Enzymative activity of technogenic surface formations of Kuzbass
https://doi.org/10.30766/2072-9081.2022.23.4.538-547
Abstract
The coal mining is one of the leading industries in the global energy balance. Kuzbass is the main region of Russia that specializes in coal mining. About 60 % of the country's coal is mined there. Coal mining is carried out mainly by the open-pit method. As a result, some 178 thousand hectares ha of disturbed land are formed. Enzymatic activity is an indicator of soil self-repair. The aim of the work was to study the enzymatic activity of technogenic surface formations of the Kuzbass to assess their toxicity and further selection of destructor microorganisms, rhizobacteria and hyperaccumulator plants, which will be further used at the biological stage of recultivation. As objects of research, the samples of technogenic surface formations taken on the territory of the Barzassky and Mokhovsky coal dumps. Enzymatic activity of technogenic surface formations of dumps was: invertase – 2,24 and 2,12 mg of sucrose split 1 g soil in 1 h; nitrite reductase – 0,57 and 0,07 mg reduced NO2- per 1 g soil in 24 h; asparaginase – 71,22 and 60,63 mg NH3 per 1 g soil in 24 h, respectively. When studying the enzymatic activity, it was assumed that the native microflora uses low- and high-molecular hydrocarbons (alkanes, polycyclic aromatic hydrocarbons (PAH), etc.) as carbon sources. The content of mobile forms of heavy metals (HM) in the studied samples exceeds the maximum allowable concentration by 1,2-2,6 times. In the course of statistical analysis, it was revealed that the gross and mobile forms of zinc and copper are nitrite reductase inhibitors, the gross and mobile form of nickel is an asparaginase activator, in technogenic disturbed formations of the studied coal dumps. Nickel is also an invertase inhibitor in the Mokhovsky coal dump.
Supporting agencies: The work was carried out with the support of the Ministry of Education and Science of the Russian Federation (theme FZSR-2021-00).
About the Authors
A. S. Frolova
Kemerovo State University
Russian Federation
Russian Federation
junior researcher, the Laboratory of Phytoremediation of Technogenically Disturbed Ecosystems,
Krasnaya str., 6, Kemerovo, 650000
M. K. Pereverzeva
Kemerovo State University
Russian Federation
Russian Federation
junior researcher, the Laboratory of Phytoremediation of Technogenically Disturbed Ecosystems,
Krasnaya str., 6, Kemerovo, 650000
L. K. Asyakina
Kemerovo State University
Russian Federation
Russian Federation
PhD in Engineering, Head of the Laboratory of Phytoremediation of Technogenically Disturbed Ecosystems,
Krasnaya str., 6, Kemerovo, 650000
Yu. V. Golubtsova
Kemerovo State University
Russian Federation
Russian Federation
DSc in Engineering, vice-rector for the development of the property complex,
Krasnaya str., 6, Kemerovo, 650000
M. A. Osintseva
Kemerovo State University
Russian Federation
Russian Federation
PhD in Engineering, Head of the Department of Project Activity,
Krasnaya str., 6, Kemerovo, 650000
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For citations:
Frolova A.S., Pereverzeva M.K., Asyakina L.K., Golubtsova Yu.V., Osintseva M.A. Enzymative activity of technogenic surface formations of Kuzbass. Agricultural Science Euro-North-East. 2022;23(4):538-547. (In Russ.) https://doi.org/10.30766/2072-9081.2022.23.4.538-547
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