The effect of laboratory samples of new biological products and their mixtures with organomineral fertilizers on growth and development of winter wheat and sunflower plants

Currently, there is an active development of environmentally friendly methods of farming. However, the range of products for this type of farming system is limited. Growth stimulation is one of the mechanisms ensuring plant resistance to diseases that reduce crop yields. The aim of the work is to study the effect of laboratory samples of new biological products based on B. subtilis BZR 336 g, B. subtilis BZR 517, P. chlororaphis 245 F strains and organo-mineral fertilizers PRK White Pearl Brown (WPB) and PRK White Pearl Universal” (WPU) on plant growth and development of plants, as well as study of the possibility of their joint use for subsequent inclusion in organic farming systems or integrated plant protection. Growthpromoting ability was assessed in winter wheat and sunflower plants grown in sand in a climatic chamber. As comparison variants there were seed treatment with water (control), chemical (Dividend Star, KS) and biological (Fitosporin-M, F, B. subtilis 26 D) standards. In two plant cultures there was an expected retardant effect of the chemical standard and the growth-promoting effect of the biological standard. Statistically significant data were obtained for such a parameter as the shoot length. On winter wheat, laboratory samples provided the shoot length of 24.1-25.2 mm (an addition to the control of 3.4-8.2%), organic and mineral fertilizers - 23.9-24.5 mm (2.6-5.2%), mixtures with WPU - 23.2-24.4 mm (-0.4-4.7%), mixtures with WPB - 24.1-25.9 mm (3.4-11.2%), a significant increase in root length was not observed in this culture. On winter wheat, it is advisable to use the studied products separately. Sunflower is more responsive to the action of laboratory samples. They provided a shoot length of 13.6-14.6 mm (20.9-27.0%) and a root of 9.2-10.2 mm (4.5-15.9%). Organo-mineral fertilizers provided a shoot length of 14.2-14.5 mm (23.5-26.1%) and the root length of 10.8 mm (22.7%). Mixtures with WPU provided shoot length of 14.7-15.4 mm (27.8-33.9%), mixtures with WPB provided shoot length of 11.9-14.5 mm (3.5-26.1%), and no significant increase in root length was noted. The data obtained indicate the presence in the laboratory samples of fungicidal biological products growth-promoting properties, which vary depending on the culture and additionally used organic and mineral fertilizers. To confirm the results, additional experiments are necessary. Research in this direction will allow the rational use of the studied means of protection and increase plant yields in systems of organic, ecologized and integrated farming, and can help reduce the pesticidal pressure on agrocenoses.

Conflict of interest: the authors stated that there was no conflict of interest.

1. Kim K. H., Kabir E., Jahan S. A. Exposure to pesticides and the associated human health effects. Science of The Total Environment. 2017;575(1):525–535. DOI: https://doi.org/10.1016/j.scitotenv.2016.09.009

2. Perez-Montano F., Alias-Villegas C., Bellogin R., Cerro del P., Espuny M. R., Jimenez-Guerrero I., Lopez-Baena F., Ollero F., Cubo T. Plant growth promotion in cereal and leguminous agricultural important plants: From microorganism capacities to crop production. Microbiological Research. 2014;169(5-6):325-336. DOI: https://doi.org/10.1016/j.micres.2013.09.011

3. Pogacean M., Gavrilescu M. Plant protection products and their sustainable and environmentally friendly use. Environmental Engineering and Management Journal. 2009;8(3):607-627. DOI: https://doi.org/10.30638/eemj.2009.084

4. Azizbekyan R. R. Ispol'zovanie sporoobrazuyushchikh bakteriy v kachestve biologicheskikh sredstv zashchity rasteniy. [Application of sporiferous bacteria as agents for plant biological protection]. Biotekhnologiya = Biotechnology in Russia. 2013;29(1):69-77. (In Russ.). URL: https://elibrary.ru/item.asp?id=18819987

5. Maksimov I. V., Veselova S. V., Nuzhnaya T. V., Sarvarova E. R., Khay-rullin R. M. Stimuliruyushchie rost rasteniy bakterii v regulyatsii ustoychivosti rasteniy k stressovym faktoram. [Plant growth-promoting bacteria in regulation of plant resistance to stress factors]. Fiziologiya rasteniy = Russian Journal of Plant Physiology. 2015;62(6):763-775. (In Russ.). URL: https://elibrary.ru/item.asp?id=24149790

6. Kaushal M., Wani S. P. Rhizobacterial-plant interactions: Strategies ensuring plant growth promotion under drought and salinity stress. Agriculture, ecosystems and environment. 2016;231(1):68-78. DOI: https://doi.org/ 10.1016/j.agee.2016.06.031

7. Pravin V., Rosazlin A., Tumirah K., Salmah I., Boyce A. Role of plant growth promoting rhizobacteria in agricultural sustainability – a review. Molecules. 2016; 573 р. DOI: https://doi.org/10.3390/molecules21050573

8. Santoyo G., Moreno-Hagelsieb G., Orozco-Mosqueda M. del C., Glickc B. R. Plant growth-promoting bacterial endophytes. Microbiological Research. 2016;183:92-99. DOI: https://doi.org/10.1016/j.micres.2015.11.008

9. Freitas J. R., Germida J. J. Plant growth promoting rhizobacteria for winter wheat. Canadian journal of microbiology. 1990;36(4):265-272. DOI: https://doi.org/10.1139/m90-046

10. Siunova T. V., Anokhina T. O., Sizova O. I., Sokolov S. L., Sazonova O. I., Kochetkov V. V., Boronin A. M., Patil S. G., Chaudhari A. B. Shtammy PGPR Pseudomonas, perspektivnye dlya sozdaniya biopreparatov dlya zashchity i stimulyatsii rosta rasteniy. [PGPR pseudomonas strains promising for the development of bioformulations for plant protection and stimulation]. Biotekhnologiya = Biotechnology in Russia. 2017;33(2):56-67. (In Russ.). URL: https://elibrary.ru/item.asp?id=29143177

11. Ivanov A. I., Ivanova Zh. A., Freydkin I. A., Sokolov I. V. Vliyanie novogo organo-mineral'nogo udobreniya na izmenenie agrokhimicheskikh svoystv degradirovannoy dernovo-podzolistoy pochvy. [Influence of new organic-mineral fertilizer on the change of agrochemical properties of degraded sod-podzolic soil]. Agrokhimiya. 2019;3:30-36. (In Russ.). DOI: https://doi.org/10.1134/S0002188119030074

12. Ivanov A. I., Ivanova Zh. A., Sokolov I. V., Freydkin I. A. Novoe organo-mineral'noe udobrenie na posevakh zernovykh kul'tur. [The new organic-mineral fertilizer for grain crops]. Zernovoe khozyaystvo Rossii = Grain Economy of Russia. 2019; 3 (63): 64-68. (In Russ.). DOI: https://doi.org/10.31367/2079-8725-2019-63-3-64-68

13. Asaturova A. M., Tomashevich N. S., Zhevnova N. A., Krivoshlykov K. M., Khomyak A. I., Kozitsyn A. E., Dubyaga V. M., Sidorova T. M., Sidorov N. M., Tsygichko A. A., Bondarchuk E. Yu. Ekologizirovannaya sistema zashchity pshenitsy na osnove novykh original'nykh biofungitsidov. [Greenwheat protection system based onnew original biofungicides]. Tavricheskiy vestnik agrarnoy nauki = Taurida herald of the agrarian sciences. 2019;1(17):31-42. (In Russ.). DOI: https://doi.org/10.33952/2542-0720-2019-1-17-31-42

14. Kozitsyn A. E., Tomashevich N. S., Asaturova A. M., Sidorova T. M., Dubyaga V. M. Sovershenstvovanie elementov tekhnologii proizvodstva i primeneniya novykh effektivnykh biofungitsidov protiv parshi yabloni. [Improvement of technology elements of production and application of new efficient biofungicides against apple scab]. Plodovodstvo i yagodovodstvo Rossii = Pomiculture and small fruits culture in Russia. 2019;56:133-141. (In Russ.). DOI: https://doi.org/10.31676/2073-4948-2019-56-133-141

15. Maslienko L. V. Biologicheskiy metod zashchity podsolnechnika i drugikh sel'skokhozyaystvennykh kul'tur ot bolezney. [Biological method of protecting sunflower and other crops from diseases]. Agro XXI. 1999;8:9.

16. Parween T., Jan S., Mahmooduzzafar N., Fatma T., Siddiqui Z. Selective effect of pesticides on planta review. Critical reviews in food science and nutrition. 2016; 56: 160-179. DOI: http://dx.doi.org/10.1080/ 10408398.2013.787969