1-10. (In Russ.). DOI: https://doi.org/10.21515/1990-4665-121-084

15. Tekhnologiya vozdelyvaniya semennogo kartofelya v usloviyakh Yakutii: metodicheskie rekomendatsii. [Technology of cultivation of seed potatoes in the conditions of Yakutia: methodological recommendations]. Otv. red.: P. P. Okhlopkova. Sostaviteli: P. P. Okhlopkova, S. P. Efremova, N. S. Yakovleva, T. V. Sleptsova, M. N. Ivanova. RASKhN SO, Yakutskiy NIISKh. Yakutsk, 2010. 56 p. URL: https://e.nlrs.ru/online/view/14004/files/assets/basic-html/page-4.html#