Biobanks of genetic resources of farm animals worldwide and in Russia (review)

The decline in biodiversity and, as a consequence, in genetic resources of farm animals worldwide as a result of the growing anthropogenic impact is recognized as a global problem that requires an immediate solution. Therefore, the conservation of national gene pools of farm animals is of crucial importance. One of the most effective forms of conservation of genetic resources is the long-term deposit of animal biomaterials in specialized collections-biobanks. Global experience shows that the creation of such biobanks with materials accessible to scientists, breeders, and agricultural professionals, is of great scientific and applied importance. The article analyzes domestic and foreign sources over the last 10 years. The purpose of the review is to summarize current data on the development of biobanking of farm animals worldwide and in the Russian Federation. The current state of the problem of conservation of genetic resources of farm animals and approaches for its solution are considered, the main emphasis is on the conservation of materials in vitro. The paper describes the current state of collections of genetic resources of farm animals in the Russian Federation and the largest national gene banks in several countries. The need to expand work in the field of biobanking of farm animals is shown. In the future, biobank samples can be used both to preserve populations of small, rare animal breeds and to restore the genetic variability of commercial, actively bred breeds. With the development of omix technologies, biobanks of farm animals are becoming particularly important as sources of genetically diverse and specialized DNA that can be used to study gene functions and analyze genomic associations.

1. Astrelina T. A., Samoilov A. S. Features of the activity of biological banks: world practice. Klinichesky vestnik FMBTs im. A. I. Burnazyana = A. I. Burnasyan FMBC clinical bulletin. 2022;(2):45–48. (In Russ.). DOI: https://doi.org/10.33266/2782-6430-2022-2-45-48

2. Aripova T. U., Ruzibakiyeva M. R., Riskulov F. T. Biobanks and their importance in the modern world. Zhurnal teoreticheskoy i klinicheskoy meditsini = Journal of Theoretical and Clinical Medicine. 2024;(4):53–57. (In Uzbekistan). URL: https://elibrary.ru/item.asp?id=73875887

3. Harati M. D., Williams R. R., Movassaghi M., Hojat A., Lucey G. M., Yong W. H. An Introduction to Starting a Biobank. Biobanking. Methods in Molecular Biology. 2019;1897:7–16. DOI: https://doi.org/10.1007/978-1-4939-8935-5_2

4. Berezina G. M., Mirzakhmetova D. D., Svyatova G. S., Terlikbayeva A. T., Murtazaliyeva A. V. Organizatsiya biobanka DNK i yego rol v nauchnikh issledovaniyakh. Nauka i zdravookhraneniye = Science and Healthcare. 2021;23(4):208–217. (In Kazastan). DOI: https://doi.org/10.34689/SH.2021.23.4.023

5. Meshkov A. N., Yartseva O. Yu., Borisova A. L., Pokrovskaya M. S., Drapkina O. M. The concept of the national information platform of biobanks of the Russian Federation. Kardiovaskulyarnaya terapiya i profilaktika = Cardiovascular Therapy and Prevention. 2022;21(11):3417. (In Russ.). DOI: https://doi.org/10.15829/1728-8800-2022-3417

6. Balanovskaya E. V., Zhabagin M. K., Agdzhoyan A. T., Chukhryayeva M. I., Markina N. V., Balaganskaya O. A. et al. Population biobanks: organizational models and prospects of application in gene geography and personalized medicine. Genetika = Russian Journal of Genetics. 2016;52(12):1371–1387. (In Russ.). DOI: https://doi.org/10.7868/S001667581612002X

7. Groeneveld L. F., Gregusson S., Guldbrandtsen B., Hiemstra S. J., Hveem K., Kantanen J. et al. Domesticated Animal Biobanking: Land of Opportunity. PLoS Biology. 2016;14(7):e1002523. DOI: https://doi.org/10.1371/journal.pbio.1002523

8. LaLonde-Paul D., Mouttham L., Promislow D. E. L., Castelhano M. G. Banking on a new understanding: translational opportunities from veterinary biobanks. GeroScience. 2023;45(3):1439–1450. DOI: https://doi.org/10.1007/s11357-023-00763-z

9. Stolpovsky Yu. A., Piskunov A. K., Svishcheva G. R. Genomic selection. i: latest trends and possible ways of development. Genetika = Russian Journal of Genetics. 2020;56(9):1006–1017. (In Russ.). DOI: https://doi.org/10.31857/S0016675820090143

10. Delgado Bermejo J. V., Martinez Martínez M. A., Rodríguez Galvan G., Stemmer A., Navas Gonzalez F. J., Camacho Vallejo M. E. Organization and Management of Conservation Programs and Research in Domestic Animal Genetic Resources. Diversity. 2019;11(12):235. DOI: https://doi.org/10.3390/d11120235

11. Blackburn H. D. Biobanking genetic material for agricultural animal species. Annual review of animal biosciences. 2018;6(1):69–82. DOI: https://doi.org/10.1146/annurev-animal-030117-014603

12. Gutiérrez-Reinoso M. A., Aponte P. M., García-Herreros M. A review of inbreeding depression in dairy cattle: current status, emerging control strategies, and future prospects. Journal of Dairy Research. 2022;89(1):3–12. DOI: https://doi.org/10.1017/S0022029922000188

13. Djedovic R., Radojkovic D., Stanojevic D., Savic R., Vukasinovic N., Popovac M. et al. Base characteristics, preservation methods, and assessment of the genetic diversity of autochthonous breeds of cattle, sheep and pigs in Serbia: A Review. Animals (Basel). 2024;14(13):1894. DOI: https://doi.org/10.3390/ani14131894

14. Stanishevskaya O. I., Cherepanov S. V., Silyukova Yu. L. Organizational aspects of genetic resources preservation in farm animals: world experience. Genetika i razvedeniye zhivotnikh = Genetics and breeding of animals. 2017;(3):3–11. (In Russ.). URL: https://www.elibrary.ru/item.asp?id=32340644

15. Blackburn H. D., Lozada-Soto E., Paiva S. R. Biobanking animal genetic resources: critical infrastructure and growth opportunities. Trends in Genetics. 2024;40(2):115–117. DOI: https://doi.org/10.1016/j.tig.2023.11.004

16. Heimstra S. J., Martyniuk E., Duchev Z., Begemann F. European gene bank network for animal genetic resources (EUGENA). Proceedings, 10th World Congress of Genetics Applied to Livestock Production. Canada: Vancouver, 2014. 3 p. URL: https://www.asas.org/docs/default-source/wcgalp-posters/437_paper_8691_manuscript_289_0.pdf

17. Paiva S. R., McManus C. M., Blackburn H. Conservation of animal genetic resources – A new tact. Livestock Science. 2016;193:32–38. DOI: https://doi.org/10.1016/j.livsci.2016.09.010

18. Blackburn H. D., Wilson C. S., Krehbiel B. Conservation and utilization of livestock genetic diversity in the United States of America through gene banking. Diversity. 2019;11(12):244. DOI: https://doi.org/10.3390/d11120244

19. Blackburn H. D., Azevedo H. C., Purdy P. H. Incorporation of Biotechnologies into Gene Banking Strategies to Facilitate Rapid Reconstitution of Populations. Animals. 2023;13(20):3169. DOI: https://doi.org/10.3390/ani13203169

20. Ren Q., Gong Y., Su P., Liu G., Pu Y., Yu F. et al. How Developments in Genebanks Could Shape Utilization Strategies for Domestic Animals. Agriculture. 2025;15(2):133. DOI: https://doi.org/10.3390/agriculture15020133

21. Orth D., Ramos A. F., Carvalho G. M. C., Basílio L. M. S., Caetano A. R., Ianella P. Genomic characterization of the Brazilian CriouloLageano: Insights for conservation of a Brazilian local bovine breed. Livestock Science. 2024;284:105481. DOI: https://doi.org/10.1016/j.livsci.2024.105481

22. De Oliveira Silva R., Ahmadi B. V., Hiemstra S. J., Moran D. Optimizing ex situ genetic resource collections for European livestock conservation. Journal of Animal Breeding and Genetics. 2019;136(1):63–73. DOI: https://doi.org/10.1111/jbg.12368

23. Jenderek M. M., Ambruzs B. D., Yeater K. M., Reed B. M. Evaluating shoot-tip regrowth of 25 Rubus L. species and hybrids after 15 to 20 years of cryopreserved storage. Cryobiology. 2025;118:105159. DOI: https://doi.org/10.1016/j.cryobiol.2024.105159

24. Azevedo H. C., Blackburn H. D., Lozada-Soto E. A., Spiller S. F., Purdy P. H. Enhancing evaluation of bull fertility through multivariate analysis of sperm. Journal of Dairy Science. 2024;107(12):11774–11784. DOI: https://doi.org/10.3168/jds.2024-25163

25. McManus C., Hermuche P. M., Paiva S. R., Guimarães R. F., Junior O. A. C., Blackburn H. D. Gene bank collection strategies based upon geographic and environmental indicators for beef breeds in the United States of America. Livestock Science. 2021;254:104766. DOI: https://doi.org/10.1016/j.livsci.2021.104766

26. Rexroad C., Vallet J., Matukumalli L. K., Reecy J., Bickhart D., Blackburn H. et al. Genome to Phenome: Improving Animal Health, Production, and Well-Being - A New USDA Blueprint for Animal Genome Research 2018-2027. Frontiers in Genetics. 2019;10:327. DOI: https://doi.org/10.3389/fgene.2019.00327

27. Wang W., Feng C., Liu F., Li J. Biodiversity conservation in China: A review of recent studies and practices. Environmental Science and Ecotechnology. 2020;2:100025. DOI: https://doi.org/10.1016/j.ese.2020.100025

28. Deng H., Jin Y., Pray C., Hu R., Xia E., Meng H. Impact of public research and development and extension on agricultural productivity in China from 1990 to 2013. China Economic Review. 2021;70:101699. DOI: https://doi.org/10.1016/j.chieco.2021.101699

29. Chen Q., Fan C. T., Jiao H. The current status and improvement directions of legal rules regarding Chinese national gene banks for farm animal genetic resources. Frontiers in Genetics. 2024;15:1413625. DOI: https://doi.org/10.3389/fgene.2024.1413625

30. Machado L. C., Oliveira V. C., Paraventi M. D., Cardoso R. N., Martins D. S., Ambrósio C. E. Maintenance of Brazilian Biodiversity by germplasm bank. Pesquisa Veterinária Brasileira. 2016;36(1):62–66. DOI: https://doi.org/10.1590/S0100-736X2016000100010

31. Mariante A. D. S., do SM Albuquerque M., Egito A. D., McManus C., Lopes M. A., Paiva S. R. Present status of the conservation of livestock genetic resources in Brazil. Livestock Science. 2009;120(3):204–212. DOI: https://doi.org/10.1016/j.livsci.2008.07.007

32. Fonseca J. F., Silva K. D. M. Brazilian Germplasm Bank: conservation of genetic resources of sheep and goats. (Embrapa Caprinos e Ovinos. Documentos, 142). Sobral: Embrapa Caprinos e Ovinos, 2021. 25 p. URL: http://ainfo.cnptia.embrapa.br/digital/bitstream/item/230303/1/CNPC-2021-Art99.pdf

33. Ahmad S., Kour G., Singh A., Gulzar M. Animal genetic resources of India-an overview. International Journal of Livestock Research. 2019;9(3):1–12. DOI: https://doi.org/10.5455/ijlr.20181025013931

34. Vani A., Singh R., Tiwari V. K., Gangwar M., Mir M., Hussain P. C., Saini T. Conservation of animal genetic resources in India: The gene bank approach. The Pharma Innovation Journal. 2022;11(11S):1262–1264.

35. Chinarov V. I. Territorial development and transformation of the gene pool in Russian dairy cattle breeding. Molochnoye i myasnoye skotovodstvo = Journal of Dairy and Beef Cattle Farming. 2024;(4):7–12. (In Russ.). DOI: https://doi.org/10.33943/MMS.2024.63.86.002

36. Paronyan I. A. The current state of the gene pool of dairy and dairy-meat cattle breeds in the Russian Federation. Dostizheniya nauki i tekhniki APK = Achievements of Science and Technology of AICis. 2020;34(6):79–83. (In Russ.). DOI: https://doi.org/10.24411/0235-2451-2020-10615

37. Amerkhanov Kh. A., Koldayeva E. M. Problems of preserving breed diversity of farm animals in the Russian Federation. Izvestiya Mezhdunarodnoy akademii agrarnogo obrazovaniya = Izvestia MAAO. 2023;(65):158–161. (In Russ.). URL: http://maaorus.ru/assets/files/journals/izvestiya-maao-vypusk-65.pdf#page=158

38. Kalashnikov V. V., Zaytsev A. M., Lebedeva L. F. Genetic resources of russian horse breeding and biotechnological methods of their conservation. Konevodstvo i konny sport = Horse Breeding and Equestrian Sports. 2024;(3):4–9. (In Russ.). URL: DOI: https://doi.org/10.25727/HS.2024.3.60837

39. Babenkov V. Yu., Chimidova N. V., Khakhlinov A. I., Ubushiyeva A. V., Manzhiyev V. I. The role of reproductive biotechnologies in the reproduction and preservation of the gene pool of rare and endangered breeds of cattle. Zhivotnovodstvo i kormoproizvodstvo = Animal Husbandry and Fodder Production. 2023;106(1):67–76. (In Russ.). URL: https://www.elibrary.ru/item.asp?id=50753393

40. Stolpovsky Yu. A., Zakharov-Gezekhus I. A. The problem of conservation of gene pools of domesticated animals. Vavilovsky zhurnal genetiki i selektsii = Vavilov Journal of Genetics and Breeding. 2017;21(4):477–486. (In Russ.). URL: DOI: https://doi.org/10.18699/VJ17.266

41. Ulimbashev M. B., Kulintsev V. V., Selionova M. I., Ulimbasheva R. A., Abilov B. T., Alagirova Zh. T. Rational management of the gene pool of valuable breeds of animals for the purpose of conservation of biological diversity. Yug Rossii: ekologiya, razvitiye = South of Russia: ecology, development. 2018;13(2):165–183. (In Russ.). DOI: https://doi.org/10.18470/1992-1098-2018-2-165-183

42. Stolpovsky Yu. A., Beketov S. V., Solodneva E. V., Kuznetsov S. B. Conservation of genetic resources of farm animals. Glavny zootekhnik = Head of Animal Breeding. 2024;(3):3–18. (In Russ.). DOI: https://doi.org/10.33920/sel-03-2403-01

43. Alpeeva E. V., Sharova N. P., Sharov K. S., Vorotelyak E. A. Russian biodiversity collections: A professional opinion survey. Animals. 2023;13(24):3777. DOI: https://doi.org/10.3390/ani13243777

44. Milovanov V. K. Biology of reproduction and artificial insemination of animals. Moscow: Izd-vo s.-kh. literaturi, zhurnalov i plakatov, 1962. 696 p.

45. Koshkina O., Deniskova T., Dotsev A., Kunz E., Selionova M., Medugorac I., Zinovieva N. Phylogenetic analysis of Russian native sheep breeds based on mtDNA sequences. Genes. 2023;14(9):1701. DOI: https://doi.org/10.3390/genes14091701

46. Bagirov V. A., Zinovyeva N. A. The current state and world experience in the conservation of genetic resources of farm animals. Uspekhi nauk o zhivotnikh = Ernst Journal of Animal Science. 2024;(1):5–24. (In Russ.). URL: https://www.elibrary.ru/item.asp?id=82336444

47. Aibazov M. M., Mamontova T. V. The creation of bio-resource collections is the necessary condition for conservation and rational use of animal genetic resources. Selskokhozyaystvenny zhurnal. 2018;2(11):54–62. (In Russ.). DOI: https://doi.org/10.25930/47pm-n875

48. Gulov A. N., Berezin A. S., Larkina E. O., Saltykova E. S., Kaskinova M. D. Creation of a Biobank of the Sperm of the Honey Bee Drones of Different Subspecies of Apis mellifera L. Animals 2023;13(23):3684. DOI: https://doi.org/10.3390/ani13233684

49. Zinovyeva N. A., Fisinin V. I., Bagirov V. A., Kostyunina O. V., Gladir E. A. Bioresource centers as a form for conservation of animal genetic resources of agricultural purpose. Dostizheniya nauki i tekhniki APK = Achievements of Science and Technology of AICis. 2013;(11):40–41. (In Russ.). URL: https://www.elibrary.ru/item.asp?id=20678305