Association of genotypes of cows of the Kholmogory breed by beta-casein with milk productivity

The aim of the study is to identify the frequency of occurrence of various allelic variants and genotypes of beta-casein in cows of the Kholmogory breed and their relationship with dairy productivity. The tasks of the research are genotyping of cattle of the Kholmogory breed by the beta-casein locus and establishing its connection with qualitative and quantitative indicators of dairy productivity. As the objects for the research there were taken 150 cows of the 1st, 2nd and 3rd lactation. An allele-specific variant of the PCR method (AS-PCR) was used to identify A1 and A2 beta-casein. As the result it had been established that in the studied part of the herd, 23 % of animals had the A2A2 genotype, 43 % of animals had the A1A1 genotype and 34 % of animals had the A1A2 genotype. For 100 days of the first lactation, animals with A1A2 genotype showed the highest value in milk yield. Animals with A2A2 genotype for 305 days of lactation had the highest milk yield and the amount of milk protein, however, the difference was not statistically significant compared to the animals with A1A2 genotype. Genotype A1A1 has lower indicators by all the parameters studied, with a significant difference relative to genotypes A1A2 and A2A2. Thus, the study of CSN2 is a promising area of scientific research, and the results of the study of beta-casein genotypes can be used as a marker selection in improving the herds of the Kholmogory breed.

1. Prozherin V. P., Yaluga V. L. Kholmogory breed. Molochnoe i myasnoe skotovodstvo = Journal of Dairy and Beef Cattle Farming. 2020;(7):10. (In Russ.). URL: https://www.elibrary.ru/item.asp?id=44347183

2. Matyukov V. S., Zharikov Ya. A., Zinovieva N. A. Genetic history and value of the gene pool appearing holmogosky breed. Molochnoe i myasnoe skotovodstvo = Journal of Dairy and Beef Cattle Farming. 2018;(2):2-8. (In Russ.). URL: https://elibrary.ru/item.asp?id=34859644

3. Kalashnikova L. A., Khabibrakhmanova Ya. A., Bagal I. E., Yaluga V. L., Prozherin V. P. Assessment of polymorphism of complex genotypes CSN3, LGB, PRL, GH, LEP and dairy productivity in kholmogory cows. Molochnoe i myasnoe skotovodstvo = Journal of Dairy and Beef Cattle Farming. 2019;(2):14-17. (In Russ.). URL: https://www.elibrary.ru/item.asp?id=37304535

4. Valitov F. R. Association of the beta-casein gene polymorphism with milk yields of planned cow breeds in the Republic of Bashkortostan. Izvestiya Orenburgskogo gosudarstvennogo agrarnogo universiteta = Izvestia Orenburg State Agrarian University. 2017;(1(63)):207-209. (In Russ.). URL: https://www.elibrary.ru/item.asp?id=28767185

5. Andiç S., Ayaz R. M., Oğuz Ş. A1 milk and beta-casomorphin-7. Food Health. 2021;7(2):128-137. DOI: https://doi.org/10.3153/FH21014

6. Malarmathi M., Senthil K. T., Parthiban M., Muthuramalingam T. T. Using allele specifi c PCR in Kangeyam and Holstein Friesian crossbred cattle in Tamil Nadu. Indian journal of veterinary and animal sciences and research. 2014;(43):310-315. URL: https://www.researchgate.net/publication/301341851_ANALYSIS_OF_ss-CASEIN_GENE_FOR_A1_AND_A2_GENOTYPE_USING_ALELE_SPECIFIC_PCR_IN_KANGEY_AM_AND_HOLSTEIN_FRIESIAN_CROSSBRED_CATTLE_IN_TAMIL_NADU_NADU

7. Mumtaz S., Javed K., Dawood M., Imran M., Ali A., Ramzan N. β casein Polymorphism in Indigenous and Exotic Cattle Breeds of Pakistan. Pakistan Journal of Zoology. 2022;54(3):1451-1454. DOI: https://doi.org/10.17582/journal.pjz/20190307030303

8. He M., Sun J., Jiang Z. Q., Yang Y. X. Effects of cow's milk beta-casein variants on symptoms of milk intolerance in Chinese adults: a multicentre, randomised controlled study. Nutrition journal. 2017;16:72. DOI: https://doi.org/10.1186/s12937-017-0275-0

9. De Gaudry D. K., Lohner S., Schmucker C., Kapp P., Motschall E., Horrlein S., Roger C., Meerpohl J. J. Milk A1 β-casein and health-related outcomes in humans: a systematic review. Nutrition reviews. 2019:77(5):278-306. DOI: https://doi.org/10.1093/nutrit/nuy063

10. Ramakrishnan M., Eaton T., Sermet O., Savaiano D. A Single meal of milk containing A2 β-casein causes fewer symptoms and lower gas production than milk containing both A1 and A2 β-casein among lactose intolerant individuals. Current Developments in Nutrition. 2020;12(12):3855. DOI: https://doi.org/10.3390/nu12123855

11. Schettini G. P., Lambert S. M., Da Silva Souza B. M. P., Costa R. B., De Camargo G. M. F. Genetic potential of Sindhi cattle for A2 milk production. Animal Production Science. 2020;60(7):893-895. DOI: https://doi.org/10.1071/AN18677

12. Ping F., Xiaojing D., Tie G., Ruyun D., Hongxu C. Determination of A2 β-casein and total β-casein in cow milk and milk powder by capillary zone electrophoresis. Chinese Journal of Chromatography. 2020;38(6):722-729. URL: https://pubmed.ncbi.nlm.nih.gov/34213205/

13. Ganguly I., Gaur G. K, Singh U., Kumar S., Kumar S., Mann S. Beta-casein (CSN2) polymorphism in Ongole (Indian zebu) and Frieswal (HF× Sahiwal crossbred) cattle. Indian Journal of Biotechnology. 2013;12(2):195-198. URL: https://www.researchgate.net/publication/280059679_Beta-casein_CSN2_polymorphism_in_Ongole_Indian_zebu_and_Frieswal_HF_X_Sahiwal_crossbred_cattle