Molecular and biological characteristics of streptomyces diversity in the soils of the Saxaul forest in Mongolia
https://doi.org/10.30766/2072-9081.2021.22.1.85-92
Abstract
Saxaul (Haloxylon ammodendron) is a plant with a wide ecological range that forms forests in Mongolia often used as pastures for camels. Actinomycetes were isolated from the soils of the saxaul forest using selective isolation methods. According to the results of phylogenetic analysis based on the sequences of the 16S rRNA gene fragment, it has been established that the obtained isolates belong to the Streptomyces genus. According to the taxonomic position, strains (M41; M42; M 43; C 4-46; C 4-47; C 4-50; M44; C 5-54; C5-60 and C 5-63) demonstrate a high level of similarity (99.20- 100 %) of 16S rRNA gene sequences with type strains of the following species: S. fradiae, S. huasconensis, S. coeruleoprunus, S. tendae, S. rubrogriseus, S. malachitofuscus, S. flavoviridis S. pilosus, S. caelestis, S. azureus, S. fulvissimus, S. microflavus, S. griseussubsp. griseus, S. anulatus, S. cyaneofuscatus, S. luridiscabiei, S. halstedii, S. fulvorobeus, S. pratensis, S. setonii, S. anulatus, S. pratensis, S. caelestis and S. azureus.
Supporting agencies: The work was supported by the CAMS Innovation Fund for Medical Sciences (Grant No. CAMS 2017-12M-B & R-08) and by the Grant of the Mongolian Academy of Sciences 2018/10/. The authors thank Nikitina E. P., the junior researcher of The Baikal Institute of rational nature management SB RAS for her participation in the research. The authors thank reviewers for their contribution to the peer review of this work.
About the Authors
J. Norovsuren
The Institute of Biology, Mongolian Academy of Sciences
Mongolia
Mongolia
Jadambaa Norovsuren, DSc in Biological sciences
Peace Avenue-54B, Bayanzurkh District, Ulaanbaatar-13330
Liu Shao-Wei
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College
China
China
Liu Shao-Wei, Assistant Prof., Institute of Medicinal Biotechnology
Beijing, 100050
Sun Cheng-Hang
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College
China
China
Sun Cheng-Hang, Professor and Dean of Department of Microbial Chemistry, Professor
Beijing, 100050
B. Altansukh
The Botanic Garden and Research Institute, Mongolian Academy of Sciences
Mongolia
Mongolia
Batbaatar Altansukh, master, researcher
Peace Avenue-54А, Bayanzurkh District, Ulaanbaatar-13330
Ch. Dorjsuren
The Botanic Garden and Research Institute, Mongolian Academy of Sciences
Mongolia
Mongolia
Chimidnyam Dorjsuren, Professor, Academician
Peace Avenue-54А, Bayanzurkh District, Ulaanbaatar-13330
References
1. Dorzhsuren Ch. Antropogennye suktsessii v listvennichnykh lesakh Mongolii: monografiya. [Anthropogenic successions in leafy forests of Mongolia: monograph]. Moscow: Izd-vo Instituta problem ekologii i evolyutsii im. A. N. Severtsova, 2009. p. 209.
2. Gal Zh. Ob izuchenii saksaulovykh pastbishch i ikh botanicheskie kharakteristiki. [On the study of saxaul pastures and their botanical characteristics]. Trudy instututa biologii AN MNR. 1969;(3):177-191. (In Mongolia).
3. Tsatsenkin I. A., Yunatov A. A. Estestvennye kormovye resursy Mongol'skoy narodnoy respubliki. Vostochnaya chast' Gobi. [Natural food resources of the Mongolian People's Republic. The eastern part of the Gobi]. Trudy mongol'skoy komissii. [Proceedings of the Mongolian Commission]. Moscow: izd-vo ANSSR,1951. Iss. 40. 350 p.
4. Yunatov A. A. Zametki o saksaule v Mongolii. [Notes on saksaul in Mongolia]. Uchenye zapiski Mongol'skogo GU. Ulan – Bator, 1946. Iss.1. pp. 93-97. (In Mongolia).
5. Kalakutskiy L. V., Agre N. S. Razvitie aktinomitsetov. [The development of actinomycetes]. Moscow: Nauka, 1977. 287 p.
6. Zvyagintsev D. G., Zenova G. M. Ekologiya aktinomitsetov. [Ecology of actinomycetes]. Moscow: GEOS, 2001. 257 p.
7. Hayakawa M., Nonomura H. Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. Journal of Fermentation Technology.1987;65(5):501–509.
8. Zenova G. M. Pochvennye aktinomitsety redkikh rodov. [Soil actinomycetes of rare genera]. Moscow, 2000. 81 p.
9. Gauze G. F., Preobrazhenskaya T. P., Sveshnikova M. A., Terekhova L. P., Maksimova T. S. Opredelitel' aktinomitsetov. [The determinant of actinomycetes]. Moscow, 1983. 245 p.
10. Shirling E. B.,Gottlieb D. Methods for characterization of Streptomyces species. International Journal of Systematic Bacteriology. 1966;16(3):313-340. DOI: https://doi.org/10.1099/00207713-16-3-313
11. Opredelitel' bakteriy Berdzhi: V 2 t. Pod red. Dzh. Khoula i dr. [Bergie's Bacterial Determinant: In 2 vols. Edited by J. Howl et al.]. Moscow, 1997. 800 p.
12. Hasegawa Т., Takizawa M., Tanida S. A rapid analysis for chemical grouping of aerobic actinomycetes. J. Gen. Appl. Microbiol. 1983;29(4):319-322. DOI: https://doi.org/10.2323/jgam.29.319
13. Li W. J., Xu P., Schumann P., Zhang Y. Q., Pukall R., Xu L. H., Stackebrandt E., Jiang Ch. L. Georgenia-ruaniisp.nov., anovelactinobacterium isolated from forest soil in Yunnan (China) and emended description of the genus Georgenia. Int. J. Syst. Evol. Microbiol. 2007;57(7):1424–1428. DOI: https://doi.org/10.1099/ijs.0.64749-0
14. Liu Q. Q., Wang Y., Li J., Du Z. J., Chen G. J. Saccharicriniscarchari sp. nov., isolated from a shark, and emended descriptions of the genus Saccharicrinis and Saccharicrinisfermentans. Int. J. Syst. Evol. Microbiol. 2014;64(7):2204-2209. DOI: https://doi.org/10.1099/ijs.0.061986-0
15. Yoon S. H., Ha S. M., Kwon S., Lim J., Kim Y., Seo H., Chun J. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int. J. Syst. Evol. Microbiol. 2017;67(5):1613‒1617. DOI: https://doi.org/10.1099/ijsem.0.001755
16. Kumar S., Stecher G., Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution. 2016;33:1870-1874. DOI: https://doi.org/10.1093/molbev/msw054
17. Saitou N., Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. MolBiolEvol. 1987;4(4):406–425. DOI: https://doi.org/10.1093/oxfordjournals.molbev.a040454
18. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution. 1980;16:111-120. DOI: https://doi.org/10.1007/BF01731581
19. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution. 1985;39:783-791.
20. Norovsuren J., Kostina N. V. Redkiy rod aktinomitsetov Actinomadurasp. v pochvakh saksaul'nogo lesa (Haloxylon ammodendron) Mongolii.Doklady TSKhA: sb. statey. [A rare genus of actinomycetes, Actinomadurasp. in the soils of the saxaul forest (Haloxylon ammodendron) Mongolia.Reports of the TSHA: collection of articles. Moscow: Publishing House of the Russian State Agrarian University-MSHA named after K. A. Timiryazev]. Moscow: Izd-vo RGAU – MSKhA imeni K. A. Timiryazeva, 2020. Iss. 292. Part. IV. pp. 78-80. URL: https://www.timacad.ru/uploads/files/20200706/1594041128_vipusk_292-IV.pdf
21. Norovsuren J., Shul'ga-Mikhaylova N. V., Zenova G. M., Zvyagintsev D. G. Aktinomitsety v pochvakh Mongolii. [Actinomycetes in the soils of Mongolia]. Pochvovedenie = Eurasian Soil Science. 2002;(2):198-204. (In Russ.).
22. Aidan C. Parte. LPSN−list of prokaryotic names with standing in nomenclature. Nucleic Acids Research. 2014;42(D1): D613-D616. DOI: https://doi.org/10.1093/nar/gkt1111
23. Doroshenko E. A., Zenova G. M., Zvyagintsev D. G., Sudnitsyn I. I. Prorastanie spor i rost mitseliya streptomitsetov pri raznykh urovnyakh vlazhnosti. [The germination of spores and growth of mycelium of Streptomyces under different humidity levels]. Mikrobiologiya = Microbiology. 2005;74(6):795-799. (In Russ.).
24. Doroshenko E. A., Norovsuren J., Zenova G. M. Prorastanie spor i rost mitseliya aktinomitsetov, vydelennykh iz pochv Mongolii, pri raznykh urovnyakh vlazhnosti. [Spore germination and mycelium growth of actinomycetes isolated from Mongolian soils at different humidity levels]. Chetvert' veka na strazhe plodorodiya: mat-ly Mezhdunarodn. nauchn.-prakt. konf. [A quarter of a century on the guard of fertility: Proceedings of International scientific and practical Conf.]. Belgorod, 2006. pp. 82-84.
25. Eftekharivash L., Hamedi J., Zarrini Gh., Bakhtiari R. Introducing acidophilic and acid tolerant actinobacteria as new sources of antimicrobial agents against Helicobacter pylori. Archives of Razi Institute. 2020;76(2). DOI: https://doi.org/10.22092/ari.2019.128039.1401
26. Arai Y., Iinuma H., Ikeda Y., Igarashi M., Hatano M., Kinoshita N., Ukaji T., Simizu S., Umezawa K. Migracins A and B, new inhibitors of cancer cell migration, produced by Streptomyces sp. J. Antibiotics (Tokyo) 2013;66(4): 225−230. DOI: http://dx.doi.org/10.1038/ja.2012.112
Review
For citations:
Norovsuren J., Shao-Wei L., Cheng-Hang S., Altansukh B., Dorjsuren Ch. Molecular and biological characteristics of streptomyces diversity in the soils of the Saxaul forest in Mongolia. Agricultural Science Euro-North-East. 2021;22(1):85-92. https://doi.org/10.30766/2072-9081.2021.22.1.85-92
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