The review informs on the results of the research of the application of inulin as a bioactive prebiotic in functional nutritional products, in cosmetic formulations and pharmaceuticals as the excipient of anticancer agents on the basis of the system analysis. The steady trend of the world growth of inulin production of 8-10 % a year has been noted. The aim of the research is to reveal and to analyze the effect of the system complex of inulin production in sequential chain from the inulin synthesis, its accumulation in plants and extraction. The Jerusalem artichoke is the most advanced kind of raw material which has the high crop yield of tubers up to 40 t/ha and inulin content up to 20 %. The sucrose and the synthesizing enzymes 1-SST and 1-FFT take an active part in the inulin biosynthesis as their genes can be edited with the purpose of changing the inulin content. At the Jerusalem artichoke breeding the most prospective trend is the cross-species hybridization drawing on the example of a topinsunflower with the crop yield of tubers of 40 t/ha. In the Jerusalem artichoke seed breeding the new methods of clonal micropropagation of tubers and its cultivation in the airhydroponic environment with the achievement of the inulin content no less than 20 % have been studied. At Jerusalem artichoke cultivation the breed is significative for the maximum crop yield of tubers with the row width of 90 cm and with the interval between tubers in a row – 30 cm. The least loss of the tubers weight and the inulin content in them under the long-term storage is achieved at the temperatures from -5 to 0 °C. The technology of the Jerusalem artichoke tubers processing into inulin is suggested as the system of partly optimized technological operations from peeling and washing the tubers to receiving the powdered inulin and the oligofructose syrup. The system complex is suggested in the form of the structural topological model combining 4 systems: «Selection and seed breeding», «Agritechnologies», «Harvesting and storage of tubers», «The tubers processing technologies» with the interrelations in the form of the technological requirements and the output data of every system and of the complex as a whole, which is the basis for the development of the agri-food technology of inulin from Jerusalem artichoke and other kinds of raw materials.

Potato virus Y (PVY) is an economically important pathogen of potato as a vegetatively propagated crop. High resistance to all strains of PVY is determined by Ry-genes, which are introgressed into modern potato varieties from a limited number of sources of resistance ‒ Solanum stoloniferum Schlechtd. et Bche., Solanum andigenum Juz. et Buk., Solanum chacoense Bitt. Use of new species Solanum and interspecific hybrids based on them provides for the expansion of existing potato gene pool in breeding for resistance to PVY. Traditional breeding for resistance to viruses has still a great potential, though, it is a long and laborious process. Molecular markers linked to Ry-genes are widely integrated in order to increase the effectiveness of practical breeding. Previous studies have revealed a number of shortcomings in using the molecular markers of Ry-genes. To assess the predictive abilities of molecular markers RYSC3, M45, M6 of the Ryadg gene and YES3-3A of the Rysto gene for resistance to PVY, the F1 generation of two potato populations was studied, in the creation of which inter-specific hybrids were used. The nature of segregation 5:3 obtained by phenotype showed that the original parental forms can be the sources of not only previously identified, but also unidentified Ry-genes and Ny-genes of hypersensitivity. Correlation coefficient between the presence of markers and resistance to PVY was 0.64 for the YES3-3 marker (79 % matching) and 0.54 for RYSC3, М45, М6 markers (76 % matching). There have been revealed the cases of “false positive” results of the study (the presence of a marker in susceptible genotypes), which indicate to the insufficient effectiveness of the markers used. The marker segregation observed in the populations was consistent with chromatid segregation, confirming the simplex nature of Ry-genes inheritance from resistant parents. The ratio of genotypes with the presence/absence of markers was 0.86:1.

Prolonged inundation of soybean plants leads to their death because of root hypoxia. Therefore, the search for markers of resistance to this factor will provide the opportunity to develop the varieties able to resist this stress. The research was aimed at the study of response of 3 varieties bred by Federal Research Center All-Russian Scientific Research Institute of Soybean to prolonged flooding according to the indicator of chlorophyll a and b content in soybean leaves. The study was carried out in 2019-2021 in laboratory experiments when growing plants on hydroponic installations with 24-day soil flooding (control - soil with optimal moisture content). In soybean varieties Evgeniya and Kukhanna, registered as resistant to overwatering soil, the response by content of chlorophyll forms in leaves under 24-day flooding of the soil surface with 1-2 cm layer of water differed. It was found that increase of chlorophyll a in leaves of Evgeniya variety under flooding was connected with providing the ability of this variety plants to resist the stressful flooding factor. The presence of chlorophyll b in the leaves of this variety before the ripeness phase indicates that it can serve as an indicator of plant resistance to root hypoxia to a greater extent, since the plants continued to grow and develop. In the Kukhanna variety, the content of both forms of chlorophyll at optimum soil moisture of the control variety was higher or at the same level as in the variant with flooding, that cannot serve as the criterion of resistance of this variety to root hypoxia. In the Kitrossa variety, the resistance of which to overwatering has not been noted by the breeders yet there were revealed wavy fluctuations in chlorophyll a/b ratio during the first 10 days of flooding. The fluctuations were caused by changes in the content of chlorophyll b, the concentration of which increased first and then decreased. This fluctuation is most clearly seen in the graph of the change in the ratio between chlorophyll a and b, that may serve as a marker for determination of resistance of soybean varieties to prolonged soil flooding.

The article provides the data of the field experiment (2016-2019) on studying soybean varieties in order to determine their suitability for cultivation in Kaluga region (the central part of the Non-Chernozem region of the Russian Federation) and their use as a source of agronomic traits for further breeding work. The objects for the research were the soybean varieties of Russian (Mageva, Svetlaya, Kasatka, Maleta, Okskaya, Georgia) and Belarusian (Pripyat and Volma) breeding. Cultivation of soybean varieties in the northern regions of the country is limited due to the short growing season. During the research, the source of early ripening trait has been identified – Kasatka variety had the shortest vegetation period (109 days, 10 days less than Mageva standard variety). According to the plant height Pripyat (93.5 cm) and Okskaya (92.7 cm) varieties have been noted. In comparison to the control, Maleta, Pripyat and Volma varieties showed a steady dynamics towards dry matter accumulation increase. Plant yield index is the most important indicator in assessing soybean varieties genotype suitability for the certain cultivation area. A close correlation has been established between Svetlaya and Okskaya varieties yield indices and moisture availability indicators during the growing season (the amount of precipitation and the hydrothermal coefficient) (r = 0.9). The yield of the Belarusian Volma and Pripyat varieties showed a strong correlation between the average density and precipitation (r = 0.5-0.7) and HTC (r = 0.4-0.6), which proved the possibility of their cultivation in the Non-Chernozem region. On average over the research Pripyat variety has been the most productive one (2.0 t/ha, 21 % higher than the standard), which makes it useful as a source for increased productivity trait in soybean breeding.

The purpose of the study was to create of an early ripening variety of narrow-leaved lupine with a seed and green mass yield above the standard, resistant to unfavorable biotic and abiotic environmental factors in the North-West region of the Russian Federation. As the result of many years of breeding, an early ripening variety of narrow-leaved lupine Metsenat has been developed. In a competitive varietal trial conducted in the conditions of the Leningrad Region, the seed yield of the variety Metsenat averaged 4.3 t/ha in 2015-2017 that was 1.1 t/ha higher than the standard (Kristall), the yield of green hay was 48.4 t/ha (13.9 t/ha higher than the standard). According to the results of the structural analysis of plants, the variety Metsenat was characterized by an increased number of seeds per plant (90.4 pcs.) and a good seeding of the beans (4.4 pcs.). It is a variety of universal use, with a low content of alkaloids (0.0072 %), it is technological, resistant to lodging (7 points), to cracking of beans and shedding of seeds on the vine, resistant to anthracnose (9 points). It is characterized by a rapid growth rate of plants in the initial period of vegetation. In 2018 the Metsenat variety was included into the State Register of Breeding Achievements of the Russian Federation approved for use in agricultural production in 12 regions (Patent No. 9559).

The research is devoted to the optimization of the method of adaptation of Reine Sammut microplants of roses using silicon oxide (SiO2) by spraying and watering. For the experiment, there have been used standard microplants obtained by the method of clonal micropropagation, corresponding to GOST 29105.1-91-29105.3-91. Before planting for adaptation, the microplants were cleared from the lower leaves, the roots were washed from the agar nutrient medium in a decimolar solution of potassium permanganate and trimmed to 15-20 mm. Adaptation was carried out in micro-greenhouses on a peat nutrient substrate, made in accordance with TU 20.12.80 001 41790563 2020, in a light room (humidity 36 %, temperature 23...25 ° C, light period duration 16 hours, illumination 8000 Lx). In order to disinfect the substrate, the biofungicide "Trichoderma veride" (1.5 ml/l) was used. Microplants after planting for adaptation were shed and sprinkled in accordance with the experiment options: 1) distilled water (control); 2) 0.01% SiO2 solution; 3) 0.005% SiO2 solution; 4) 0.0025% SiO2 solution. With prolonged ventilation of micro-greenhouses, 28 % of plants in the control variant showed drying of the leaves, which did not occur when using silicon oxide solutions. According to visual diagnostics, the most attractive plant species was when watered and sprayed with 0.01% silica. This concentration of silicon oxide during plant adaptation compared to the control contributed to a significant increase in plant height and shoot length by 50.0 mm and 42.6 mm, respectively (LSD05 = 36.1 and 28.3), and a tendency to increase the number of leaves by 1. 6 pcs., shoot weight 2.0 and roots 1.8 times.

Currently, the main methods of virus elimination of fruit crops are dry-air thermotherapy, meristem culture and chemotherapy. The use of a complex of physical and chemical methods makes it possible to increase the efficiency of virus elimination. Improving the methods of recovery must be carried out in relation to certain types of viruses, the biological characteristics of the crop and variety. The purpose of the research is to study the effect of the Pharmaiod preparation on biometric, physiological parameters and the release of harmful viruses-free pear plants in the process of dry-air thermotherapy. Improvement of pear plants of varieties Letnyaya Zabava, Zolotoy Vityaz, hybrids R-11-9, R-10-3 and R-2-4 from harmful latent viruses of Apple stem grooving virus (ASGV), Apple stem pitting virus (ASPV), Apple chlorotic leaf spot virus (ACLSV), Apple mosaic virus (ApMV) were carried out in 2020-2021 at the temperature of 38 °C for 3 months using a thermal chamber designed by the Federal Horticultural Research Centеr for Breeding, Agrotechnology and Nursery. Biometric parameters in pear plants depended on varietal characteristics, the duration of therapy and the concentration of the Pharmaiod preparation. In most of the varieties and forms studied, after 3 months of thermotherapy, the Pharmaiod preparation at a concentration of 1 ml/l contributed to an increase in the length of 1 shoot of pear plants by 1.4-3.1 times compared with the control. Under the conditions of a thermal chamber, according to a set of indicators, pear hybrids R-11-9 and R-10-3 were characterized by the highest heat resistance; medium heat resistance was shown by Letnyaya Zabava and Zolotoy Vityaz varieties; low – by R-2-4 hybrid. Higher heat-resistant varieties and forms were characterized by a more restrained growth of shoots in length compared to lower heat-resistant ones. An average negative significant correlation at the 5% significance level was established between the water content in the leaves and the length of 1 shoot (r = -0.52). The yield of virus-free pear plants depended on the type of virus. The yield of pear plants free from 4 major harmful viruses as a result of dry-air thermotherapy without the use of the Pharmaiod preparation was 50 %, with the use of the drug it was 60 %.

The aim of the study was to analyze the resistance of 21 Microdochium nivale strains (fungi that cause pink snow mold in winter crops), living within a single area and a single crop (winter rye), to fungicides that differ in chemical nature and mechanisms of action as well as to test the interconnection between levels of virulence and fungicide resistance of strains. The virulence of M. nivale strains was determined on detached leaves of rye (Ogonek variety) plants as well as on whole plants grown under sterile conditions in vitro. The resistance of strains to fungicides (Provisor (a.s. azoxystrobin) and Ferazim (a.s. carbendazim)) was determined by inhibition of mycelium growth. As a result of the experiments, more strains (13) capable of growing in the presence of Provisor were revealed than Ferazim-resistant strains (2); however, Ferazim-resistant strains had a greater level of resistance (5-16% of growth inhibition) than Provisor-resistant strains (63-94 % of growth inhibition). A negative correlation (Spearman's correlation coefficient -0.604 and -0.532) between the level of virulence of M. nivale strains and the level of their susceptibility to Provisor was found. This evidently means that the acquisition of resistance to Provisor by M. nivale strains is accompanied by an increase in their virulence. No correlations were found between the fungicide resistance of M. nivale strains and their attribution to one or another phylogenetic group, to which the strains were assigned based on the nucleotide sequence of the ITS2 (internal transcribed spacer 2) region. The study shows that for choosing the fungicide application strategy, it is necessary to analyze phytopathogen populations for the presence of strains that are simultaneously characterized by high virulence and resistance to various fungicides and also take into account that the adaptation of fungi to certain fungicides can be accompanied by an increase in their virulence, which will negatively affect the phytopathological state of agrocenosis.

The study of biochemical quality indicators of 26 new potato hybrids was carried out in order to determine the ones mostly suitable for processing into starch and potato products and for use as table variety. An express method was used to determine dry matter (DM), Evers method was used to determine the total starch content of tubers, the polarimetric method and a glucometer test were used to determine reducing sugars, glycoalkaloids and inorganic phosphorus were determined by spectrophotometric method. There have been selected 5 hybrids suitable for industrial processing and as a source material for breeding. One potato hybrid with 25,06 % content of DM, more than 18,22 % of starch met the requirements for potatoes used for processing into starch and starch products; another potato hybrid can be recommended for the production of fried potato products according to the parameters: DM – 22.40 % starch – 16.18 %, reducing sugars – 0.23 %, glycoalkaloids – 62,0 mg/kg. Three of the studied hybrids with the content of DM of more than 22 %, starch not less than 16%, reducing sugars 0.2-0.4 % and glycoalkaloids 60-126 mg / kg may be used for the production of other types of potato products. The rest of the hybrids can only be recommended for use as table potatoes. The relationship between indicators affecting the quality of potato products has been revealed. Correlations were established between the mass fractions of: dry matter and starch in the tuber (r = 0.98) – high correlation; reducing sugars and glycoalkaloids (r = 0.68); tuber dry matter and glycoalkaloids (r = 0.59); dry matter and glucose (r = -0.61); starch and glucose (r = -0.58) – average correlations. It has been established that the mass fraction of reducing sugars and the direction of rotation of the plane of polarization of light by sugars change non-linearly under different temperature conditions of sample storage.

The purpose of the research is to study the influence of hydrothermal conditions of vegetation of 2011-2022 on the yield of spring barley of the Rodnik Prikamya variety in the conditions of the Middle Trans-Urals. Barley was cultivated in a traditional field grain-grassfallow seven-field crop rotation against two backgrounds of mineral nutrition (without fertilizers, N60P30K60). In the conditions of a long stationary experiment, the influence of indicators of moisture and heat supply of vegetation periods (air temperature, precipitation amount, G. T. Selyaninov hydrothermal coefficient) and mineral fertilizers on barley yield was analyzed. The analysis proves the reliability of the relationship, and the regression equations for the curvilinear dependence make it possible to predict the yield of spring barley for both backgrounds of mineral nutrition in typical soil and climatic conditions for the Middle Trans-Urals. For the variant with the introduction of mineral nutrition, with a prediction accuracy of 84.56 %: Y = -1.22163X2 + 0.69X + 4.01388626, where Y is the predicted yield, t/ha, X is the hydrothermal coefficient of May. For the variant without mineral fertilizers: Y = -0.080X2 + 0.5844X + 2.5506, where Y is the predicted yield, t/ha, X is the hydrothermal coefficient of June. The accuracy of forecasting the yield of spring barley by the hydrothermal coefficient of June is 77.89 %.

The article provides the research of 2011-2019 on studying the long-term influence of the aftereffect of 36-year application of mineral fertilizers on the productivity and nutritional value of long-term meadow phytocenoses. In a stationary experiment with a radical improvement of meadows, the following variants for applying mineral fertilizers were studied from 1974 to 2010: the minimum level was N62P60K11 (control), the average level was N202P175K43, and the maximum level was N318P362K189. In the conditions of the Privilyui agrolandscape of the middle taiga subzone of Yakutia, a long-term aftereffect of fertilizers (from 2011 to 2019) contributed to the preservation of the main dominant in the grass stand - couch grass (Elymus repens (L.) Nevski) up to 27.4-42.1 % and codominant – short-awned barley (Hordeum brevisubulatum (Trin) Link.) up to 14.7-24.3 %. During the aftereffect of fertilizers, the yield of cereal-forb phytocenosis averaged 1.71-2.03 t/ha of hay, which is 2.1-2.5 times higher than the yield of natural meadows. At the same time, hay forage contained up to 8.9-9.1 MJ of exchange energy, up to 0.62-0.65 feed units, and up to 80-90 g of digestible protein, which corresponds to the zootechnical norm.

The article presents the results of studies on the effect of feeding doses of vitamin-herbal flour made of the green mass of R. carthamoides on the growth dynamics and biochemical composition of the blood of young cattle. The studies were carried out in 2022 at the dairy complex of Sokolovo LLC (Perm Krai). The objects of the study were replacement heifers of the Holsteinized Black-and-White breed at the age of 12-15 months. For 92 days, heifers of I and II experimental groups were additionally fed vitamin-herbal flour made of R. carthamoides in the dose of 150 and 300 g per head per day as part of the main diet. The use of nitrogen from the incoming and from the digested one was more in heifers of the I and II experimental groups by 0.74-1.09 %abs. (p < 0.05) and 2.17-3.64% abs. (p < 0.01) compared with the control group. At the age of 15 months, heifers of I and II experimental groups in terms of live weight exceeded the animals of the control group by 2.80 and 18.20 kg (p < 0,001) by asbsolute gain by 4.86 and 11.61% respectively. At the end of the experimental period, in the blood serum of heifers of the II experimental group, where the maximum dose of vitamin-herbal flour was used, the content of total protein increased by 11.43 %, albumin ‒ by 14.01 %, carotene - by 28.57 %, the level of reserve alkalinity ‒ by 18.01 %. In all cases, the difference with the control was confirmed statistically (p < 0.05-0.001). Thus, the studies carried out testify to some bioavailability of vitamin-herbal flour and its positive effect on the physiological state of experimental animals, which consists of the normalization of metabolism and improvement of biochemical parameters of blood, which makes it possible to recommend introducing vitamin-herbal flour made of R. carthamoides into the diets of young cattle at the rate of 300 g per head per day.

The aim of the study is to identify the frequency of occurrence of various allelic variants and genotypes of beta-casein in cows of the Kholmogory breed and their relationship with dairy productivity. The tasks of the research are genotyping of cattle of the Kholmogory breed by the beta-casein locus and establishing its connection with qualitative and quantitative indicators of dairy productivity. As the objects for the research there were taken 150 cows of the 1st, 2nd and 3rd lactation. An allele-specific variant of the PCR method (AS-PCR) was used to identify A1 and A2 beta-casein. As the result it had been established that in the studied part of the herd, 23 % of animals had the A2A2 genotype, 43 % of animals had the A1A1 genotype and 34 % of animals had the A1A2 genotype. For 100 days of the first lactation, animals with A1A2 genotype showed the highest value in milk yield. Animals with A2A2 genotype for 305 days of lactation had the highest milk yield and the amount of milk protein, however, the difference was not statistically significant compared to the animals with A1A2 genotype. Genotype A1A1 has lower indicators by all the parameters studied, with a significant difference relative to genotypes A1A2 and A2A2. Thus, the study of CSN2 is a promising area of scientific research, and the results of the study of beta-casein genotypes can be used as a marker selection in improving the herds of the Kholmogory breed.

Conducting genetic studies of domestic local breeds of cattle is relevant due to the fact that they are carriers of valuable economic traits and have a high adaptive ability to local conditions. The aim of the research is to study the association of polymorphic variants of the leptin gene with the reproductive ability of cattle of the Kostroma, Black-and-White and Yaroslavl breeds bred in the Kostroma region. Genotyping was performed by real-time polymerase chain reaction (RT-PCR) using HRM analysis. It was found that in the Kostroma and Yaroslavl breeds, the AV genotype (0.546 and 0.452, respectively) had the highest frequency for the LEP-A80V locus, and the AA genotype (0.550) in the Black-and-White breed. In terms of polymorphism Y7F of the leptin gene, the YY genotype prevailed among animals of all the studied breeds of cows. For the LEP-R25C locus, the highest frequency of occurrence was recorded in individuals with the RC genotype (0.486), while in cattle of the Yaroslavl and Black-and-White breeds, the RR genotype (0.690 and 0.483, respectively). However, no statistically significant differences were found in terms of reproductive ability between cows of different genotypes for the leptin gene. There is reason to believe that in cattle of the Kostroma breed the desired genotype is AV, in Yaroslavl cattle it is AA (LEP-A80V), and in Black-and-White cattle it is RR (LEP-R25C). The observed tendencies towards the presence of higher reproductive qualities in the carriers of the LEP-A80VA and LEP-R25CR alleles in the studied cattle breeds are confirmed by the studies of other authors. Therefore, the study of the effect of leptin gene polymorphism on the reproductive abilities of cows of domestic dairy breeds must be continued with the involvement of a significantly larger number of animals.

The purpose of the research was to study the main indicators of the reproductive ability of Black-and-White cows, depending on the proportion of Holstein genes. As the material for the research there were taken the data of breeding and zootechnical records of cows of Black-and-White breed of CJSC Semenovsky Breeding Plant of the Mari El Republic. The results of the research showed that with an increase in the proportion of thorough-bredness on the Holstein breed the reproductive ability of cows improved. Thus, the first-calf heifers with a low proportion of Holstein genes (up to 12.5 %) had the latest age of the first fruitful insemination and the first calving (21.9 months and 32.3 months). With an increase in thorough-bredness up to 87.6 % and more, the age for the first insemination decreased by 7.7 months (31.8 %), the age of the first calving - by 7.4 month (21.8 %), fertility index increased by 5.8 (14.6 %). At the same time, the duration of the service period in high-blooded animals increased by 18.8-26.4 days (15.8-23.8 %). The duration of the calving interval in all genetic groups exceeded the optimal time and changed in accordance with the change in the service period. The longest calving interval was in cows with the thorough-bredness degree of 87.6 % (13.8 months), which was 0.1-0.7 months longer than in animals of other genetic groups. There was a low coefficient of reproductive ability of the studied animals of all genetic groups ‒ 0.91-0.93. The significant influence of Holstein genes on the age for the first fruitful insemination (ɳ² = 9.57 %), the first calving (ɳ² = 8.80 %) and the fertility of cows (ɳ² = 1.98 %) (P≤0.05) was established. The effect of the genotype on the duration of the service period and calving interval was weak (ɳ² = 0.09 % and ɳ² = 0.26 %) and unreliable.

The article presents the data on the effect of the Lamarin Saldonum additive on reproductive function and milk productivity of cows when used in different periods: 30 days and 60 days before calving (groups S1 and S2), beginning from the day of calving (group S3) compared with the control (group C4). Highly productive black and white Holstein breed cows were used in the experiment. The additive was fed at a dose of 0.4 g per 1 kg of live weight during 60 days. It was found that S2 group had significantly shorter duration of pregnancy than S1 and C4 groups by 6.2 and 8.2 days, respectively, remaining within the limits of the physiological norm. The shortest indifference-period compared to C4 was observed in groups S1 and S3, the significant difference being 15.25 % and 18.39 %, respectively. The least number of days from calving to fruitful insemination was recorded in group S1, compared to control the statistically significant differences were 37.16 %, compared to groups S2 and S3 – 28.82 % and 27.67 %, which reliably reduced the period of infertility by 57.33 % relative to the values of group C4, as well by 47.90 % and 46.49 % relative to the indicators of groups S2 and S3. The highest average daily milk yields were recorded in group S1: in the first month of lactation, the difference with groups S2, S3 and C4 was 5.74 %, 14.01 % and 17.06 % (psecond (at p0.05), 14.53 %, 16.52 %, 15.19 %, in the third 9.57 % (p0.05), 13.06 %, 10.94 % (p0.05), in the first 100 days of lactation 11.90 %, 16.86 % (p0.05) and 13.28 % (p0.01), respectively. Thus, the most optimal scheme of application of Lamarin Saldonum for stimulation of reproductive function and milk productivity of cows is 30 days before the expected calving.

The purpose of the study was to evaluate the effect of the technological operation “pre-slaughter fasting period” on the qualitative characteristics of the meat of young bulls. The studies were carried out in two groups of Ayrshire bulls of 18 months of age, with a live weight of 435 ± 3.25 kg: I (n = 10) – slaughter without fasting period at the slaughter station, II (n = 10) – slaughter with 24-hour fasting period at the slaughter station. The total distance from the farm to the animal weighing and slaughter station was 97 km, and the travel time was 1 hour 43 minutes. The ambient temperature at the time of transportation was minus 13-15 °C, air humidity was 79 %, wind speed was 6 m/s. It has been established that the technological operation in the form of fasting period during 24 hours after transportation affected the qualitative indicators of the longissimus dorsi muscle, namely, there increased: the dry matter content by 2.45 %, protein – by 2.64 %, glycogen concentration – by 38.9 %, moisture capacity – by 11.01 %, oleic fatty acid – by 2.65 % with a decrease in moisture content – by 2.48 %, pH – by 3.5 %, myristic fatty acid by 0.95 %, palmitoleic acid – by 0.42 %, stearic acid – by 1.35 % compared to the group of bulls without fasting period. Thus, pre-slaughter 24-hour fasting period of young bulls improves the qualitative characteristics of meat compared to animals slaughtered without it.