Dynamics of progesterone concentration in the blood of cattle after the use of Progestamag^®

The aim of the research was to study the dynamics of progesterone concentration in the blood of heifers (n = 10) and lactating cows (n = 10) with signs of ovarian hypofunction after a single intramuscular injection of Progestamag® in a dosage of 10 ml. The experimental work was performed on animals of the Ayrshire breed. Ovarian hypofunction was determined by ultrasound sonography based on the absence of yellow bodies and large follicles. To determine the level of progesterone before administration of the drug and for 14 days after, venous blood was obtained from animals. The hormone concentration was determined by the enzyme immunoassay method. When calculating the pharmacokinetic parameters, the maximum concentration of the substance (Cmax), the time of onset of maximum concentration (Tmax), the half-life (T1/2), as well as the elimination constant (Kelim) were determined. Based on the obtained data, dose-dependent changes in progesterone concentration were calculated using a linear method. It was found that after a single injection of the drug, there was a sharp increase in the level of the studied steroid. In heifers, Tmax was observed on the second day, while Cmax was 42.88 nmol/L, Kelim was 0.0083, and T1/2 was 83.3 hours. A significantly high concentration of the hormone was observed for 9 days. The peak progesterone content in lactating cows also appeared after 48 hours, while the Cmax was 24.96 nmol/L, Kelim was 0.0079, and T1/2 was 88.1 hours. Dose-dependent calculations showed that for 7...10 day sexual stimulation regimens in cycling heifers, the therapeutic range was from 1 up to 4 ml, and in cows from 2 to 7 ml of the drug. For the treatment of gonadal hypofunction, a volume of 2 to 6 ml can be considered a reasonable dosage for heifers, and from 4 to 7 ml of the drug for lactating animals. Thus, Progestamag® has a moderately prolonged effect, however, a gradual decrease in the level of the steroid over a long period of time may hinder the achievement of the rebound effect.

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