The effect of adding filamentous green algae Cladophora to the diet of rabbits on their blood parameters
https://doi.org/10.30766/2072-9081.2024.25.6.1137-1146
Abstract
Crimea is an iodine-deficient region, so feeding rabbits with iodine-enriched feed is a necessary condition for ensuring their normal functioning and productivity on farms. The use of algae from saline water bodies high in iodine and other valuable components can help solve this problem. The filamentous green alga Cladophora was collected from a Crimean hypersaline lake, and then granules were obtained from it. They were used in experiments on young rabbits. In 2024 there was studied the effect of Cladophora supplements in the diet of young rabbits of the Californian breed, from the age after weaning until reaching a slaughter weight of 2.5–2.8 kg. Three experimental and one control group of rabbits were formed (10 animals each). In the experimental groups, 1 %, 0.5 or 0.25 % of Cladophora were added to the diet of rabbits. The supplements have led to significant changes in the biochemical composition of blood serum: the concentration of urease and alpha-amylase increased by 36.7 and 50.4 % (p = 0.01), respectively. The increase in alpha-amylase was significantly directly proportional to the amount of supplementation (p = 0.001). The content of total protein and albumin increased significantly (p = 0.001–0.01). Within normal limits, the content of erythrocytes and leukocytes increased significantly (p = 0.05). Thrombocrit also increased significantly (p = from 0.05 to 0.001). The data obtained showed that when Cladophora was added to the diet of rabbits, all characteristics of its blood did not go beyond the known normal limits, while positive changes occurred in a number of them. There is an intensification of metabolic processes and kidney enzymes. The results obtained as well as published data have shown that adding Cladophora to the diet of rabbits can be an effective feed additive that improves their physiological condition and enhances immunity.
About the Authors
N. V. ShadrinRussian Federation
Nickolai V. Shadrin, PhD in Biological Science, associate professor, leading researcher, the Laboratory of Extreme Ecosystems
2 Nakhimov ave, Sevastopol, 299011
P. S. Ostapchuk
Russian Federation
Pavel S. Ostapchuk, PhD in Agricultural Science, leading researcher, the Department of Field Crops
150, Kievskaya str, Simferopol, 295043
T. A. Kuevda
Russian Federation
Tatyana A. Kuevda, PhD in Biological Science, senior researcher, the Department of Field Crops
150, Kievskaya str, Simferopol, 295043
A. V. Prazukin
Russian Federation
Alexander V. Prazukin, DSc in Biological Science, leading researcher, the Laboratory of Extreme Ecosystems
2 Nakhimov ave., Sevastopol, 299011
Yu. K. Firsov
Russian Federation
Yuri K. Firsov, PhD in Biological Science, senior researcher, the Laboratory of Extreme Ecosystems
2 Nakhimov ave., Sevastopol, 299011
D. D. Gassiev
Russian Federation
Danil D. Gassiev, engineer, the Laboratory of Extreme Ecosystems
2 Nakhimov ave., Sevastopol, 299011
D. V. Zubochenko
Russian Federation
Denis V. Zubochenko, PhD in Biological Science, Deputy Director for Production and Implementation of Innovative Developments
150, Kievskaya str, Simferopol, 295043
E. V. Anufriieva
Russian Federation
Elena V. Anufriieva, DSc in Biological Science, Head of the Laboratory of Extreme Ecosystems
2 Nakhimov ave., Sevastopol, 299011
References
1. Liu X., Liu W., Tang Q., Liu B., Wada Y., Yang H. Global agricultural water scarcity assessment incorporating blue and green water availability under future climate change. Earth’s Future. 2022;10(4):e2021EF002567. DOI: https://doi.org/10.1029/2021EF002567
2. FAO. The state of world fisheries and aquaculture 2016. Contributing to food security and nutrition for all. Food and Agricultural Organization of the United Nations. Italy, Rome: 2016. 200 p. URL: https://www.fao.org/3/I5555E/i5555e.pdf
3. World Bank. World development report 2008. Agriculture for Development. Washington, DC, 2007. 390 p. DOI: https://doi.org/10.1596/978-0-8213-6807-7
4. Cordeiro M. R., Mengistu G. F., Pogue S. J., Legesse G., Gunte K. E., Taylor A. M., et al. Assessing feed security for beef production within livestock–intensive regions. Agricultural Systems. 2022;196:103348. DOI: https://doi.org/10.1016/j.agsy.2021.103348
5. Wu G. Nutrition and metabolism: foundations for animal growth, development, reproduction, and health. Advances in Experimental Medicine and Biology. 2022;1354:1–24. DOI: https://doi.org/10.1007/978-3-030-85686-1_1
6. Dawson I. K., Attwood S. J., Park S. E., Jamnadass R., Powell W., Sunderland T., et al. Contributions of biodiversity to the sustainable intensification of food production. Thematic study for The State of the World’s Biodiversity for Food and Agriculture. FAO, Rome, 2019. pp. 23–37. URL: https://openknowledge.fao.org/server/api/core/bitstreams/229138c2-f17f-43ae-a15a-1b73a45e7277/content
7. Prazukin A. V., Anufriieva E. V., Shadrin N. V. Is biomass of filamentous green algae Cladophora spp. (Chlorophyta, Ulvophyceae) an unlimited cheap and valuable resource for medicine and pharmacology? A review. Reviews in Aquaculture. 2020;12(4):2493–2510. DOI: https://doi.org/https://doi.org/10.1111/raq.12454
8. Costa M., Cardoso C., Afonso C., Bandarra N. M., Prates J. A. Current knowledge and future perspectives of the use of seaweeds for livestock production and meat quality: a systematic review. The Journal of Animal Physiology and Animal Nutrition. 2021;105(6):1075–1102. DOI: https://doi.org/10.1111/jpn.13509
9. Anufriieva E. V. How can saline and hypersaline lakes contribute to aquaculture development? A review. Journal of Oceanology and Limnology. 2018;36:2002–2009. DOI: https://doi.org/10.1007/s00343-018-7306-3
10. Prazukin A. V., Anufriieva E. V., Shadrin N. V. Biomass of Cladophora (Chlorophyta, Cladophorales) is a promising resource for agriculture with high benefits for economics and the environment. Aquaculture International. 2024;23(3):3637–3673. DOI: https://doi.org/10.1007/s10499-023-01342-x
11. Ivanov C. V., Guk M. G., Fazilova F. R., Plisko E. F. Relationship of chemical composition of soil and surface waters of the republic of crimea and their influence on development of endemic diseases. Tsentral'nyy nauchnyy vestnik. 2018;3(10):15–19. (In Russ.). URL: https://elibrary.ru/item.asp?id=34998549
12. Karabaeva M. E. The problem of iodine deficiency in animals. Effektivnoe zhivotnovodstvo. 2018;2(141):28–29. (In Russ.). URL: https://elibrary.ru/item.asp?id=32735626
13. Zubochenko D., Pashtetsky V., Ostapchuk P., Kuevda T., Zyablitskaya Ye., Makalish T., Kopylova A. Effect of antioxidants in a liposomal form containing organic iodine of the blood serum biochemical composition and the structure of muscle tissue formation of young rabbits. EE3S Web of Conferences. 2020;224:04003. DOI: https://doi.org/10.1051/e3sconf/202022404003
14. Mantri V. A., Gajaria T. K., Rathod S. G., Prasad K. A Mini Review on Iodinophyte Seaweed Resources of India. Proceedings of the National Academy of Sciences, India, Section B: Biological Sciences. 2024:1–12. DOI: https://doi.org/10.1007/s40011-024-01571-x
15. Ferraz S., Ragonezi C., Nunes N., Valente S., Carvalho M. A. Different seaweeds use for iodine deficiency overcome. Biomedical – Journal of Scientific & Technical Research. 2019;15(3):11405–11407. DOI: https://doi.org/10.26717/BJSTR.2019.15.002710
16. Seraya O. Yu., Kvartnikova E. G. Non-traditional feed for rabbits and poultry. Effektivnoe zhivotnovodstvo. 2022;(7(182)):108–110. (In Russ.). DOI: https://doi.org/10.24412/cl-33489-2022-7-108-110
17. Zamaratskaia G., Havrysh O., Korzeniowska M., Getya A. Potential and limitations of rabbit meat in maintaining food security in Ukraine. Meat science. 2023;204:109293. DOI: https://doi.org/10.1016/j.meatsci.2023.109293
18. Nutautaitė M., Racevičiūtė-Stupelienė A., Bliznikas S., Pockevičius A., Vilienė V. River-sourced Cladophora glomerata macroalgal biomass as a more sustainable and functional feed raw material for growing rabbits. Italian Journal of Animal Science. 2024;23(1):607–617. DOI: https://doi.org/10.1080/1828051X.2024.2342380
19. Yushkov B. G., Korneva E. A., Chereshnev V. A. The concept of norm in physiology and pathophysiology. Physiological constants of laboratory animals. Ekaterinburg: UrO RAN, 2021. 864 p. URL: https://elib.usma.ru/handle/usma/4814
20. Voitenko N. G., Makarova M. N., Kovaleva M. A. Variability of blood biochemical parameters and establishment of reference intervals in preclinical studies. Part 2: rabbit. Laboratornye zhivotnye dlya nauchnykh issledovaniy = Laboratory Animals for Science. 2020;(2):3–10. (In Russ.). DOI: https://doi.org/10.29296/2618723X-2020-02-01
Review
For citations:
Shadrin N.V., Ostapchuk P.S., Kuevda T.A., Prazukin A.V., Firsov Yu.K., Gassiev D.D., Zubochenko D.V., Anufriieva E.V. The effect of adding filamentous green algae Cladophora to the diet of rabbits on their blood parameters. Agricultural Science Euro-North-East. 2024;25(6):1137-1146. (In Russ.) https://doi.org/10.30766/2072-9081.2024.25.6.1137-1146