Dependence of blood coagulation activity on temperature in cows in vitro
https://doi.org/10.30766/2072-9081.2024.25.1.90-97
Abstract
The paper presents the results of a study on how temperature affects the coagulation and anticoagulation processes in the hemostasis system of cows. The purpose of this work was to study the coagulation parameters of the blood of Ayrshire cows with hypo-, normo- and hyperthermia "in vitro", as well as to evaluate the possibility of using a coagulometer to characterize the activity of hemostasis and use hemostatic reactions in cattle as models for human medicine. The study was carried out in the Vologda region. The following parameters of the coagulation profile were analyzed: thrombin time (TT), prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen and antithrombin III (AT-III) activity. Results showed that at 43 °C hypercoagulation of the blood occurred, which in clotting methods manifests itself as an acceleration of TT by 61 % (27 sec), PT by 29 % (10 sec), a prolongation of APTT by 38 % (25 sec) and a decrease in Antithrombin III activity by 51 % (5 sec) compared with normothermia. At 18 °C, hypocoagulation was detected, accompanied by an elongation of all indicators characterizing the rate of clot formation: TT by 10 % (5 sec), PT by 61 % (55 sec), APTT by 83 % (289 sec). Fibrinogen activity decreased by 82 % (106 sec), and AT-III activity increased by 85 % (56 sec) compared to normothermy. The correlation between hemostasis parameters and the temperature factor can be characterized as negative moderate (APTT), noticeable (TV, fibrinogen) and close (PT, AT-III). Using a medical coagulometer caused reaction rates to differ significantly from those occurring at the body temperature of these animals when studying the coagulation mechanisms of cows. This does not allow to recommend this device for an adequate assessment of hemostatic reactions in cattle.
Supporting agencies: The research was financially supported by the Russian Science Foundation (Grant No. 23-26-00115), https://rscf.ru/project/23-26-00115/.
About the Authors
L. L. Fomina
Vologda State Dairy Farming Academy by N. V. Vereshchagin
Russian Federation
Russian Federation
Lyubov’ L. Fomina, PhD in Biological Science, associate professor
Schmidt str., 2, Molochnoe, Vologda, 160555
D. I. Berezina
Vologda State Dairy Farming Academy by N. V. Vereshchagin
Russian Federation
Russian Federation
Dar’ya I. Berezina, PhD in Biological Science, associate professor
Schmidt str., 2, Molochnoe, Vologda, 160555
T. S. Kulakova
Vologda State Dairy Farming Academy by N. V. Vereshchagin
Russian Federation
Russian Federation
Tat’yana S. Kulakova, PhD in Agricultural Science, associate professor
Schmidt str., 2, Molochnoe, Vologda, 160555
K. E. Modanova
Vologda State Dairy Farming Academy by N. V. Vereshchagin
Russian Federation
Russian Federation
Karina E. Modanova, student
Schmidt str., 2, Molochnoe, Vologda, 160555
References
1. Buryakov N. P., Buryakova M. A., Aleshin D. E. Heat stress and feeding features of the dairy cattle. Rossiyskiy veterinarnyy zhurnal = Russian veterinary journal. 2016;(3):5–13. (In Russ.). URL: https://elibrary.ru/item.asp?id=26538900
2. Mandal D. K., Bhakat C., Dutta T. K. Impact of environmental factors on physiological adaptability, thermotolerance indices, and productivity in Jersey crossbred cows. International Journal of Biometeorology. 2021;65:1999–2009. DOI: https://doi.org/10.1007/s00484-021-02157-2
3. Burhans W. S., Rossiter Burhans C. A., Baumgard L. H. Invited review: Lethal heat stress: The putative pathophysiology of a deadly disorder in dairy cattle. Journal of Dairy Science. 2022;105(5):3716–3735. DOI: https://doi.org/10.3168/jds.2021-21080
4. Min L., Cheng J., Zhao S., Tian H., Zhang Y., Li S., Yang H., Zheng N., Wang J. Plasma-based proteomics reveals immune response, complement and coagulation cascades pathway shifts in heat-stressed lactating dairy cows. Journal of Proteomics. 2016;146:99–108. DOI: https://doi.org/10.1016/j.jprot.2016.06.008
5. Xu S., Miao H., Gong L., Feng L., Hou X., Zhou M., Shen H., Chen W. Effects of Different Hypothermia on the Results of Cardiopulmonary Resuscitation in a Cardiac Arrest Rat Model. Disease Markers. 2022;2022:2005616. DOI: https://doi.org/10.1155/2022/2005616
6. Wu J., Yuan W., Li J., Zhao Yo., Li J., Li Zh., Li Ch. Effects of Mild Hypothermia on Cerebral Large and Small Microvessels Blood Flow in a Porcine Model of Cardiac Arrest. Neurocritical Care. 2017;27:297–303. DOI: https://doi.org/10.1007/s12028-017-0395-6
7. Gruzdeva O. V., Bychkova E. E., Penskaya T. Yu., Kuz'mina A. A., Antonova L. V., Barbarash L. S. Comparative analysis of the hemostasiological profile in sheep and patients with cardiovascular pathology as the basis for predicting thrombotic risks during preclinical tests of vascular prostheses. Sovremennye tekhnologii v meditsine = Modern technologies in medicine. 2021;13(1):52–58. (In Russ.). DOI: https://doi.org/10.17691/stm2021.13.1.06
8. Nikolaev V. Yu., Shakhmatov I. I., Kiselev V. I., Vdovin V. M. Rat,s hemostasis system in different modes оf the single hyperthermical load. Sovremennye problemy nauki i obrazovaniya = Modern problems of science and education. 2014;(4):509. (In Russ.). URL: https://elibrary.ru/item.asp?id=22285841
9. Berezina D. I., Fomina L. L. influence of cortisol analogs on secondary hemostasis indices in Cyprinus carpio carp. Inland Water Biology. 2022;15(5):659–666. DOI: https://doi.org/10.1134/s1995082922050042
10. Münster A. M., Olsen A. K., Bladbjerg E. M. Usefulness of human coagulation and fibrinolysis assays in domestic pigs. Comparative Medicine. 2022;52(1):39–43.
11. Barkagan Z. S., Momot A. P. Diagnosis and controlled therapy of hemostasis disorders. Moscow: izd-vo OOO Mediko-tekhnologicheskoe predpriyatie «N'yudiamed-AO», 2008. 292 p.
12. Berkovskiy A. L., Sergeeva E. V., Suvorov A. V. Diagnosis of hemostasis disorders in animals. Veterinariya = Veterinary. 2018;(5):54–57. (In Russ.). URL: https://www.elibrary.ru/item.asp?id=34857914
13. Proctor E. A., Dineen S. M., Van Nostrand S. C., Kuhn M. K., Barrett C. D., Brubaker D. K., Yaffe M. B., Lauffenburger D. A., Leon L. R. Coagulopathy signature precedes and predicts severity of end‐organ heat stroke pathology in a mouse model. Journal of thrombosis and haemostasis. 2020;18(8):1900–1910. DOI: https://doi.org/10.1111/jth.14875
14. Bruchim Y., Kelmer E., Cohen A., Codner C., Segev G., Aroch I. Hemostatic abnormalities in dogs with naturally occurring heatstroke. Journal of Veterinary Emergency and Critical Care. 2017;27(3):315–324. DOI: https://doi.org/10.1111/vec.12590
15. Bruchim Y., Horowitz M., Aroch I. Pathophysiology of heatstroke in dogs–revisited. Temperature. 2017;4(4):356–370. DOI: https://doi.org/10.1080/23328940.2017.1367457
16. Bouchama A., Roberts G., Al Mohanna F., El-Sayed R., Lach B., Chollet-Martin S., Ollivier V., Al Baradei R., Loualich A., Nakeeb S., Eldali A., De Prost D. Inflammatory, hemostatic, and clinical changes in a baboon experimental model for heatstroke. Journal of Applied Physiology. 2005;98(2):697–705. DOI: https://doi.org/10.1152/japplphysiol.00461.2004
17. Leon L. R., Helwig B. G. Heat stroke: role of the systemic inflammatory response. Journal of applied physiology. 2010;109(6):1980–1988. DOI: https://doi.org/10.1152/japplphysiol.00301.2010
18. Thienel M., Müller-Reif J. B., Zhang Z., Ehreiser V., Huth J., Shchurovska K., Kilani B., Schweizer L., Geyer P. E., Zwiebel M., Novotny J., Lüsebrink E., Little G., Orban M., Nicolai L., El Nemr S., Titova A., Spannagl M., Kindberg J., Evans A. L., Mach O., Vogel M., Tiedt S., Ormanns S., Kessler B., Dueck A., Friebe A., Jørgensen P. G., Majzoub-Altweck M., Blutke A., Polzin A., Stark K., Kääb S., Maier D., Gibbins J. M., Limper U., Frobert O., Mann M., Massberg S., Petzold T. Immobility-associated thromboprotection is conserved across mammalian species from bear to human. Science. 2023;380(6641):178–187. DOI: https://doi.org/10.1126/science.abo5044
Review
For citations:
Fomina L.L., Berezina D.I., Kulakova T.S., Modanova K.E. Dependence of blood coagulation activity on temperature in cows in vitro. Agricultural Science Euro-North-East. 2024;25(1):90-97. (In Russ.) https://doi.org/10.30766/2072-9081.2024.25.1.90-97
Views:
239
Email this article 